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Selection of mandelamidase constitutive mutants in continuous culture ofPseudomonas putida

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Summary

A method is described for the isolation of mandelamidase constitutive mutants ofPseudomonas putida in continuous culture. Growth was nitrogen-limited and the nitrogen was provided either as inducing substrate (mandelamide) or non-inducing substrate (isobutyramide) alternately.

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Authors and Affiliations

  1. Department of Biochemistry, University College London, WC1E 6BT, London

    Peter D. Laverack & Patricia H. Clarke

Authors
  1. Peter D. Laverack
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  2. Patricia H. Clarke
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Laverack, P.D., Clarke, P.H. Selection of mandelamidase constitutive mutants in continuous culture ofPseudomonas putida . Biotechnol Lett 1, 353–358 (1979). https://doi.org/10.1007/BF01386699

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  • DOI: https://doi.org/10.1007/BF01386699

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