Abstract
Genetic modification of dedicated bioenergy crops is in its infancy; however, there are numerous advantages to the use of these tools to improve crops used for biofuels. Potential improved traits through genetic engineering (GE) include herbicide resistance, pest-, drought-, cold- and salt-tolerance, lower inputs, compositional alterations, addition of cellulases and other biofuels-specific traits such as increased biomass yields and increased photosynthetic efficiencies. To achieve these goals on an agricultural scale, these improvements must meet regulatory standards for release into the environment. In most cases, these criteria will probably require gene confinement strategies to prevent gene flow into wild and non-transgenic populations. Here, we consider the options for prevention or mitigation of gene flow in genetically modified (GM) biofuels crops.
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References
Albert H, Dale EC, Lee E, Ow D (1995) Site-specific integration of DNA into wild-type and mutant lox sites placed in the plant genome. Plant J 7:649–659
Altieri M (2000) The ecological impacts of transgenic crops on agroecosystem health. Ecosyst Health 6:13–23
Bayley CC, Morgan M, Dale EC, Ow D (1992) Exchange of gene activity in transgenic plants catalyzed by the Cre-lox site-specific recombination system. Plant Mol Biol 18:353–361
Belanger FC, Meagher TR, Day PR, Plumley K, Meyer WA (2003) Interspecific hybridization between Agrostis stolonifera and related Agrostis species under field conditions. Crop Sci 43:240–246
Boffey TB, Veevers A (1977) Balanced designs for two-component competition experiments. Euphytica 26:481–484
Bradford KJ, Van Deynze A, Gutterson N, Parrott W, Strauss SH (2005) Regulating transgenic crops sensibly: lessons from plant breeding, biotechnology and genomics. Nat Biotechnol 23:439–444
Dale EC, Ow DW (1990) Intra- and intermolecular site-specific recombination in plant cells mediated by bacteriophage P1 recombinase. Gene 91:79–85
Dale EC, Ow DW (1991) Gene transfer with subsequent removal of the selection gene from the host genome. Proc Natl Acad Sci USA 88:10558–10562
Dale PJ (1993) The release of transgenic plants into agriculture. J Agric Sci 120:1–5
Daniell H (2002) Molecular strategies for gene containment in transgenic crops. Nat Biotechnol 20:581–586
De Block M, Debrouwer D, Moens T (1997) The development of nuclear male sterility system in wheat: expression of the barnase gene under the control of tapetum specific promoters. Theor Appl Genet 95:125–131
De Cosa B, Moar W, Lee SB, Miller M, Daniell H (2001) Overexpression of the Bt cry2Aa2 operon in chloroplasts leads to formation of insecticidal crystals. Nat Biotechnol 19:71–74
Dunwell J, Ford CS (2005) Technologies for biological containment of GM and non-GM crops. DEFRA Contract CPEC 47, http://www.gmo-safety.eu/pdf/biosafenet/Defra_2005.pdf
Goetz M, Godt DE, Guivarc’h A, Kahmann U, Chriqui D, Roitsch T (2001) Induction of male sterility in plants by metabolic engineering of the carbohydrate supply. Proc Natl Acad Sci USA 98:6522–6527
Hanson DD, Hamilton DA, Travis JL, Bashe DM, Mascarenhas JP (1989) Characterization of a pollen-specific cDNA clone from Zea mays and its expression. Plant Cell 1:173–179
Hartley RW (1988) Barnase and Barstar: expression of its cloned inhibitor permits expression of a cloned ribonuclease. J Mol Biol 202:913–915
He S, Abad AR, Gelvin SB, Mackenzie SA (1996) A cytoplasmic male sterility-associate mitochondrial protein causes pollen disruption in transgenic tobacco. Proc Natl Acad Sci USA 93:11763–11768
Higginson T, Li SF, Parish RW (2003) AtMYB103 regulates tapetum and trichome development in Arabidopsis thaliana. Plant J 35:177–192
Jagannath A, Bandyopadhyay P, Arumugam N, Gupta V, Kumar P, Pental D (2001) The use of spacer DNA fragment insulates the tissue-specific expression of a cytotoxic gene (barnase) and allows high-frequency generation of transgenic male sterile lines in Brassica juncea L. Mol Breed 8:11–23
Lee J-Y, Aldemita RR, Hodges TK (1996) Isolation of a tapetum-specific gene and promoter from rice. Int Rice Res Newsl 21:2–3
Luo H, Lyznik LA, Gidoni D, Hodges TK (2000) FLP-mediated recombination for use in hybrid plant production. Plant J 23:423–430
Luo K, Duan H, Zhao D, Zheng X, Deng W, Chen Y, Stewart CN, McAvoy R, Jiang X, Wu Y, He A, Pei Y, Li Y (2007) "GM-gene-deletor": fused loxP-FRT recognition sequences dramatically improve the efficiency of FLP or CRE recombinase on transgene excision from pollen and seed of tobacco plants. Plant Biotechnol J 5:263–374
Mallory-Smith C, Zapiola ML (2008) Gene flow from glyphosate-resistant crops. Pest Manag Sci 64:428–440
Mariani C, Beuckeleer M, Truettner J, Leemans J, Goldberg R (1990) Induction of male sterility in plants by a chimaeric ribonuclease gene. Nature 374:737–738
Moffatt B, Somerville C (1988) Positive selection for male-sterile mutants of Arabidopsis lacking adenine phosphoribosyl transferase activity. Plant Physiol 86:1150–1154
Oliver MJ, Quisenberry JE, Trolinder N, Glover L, Keim DL (1998) Control of plant gene expression. US Patent 5723765. United States Patent and Trademarks Office, http://www.patentstorm.us/patents/5723765.html
Oliver MJ, Quisenberry JE, Trolinder N, Glover L, Keim DL (1999a) Control of plant gene expression. US Patent 5977441. United States Patent and Trademarks Office, http://www.patentstorm.us/patents/5977441.html
Oliver MJ, Quisenberry JE, Trolinder N, Glover L, Keim DL (1999b) Control of plant gene expression. US Patent 5925808. United States Patent and Trademarks Office, http://www.patentstorm.us/patents/5925808.html
Reichman JR, Watrud LS, Lee EH, Burdick CA, Bollman MA, Storm MJ, King GA, Mallory-Smith C (2006) Establishment of transgenic herbicide-resistant creeping bentgrass (Agrostis stolonifera L.) in nonagronomic habitats. Mol Ecol 15:4243–4255
Robertson GP, Dale VH, Doering OC, Hamburg SP, Melillo JM, Wander MM, Parton WJ, Adler PR, Barney JN, Cruse RM, Duke CS, Fearnside PM, Follett RF, Gibbs HK, Goldemberg J, Mladenoff DJ, Ojima D, Palmer MW, Sharpley A, Wallace L (2008) Sustainable biofuels redux. Science 322:49–50
Ruf S, Karcher D, Bock R (2007) Determining the transgene containment level provided by chloroplast transformation. Proc Natl Acad Sci USA 104:6998–7002
Snow AA, Moran Palma P (1997) Commercialization of transgenic plants: potential ecological risks. BioScience 47:86–96
Stegemann S, Hartmann S, Ruf S, Bock R (2003) High-frequency gene transfer from the chloroplast genome to the nucleus. Proc Natl Acad Sci USA 100:8828–8833
Stewart CN (2007) Biofuels and biocontainment. Nat Biotechnol 25:283–284
Sticklen MB (2008) Plant genetic engineering for biofuel production: towards affordable cellulosic ethanol. Nat Rev Genet 9:433–443
Theerakulpisut P, Xu H, Singh MB, Pettitt JM, Knox RB (1991) Isolation and developmental expression of Bcp1, an anther-specific cDNA clone in Brassica campestris. Plant Cell 3:1073–1084
Tomes DT, Huang B, Miller PD (1998) Genetic constructs and methods for producing fruits with very little or diminished seed. US Patent 5773697, http://www.freepatentsonline.com/5773697.html
Tsuchiya T, Toriyama K, Yoshikawa M, Ejiri S, Hinata K (1995) Tapetum-specific expression of the gene for an endo-beta-1,3-glucanase causes male sterility in transgenic tobacco. Plant Cell Physiol 36:487–494
Twell D, Wing R, Yamaguchi J, McCormick S (1989) Isolation and expression of an anther-specific gene from tomato. Mol Gen Genet 217:240–245
Watrud LS, Lee EH, Fairbrother A, Burdick C, Reichman JR, Bollman M, Storm M, King G, van De Waters, PK (2004) Evidence for landscape-level, pollen-mediated gene flow from genetically modified creeping bentgrass with CP4 EPSPS as a marker. Proc Natl Acad Sci USA 101:14533–14538
Wipff JK, Fricker C (2001) Gene flow from transgenic creeping bentgrass (Agrostis stolonifera L.) in the Willamette Valley, Oregon. Int Turfgrass Soc Res J 9:224–242
Xu H, Knox RB, Taylor PE, Singh MB (1995) Bcp1, a gene required for male fertility in Arabidopsis. Proc Natl Acad Sci USA 92:2106–2110
Yui R, Iketani S, Mikami T, Kubo T (2003) Antisense inhibition of mitochondrial pyruvate dehydrogenase E1α subunit in anther tapetum causes male sterility. Plant J 34:57–66
Zapiola ML, Campbell CK, Butler MD, Mallory-Smith CA (2008) Escape and establishment of transgenic glyphosate-resistant creeping bentgrass Agrostis stolonifera in Oregon, USA: a 4-year study. J Appl Ecol 45:486–494
Zapiola ML, Mallory-Smith CA, Thompson JH, Rue LJ, Campbell CK, Butler MD (2007) Gene escape from glyphosate-resistant creeping bentgrass fields: past, present, and future. Proceedings of the 60th Meeting of the Western Society of Weed Science, 13–15 March 2007, Portland, OR, Abstract 82
Zuo J, Chua NH (2000) Chemical-inducible systems for regulated expression of plant genes. Curr Opin Biotechnol 11:146–151
Zuo J, Niu QW, Chua NH (2000) An estrogen receptor-based transactivator XVE mediates highly inducible gene expression in transgenic plants. Plant J 24:265–273
Zou JT, Zhan XY, Wu HM, Wang H, Cheung AY (1994) Characterization of a rice pollen-specific gene and its expression. Am J Bot 81:552–561
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Kausch, A.P. et al. (2010). Genetic Modification in Dedicated Bioenergy Crops and Strategies for Gene Confinement. In: Mascia, P., Scheffran, J., Widholm, J. (eds) Plant Biotechnology for Sustainable Production of Energy and Co-products. Biotechnology in Agriculture and Forestry, vol 66. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-13440-1_11
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DOI: https://doi.org/10.1007/978-3-642-13440-1_11
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