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In Vitro Conservation of Date Palm Germplasm

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Date Palm Biotechnology

Abstract

Date palm (Phoenix dactylifera L.) germplasm is difficult to conserve and store in the form of offshoots or in field collections. Tissue culture technologies have had a major impact on the ex situ conservation of plant genetic resources. In vitro culture techniques supplement date palm conservation efforts and have been applied to germplasm collection, preservation and rapid clonal multiplication. In vitro storage methods have been developed for preservation of date palm germplasm and can be used efficiently for international exchange of germplasm because of their obvious advantages over in vivo material. Preservation of plant cells, meristems and somatic embryos has become an important tool for long-term storage of germplasm utilizing minimum space and low maintenance. Short- and mid-term storage is achieved by controlling environmental growth conditions and nutrient media composition. Long-term storage has been reported for in vitro cryopreservation of date palm cultures. Encapsulation of plant material in alginate beads has been suggested recently as a possible means of date palm germplasm exchange. Knowledge about germplasm diversity and genetic relationships are highly valuable tools in plant conservation strategies. In this regard, a number of molecular biology methods are currently available for analysis of genetic diversity in date palm genotypes. This chapter discusses the general issues and different aspects of plant biotechnology used for management and conservation of date palm cultivars.

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  1. Plant Biotechnology Department, National Research Center, El-Tahrir Str., Dokki, 12622, Cairo, Egypt

    S. A. Bekheet

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  1. , Department of Applied Biology, University of Helsinki, Latokartanonkaari 5, Helsinki, 00014, Finland

    Shri Mohan Jain

  2. , Department of Agricultural Biotechnology, King Faisal University, Al-Hassa, 31982, Saudi Arabia

    Jameel M. Al-Khayri

  3. Middlebrook Ave 3726, Cincinnati, 45208, Ohio, USA

    Dennis V. Johnson

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Bekheet, S.A. (2011). In Vitro Conservation of Date Palm Germplasm. In: Jain, S., Al-Khayri, J., Johnson, D. (eds) Date Palm Biotechnology. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-1318-5_17

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