Skip to main content

Caspase-3

  • Chapter
Class 3 Hydrolases

Part of the book series: Springer Handbook of Enzymes ((HDBKENZYMES,volume S6))

  • 673 Accesses

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 259.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 329.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Srinivasula, S.M.; Fernandes-Alnemri, T.; Zangrilli, J.; Robertson, N.; Armstrong, R.C.; Wang, L.; Trapani, J.A.; Tomaselli, K.J.; Litwack, G.; Alnemri E.S.: The Ced-3/interleukin 1β converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2α are substrates for the apoptotic mediator CPP32. J. Biol. Chem., 271, 27099–27106 (1996)

    Article  PubMed  CAS  Google Scholar 

  2. Zhivotovsky, B.; Samali, A.; Gahm, A.; Orrenius, S.: Caspases: their intracellular localization and translocation during apoptosis. Cell Death Differ., 6, 644–651 (1999)

    Article  PubMed  CAS  Google Scholar 

  3. Nakajima, K.; Takahashi, A.; Yaoita, Y.: Structure, expression, and function of the Xenopus laevis caspase family. J. Biol. Chem., 275, 10484–10491 (2000)

    Article  PubMed  CAS  Google Scholar 

  4. Garcia-Calvo, M.; Peterson, E.P.; Leiting, B.; Ruel, R.; Nicholson, D.W.; Thornberry, N.A.: Inhibition of human caspases by peptide-based and macromolecular inhibitors. J. Biol. Chem., 273, 32608–32613 (1998)

    Article  PubMed  CAS  Google Scholar 

  5. Garcia-Calvo, M.; Peterson, E.P.; Rasper, D.M.; Vaillancourt, J.P.; Zamboni, R.; Nicholson, D.W.; Thornberry, N.A.: Purification and catalytic properties of human caspase family members. Cell Death Differ., 6, 362–369 (1999)

    Article  PubMed  CAS  Google Scholar 

  6. Chang, H.Y.; Yang, X.: Proteases from cell suicide: functions and regulation of caspases. Microbiol. Mol. Biol. Rev., 64, 821–846 (2000)

    Article  PubMed  CAS  Google Scholar 

  7. Thornberry, N.A.; Rano, T.A.; Peterson, E.P.; et al.: A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis. J. Biol. Chem., 272, 17907–17911 (1997)

    Article  PubMed  CAS  Google Scholar 

  8. Fernandes-Alnemri, T.; Armstrong, R.C.; Krebs, J.F.; Srinivasula, S.M.; Wang, L.; Bullrich, F.; Fritz, L.C.; Trapani, J.A.; Tomaselli, K.J.; Litwack, G.; Alnemri, E.S.: In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains. Proc. Natl. Acad. Sci. USA, 93, 7464–7469 (1996)

    Article  PubMed  CAS  Google Scholar 

  9. van de Graen, M.; Berx, G.; van den Brande, I.; Fiers, W.; Declercq, W.; Vandenabeele, P.: Proteolytic cleavage of β-catenin by caspases: an in vitro analysis. FEBS Lett., 458, 167–170 (1999)

    Article  Google Scholar 

  10. van de Craen, M.; Vandenabeele, P.; Declercq, W.; van den Brande, I.; van Loo, G.; Molemans, F.; Schotte, P.; van Criekinge, W.; Beyaert, R.; Fiers, W.: Characterization of seven murine caspase family members. FEBS Lett., 403, 61–69 (1997)

    Article  PubMed  Google Scholar 

  11. Talanian, R.V.; Quinlan, C.; Trautz, S.; Hackett, M.C.; Mankovich, J.A.; Banach, D.; Ghayur, T.; Brady, K.D.; Wong, W.W.: Substrate specificities of caspase family proteases. J. Biol. Chem., 272, 9677–9682 (1997)

    Article  PubMed  CAS  Google Scholar 

  12. Samejima, K.; Svigen, P.A.; Basi, G.S.; Kottke, T.; Mesner, P.W.; Stewart, L.; Durrieu, F.; Poirier, G.G.; Alnemri, E.S.; Champoux, J.J.; Kaufmann, S.H.; Earnshaw, W.C.: caspase-mediated cleavage of DNA topoisomerase I at unconventional sites during apoptosis. J. Biol. Chem., 274, 4335–4340 (1999)

    Article  PubMed  CAS  Google Scholar 

  13. Hirata, H.; Takahashi, A.; Kobayashi, S.; Yonehara, S.; Sawai, H.; Okazaki, T.; Yamamoto, K.; Sasada, M.: Caspases are activated in a branched protease cascade and control didtinct downstream processes in fas-induced apoptosis. J. Exp. Med., 187, 587–600 (1998)

    Article  PubMed  CAS  Google Scholar 

  14. Fernandes-Alnemri, T.; Litwack, G.; Alnemri E.S.: CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 β-converting enzyme. J. Biol. Chem., 269, 30761–30764 (1994)

    PubMed  CAS  Google Scholar 

  15. Tewari, M.; Quan, L.T.; O’Rourke, K.; Desnoyers, S.; Zeng, Z.; Beidler, D.R.; Poirier, G.G.; Salvesen, G.S.; Dixit, V.M.: Yama/CPP32 β, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase. Cell, 81, 801–809 (1995)

    Article  PubMed  CAS  Google Scholar 

  16. Lee, D.; Long, S.A.; Adams, J.L.; Chan, G.; et al.: Potent and selective nonpeptide inhibitors of caspases 3 and 7 inhibit apoptosis and maintain cell functionality. J. Biol. Chem., 275, 16007–16014 (2000)

    Article  PubMed  CAS  Google Scholar 

  17. Goldberg, Y.P.; Nicholson, D.W.; Rasper, D.M.; Kalchman, M.A.; Koide, H.B.; et al.: Cleavage of huntingtin by apopain, a proapoptotic cysteine protease, is modulated by the polyglutamine tract. Nat. Genet., 13, 442–449 (1996)

    Article  PubMed  CAS  Google Scholar 

  18. Mukasa, T.; Urase, K.; Momoi, M.Y.; Kimura, I.; Momoi, T.: Specific expression of CPP32 in sensory neurons of mouse embryos and activation of CPP32 in the apoptosis induced by a withdrawal of NGG. Biochem. Biophys. Res. Commun., 231, 770–774 (1997)

    Article  PubMed  CAS  Google Scholar 

  19. Flaws, J.A.; Kugu, K.; Trbovich, A.M.; Desanti, A.; Tilly, K.I.; Hirshfield, A.N.; Tilly, J.L.: Interleukin-1 β-converting enzyme-related proteases (IRPs) and mammalian cell death: dissociation of IRP-induced oligonucleosomal endonuclease activity from morphological apoptosis in granulosa cells of the ovarian follicle. Endocrinology, 136, 5042–5053 (1995)

    Article  PubMed  CAS  Google Scholar 

  20. Ni, B.; Wu, X.; Du, Y.; Su, Y.; Hamilton-Byrd, E.; Rockey, P.K.; Rosteck, P. Jr.; Poirier, G.G.; Paul, S.M.: Cloning and expression of a rat brain interleukin-1 β-converting enzyme (ICE)-related protease (IRP) and its possible role in apoptosis of cultured cerebellar granule neurons. J. Neurosci., 17, 1561–1569 (1997)

    PubMed  CAS  Google Scholar 

  21. Yaoita, Y.; Nakajima, K.: Induction of apoptosis and CPP32 expression by thyroid hormone in a myoblastic cell line derived from tadpole tail. J. Biol. Chem., 272, 5122–5127 (1997)

    Article  PubMed  CAS  Google Scholar 

  22. Bellido, T.; Huening, M.; Raval-Pandya, M.; Manolagas, S.C.; Christakos, S.: Calbindin-D28k is expressed in osteoblastic cells and suppresses their apoptosis by inhibiting caspase-3 activity. J. Biol. Chem., 275, 26328–26332 (2000)

    Article  PubMed  CAS  Google Scholar 

  23. Decker, P.; Isenberg, D.; Muller, S.: Inhibition of caspase-3-mediated poly-(ADP-ribose) polymerase (PARP) apoptotic cleavage by human PARP autoantibodies and effect on cells undergoing apoptosis. J. Biol. Chem., 275, 9043–9046 (2000)

    Article  PubMed  CAS  Google Scholar 

  24. Djerbi, M.; Darreh-Shori, T.; Zhivotovsky, B.; Grandien, A.: Characterization of the human FLICE-inhibitory protein locus and comparison of the anti-apoptotic activity of four different FLIP isoforms. Scand. J. Immunol., 54, 180–189 (2001)

    Article  PubMed  CAS  Google Scholar 

  25. Mohr, S.; Zech, B.; Lapetina, E.G.; Brune, B.: Inhibition of caspase-3 by Snitrosation and oxidation caused by nitric oxide. Biochem. Biophys. Res. Commun., 238, 387–391 (1997)

    Article  PubMed  CAS  Google Scholar 

  26. Guo, Z.; Xian, M.; Zhang, W.; McGill, A.; Wang, P.G.: N-nitrosoanilines: a new class of caspase-3 inhibitors. Bioorg. Med. Chem., 9, 99–106 (2001)

    Article  PubMed  CAS  Google Scholar 

  27. Gottlob, K.; Fulco, M.; Levrero, M.; Graessmann, A.: The hepatitis B virus HBx protein inhibits caspase 3 activity. J. Biol. Chem., 273, 33347–33353 (1998)

    Article  PubMed  CAS  Google Scholar 

  28. Han, B.H.; Xu, D.; Choi, J.; Han, Y.; Xanthoudakis, S.; Roy, S.; Tam, J.; Vaillancourt, J.; Colucci, J.; Siman, R.; Giroux, A.; Robertson, G.S.; Zamboni, R.; Nicholson, D.W.; Holtzman, D.M.: Selective, reversible caspase-3 inhibitor is neuroprotective and reveals distinct pathways of cell death after neonatal hypoxic-ischemic brain injury. J. Biol. Chem., 277, 30128–30136 (2002)

    Article  PubMed  CAS  Google Scholar 

  29. Erhadt, P.; Tomaselli, K.J.; Cooper, G.M.: Identification of the MDM2 oncoprotein as a substrate for CPP32-like apoptotic proteases. J. Biol. Chem., 272, 15049–15052 (1997)

    Article  Google Scholar 

  30. Krebs, J.F.; Srinivasan, A.; Wong, A.M.; Tomaselli, K.J.; Fritz, L.C.; Wu, J.C.: Heavy membrane-associated caspase 3: identification, isolation, and characterization. Biochemistry, 39, 16056–16063 (2000)

    Article  PubMed  CAS  Google Scholar 

  31. Marissen, W.E.; Lloyd, R.E.: Eukaryotic translation initiation factor 4G is targeted for proteolytic cleavage by caspase 3 during inhibition of translation in apoptotic cells. Mol. Cell. Biol., 18, 7565–7574 (1998)

    PubMed  CAS  Google Scholar 

  32. Rheaume, E.; Cohen, L.Y.; Uhlmann, F.; Lazure, C.; Alam, A.; Hurwitz, J.; Sekaly, R.-P.; Denis, F.: The large subunit of replication factor C is a substrate for caspase-3 in vitro and is cleaved by a caspase-3-like protease during Fas-mediated apoptosis. EMBO J., 16, 6346–6354 (1997)

    Article  PubMed  CAS  Google Scholar 

  33. Rössig, L.; Fichtlscherer, B.; Breitschopf, K.; Haendeler, J.; Zeiher, A.M.; Mulsch, A.; Dimmeler, S.: Nitric oxide inhibits caspase-3 by S-nitrosation in vivo. J. Biol. Chem., 274, 6823–6826 (1999)

    Article  PubMed  Google Scholar 

  34. Schlegel, J.; Peters, I.; Orrenius, S.; Miller, D.K.; Thornberry, N.A.; Yamin, T.-T.; Nicholson, D.W.: CPP32/apopain is a key interleukin 1β converting enzyme-like protease involved in Fas-mediated apoptosis. J. Biol. Chem., 271, 1841–1844 (1996)

    Article  PubMed  CAS  Google Scholar 

  35. Roy, N.; Deveraux, Q.L.; Takahashi, R.; Salvesen, G.S.; Reed, J.C.: The c-IAP-1 and c-IAP-2 proteins are direct inhibitors of specific caspases. EMBO J., 16, 6914–6925 (1997)

    Article  PubMed  CAS  Google Scholar 

  36. Shin, S.; Sung, B.J.; Cho, Y.S.; Kim, H.J.; Ha, N.C.; Hwang, J.I.; Chung, C.W.; Jung, Y.K.; Oh, B.H.: An anti-apoptotic protein human survivin is a direct inhibitor of caspase-3 and−7. Biochemistry, 40, 1117–1123 (2001)

    Article  PubMed  CAS  Google Scholar 

  37. Smith, L.; Chen, L.; Reyland, M.E.; DeVries, T.A.; Talanian, R.V.; Omura, S.; Smith, J.B.: Activation of atypical protein kinase C σ by caspase processing and degradation by the ubiquitin-proteasome system. J. Biol. Chem., 275, 40620–40627 (2000)

    Article  PubMed  CAS  Google Scholar 

  38. Takahashi, A.; Alnemri, E.S.; Lazebnik, Y.A.; Fernandes-Alnemri, T.; Litwack, G.; Moir, R.D.; Goldman, R.D.; Poirier, G.G.; Kaufmann, S.H.; Earnshaw, W.C.: Cleavage of lamin A by Mch2 α but not CPP32: multiple interleukin 1 β-converting enzyme-related proteases with distinct substrate recognition properties are active in apoptosis. Proc. Natl. Acad. Sci. USA, 93, 8395–8400 (1996)

    Article  PubMed  CAS  Google Scholar 

  39. Yabu, T.; Kishi, S.; Okazaki, T.; Yamashita, M.: Characterization of zebrafish caspase-3 and induction of apoptosis through ceramide generation in fish fathead minnow tailbud cells and zebrafish embryo. Biochem. J., 360, 39–47 (2001)

    Article  PubMed  CAS  Google Scholar 

  40. Choong, I.C.; Lew, W.; Lee, D.; Pham, P.; Burdett, M.T.; Lam, J.W.; Wiesmann, C.; Luong, T.N.; Fahr, B.; DeLano, W.L.; McDowell, R.S.; Allen, D.A.; Erlanson, D.A.; Gordon, E.M.; O’Brien, T.: Identification of potent and selective small-molecule inhibitors of caspase-3 through the use of extended tethering and structure-based drug design. J. Med. Chem., 45, 5005–5022 (2002)

    Article  PubMed  CAS  Google Scholar 

  41. Essmann, F.; Wieder, T.; Otto, A.; Muller, E.C.; Dorken, B.; Daniel, P.T.: GDP dissociation inhibitor D4-GDI (Rho-GDI 2), but not the homologous rho-GDI 1, is cleaved by caspase-3 during drug-induced apoptosis. Biochem. J., 346, 777–783 (2000)

    Article  PubMed  CAS  Google Scholar 

  42. Perry, D.K.; Smyth, M.J.; Stennicke, H.R.; Salvesen, G.S.; Duriez, P.; Poirier, G.G.; Hannun, Y.A.: Zinc is a potent inhibitor of the apoptotic protease, caspase-3. A novel target for zinc in the inhibition of apoptosis. J. Biol. Chem., 272, 18530–18533 (1997)

    Article  PubMed  CAS  Google Scholar 

  43. Deveraux, Q.L.; Roy, N.; Stennicke, H.R.; Van Arsdale, T.; Zhou, Q.; Srinivasula, S.M.; Alnemri, E.S.; Salvesen, G.S.; Reed, J.C.: IAPs block apoptotic events induced by caspase-8 and cytochrome c by direct inhibition of distinct caspases. EMBO J., 17, 2215–2223 (1998)

    Article  PubMed  CAS  Google Scholar 

  44. Johnson, A.L.; Bridgeham, J.T.: Caspase-3 and −6 expression and enzyme activity in hen granulosa cell. Biol. Reprod., 62, 589–598 (2000)

    Article  PubMed  CAS  Google Scholar 

  45. Mittl, P.R.E.; di Marco, S.; Krebs, J.F.; et al.: Structure of recombinant human CPP32 in complex with the tetrapeptide acetyl-Asp-Val-Ala-Asp fluoromethyl ketone. J. Biol. Chem., 272, 6539–6547 (1997)

    Article  PubMed  CAS  Google Scholar 

  46. Margolin, N.; Raybuck, S.A.; Wilson, K.P.; Chen, W.; Fox, T.; Gu, Y.; Livingston, D.J.: Substrate and inhibitor specificity of interleukin-1β-converting enzyme and related caspases. J. Biol. Chem., 272, 7223–7228 (1997)

    Article  PubMed  CAS  Google Scholar 

  47. Pirhonen, J.; Sareneva, T.; Julkunen, I.; Matikainen, S.: Virus infection induces proteolytic processing of IL-18 in human macrophages via caspase-1 and caspase-3 activation. Eur. J. Immunol., 31, 726–733 (2001)

    Article  PubMed  CAS  Google Scholar 

  48. Bae, S.S.; Choi, J.H.; Oh, Y.S.; Perry, D.K.; Ryu, S.H.; Suh, P.G.: Proteolytic cleavage of epidermal growth factor receptor by caspases. FEBS Lett., 491, 16–20 (2001)

    Article  PubMed  CAS  Google Scholar 

  49. Kisselev, A.F.; Garcia-Calvo, M.; Overkleeft, H.S.; Peterson, E.; Pennington, M.W.; Ploegh, H.L.; Thornberry, N.A.; Goldberg, A.L.: the caspase-like sites of proteasomes, their substrate specificity, new inhibitors and substrates, and allosteric interactions with the trypsin-like sites. J. Biol. Chem., 278, 35869–35877 (2003)

    Article  PubMed  CAS  Google Scholar 

  50. Tawa, P.; Giroux, A.; Grimm, E.; Han, Y.; Nicholson, D.W.; Xanthoudakis, S.: Correlating the fractional inhibition of caspase-3 in NT2 cells with apoptotic markers using an active-caspase-3 enzyme-linked immunosorbent assay. Anal. Biochem., 350, 32–40 (2006)

    Article  PubMed  CAS  Google Scholar 

  51. Han, Y.; Giroux, A.; Colucci, J.; Bayly, C.I.; Mckay, D.J.; Roy, S.; Xanthoudakis, S.; Vaillancourt, J.; Rasper, D.M.; Tam, J.; Tawa, P.; Nicholson, D.W.; Zamboni, R.J.: Novel pyrazinone mono-amides as potent and reversible caspase-3 inhibitors. Bioorg. Med. Chem. Lett., 15, 1173–1180 (2005)

    Article  PubMed  CAS  Google Scholar 

  52. Wang, Z.Q.; Liao, J.; Diwu, Z.: N-DEVD-N-morpholinecarbonyl-rhodamine 110: novel caspase-3 fluorogenic substrates for cell-based apoptosis assay. Bioorg. Med. Chem. Lett., 15, 2335–2338 (2005)

    Article  PubMed  CAS  Google Scholar 

  53. Zhang, Y.; Bhavnani, B.R.: Glutamate-induced apoptosis in neuronal cells is mediated via caspase-dependent and independent mechanisms involving calpain and caspase-3 proteases as well as apoptosis inducing factor (AIF) and this process is inhibited by equine estrogens. BMC Neurosci., 7, 49 (2006)

    Article  PubMed  CAS  Google Scholar 

  54. Schlittenhardt, D.; Schmiedt, W.; Bonaterra, G.A.; Metz, J.; Kinscherf, R.: Colocalization of oxidized low-density lipoprotein, caspase-3, cyclooxygenase-2, and macrophage migration inhibitory factor in arteriosclerotic human carotid arteries. Cell Tissue Res., 322, 425–435 (2005)

    Article  PubMed  CAS  Google Scholar 

  55. Kravchenko, D.V.; Kysil, V.M.; Tkachenko, S.E.; Maliarchouk, S.; Okun, I.M.; Ivachtchenko, A.V.: Pyrrolo [3,4-c] quinoline-1,3-diones as potent caspase-3 inhibitors. Synthesis and SAR of 2-substituted 4-methyl-8-(morpholine-4-sulfonyl)-pyrrolo [3,4-c] quinoline-1,3-diones. Eur. J. Med. Chem., 40, 1377–1383 (2005)

    Article  PubMed  CAS  Google Scholar 

  56. Sliskovic, I.; Mutus, B.: Reversible inhibition of caspase-3 activity by iron(III): potential role in physiological control of apoptosis. FEBS Lett., 580, 2233–2237 (2006)

    Article  PubMed  CAS  Google Scholar 

  57. Burkhardt, B.R.; Greene, S.R.; White, P.; Wong, R.K.; Brestelli, J.E.; Yang, J.; Robert, C.E.; Brusko, T.M.; Wasserfall, C.H.; Wu, J.; Atkinson, M.A.; Gao, Z.; Kaestner, K.H.; Wolf, B.A.: PANDER-induced cell-death genetic networks in islets reveal central role for caspase-3 and cyclin-dependent kinase inhibitor 1A (p21). Gene, 369, 134–141 (2006)

    Article  PubMed  CAS  Google Scholar 

  58. Weng, C.; Li, Y.; Xu, D.; Shi, Y.; Tang, H.: Specific cleavage of Mcl-1 by caspase-3 in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in Jurkat leukemia T cells. J. Biol. Chem., 280, 10491–10500 (2005)

    Article  PubMed  CAS  Google Scholar 

  59. Ikun, I.; Malarchuk, S.; Dubrovskaya, E.; Khvat, A.; Tkachenko, S.; Kysil, V.; Ilyin, A.; Kravchenko, D.; Prossnitz, E.R.; Sklar, L.; Ivachtchenko, A.: Screening for caspase-3 inhibitors: a new class of potent small-molecule inhibitors of caspase-3. J. Biomol. Screen., 11, 277–285 (2006)

    Article  CAS  Google Scholar 

  60. Faraglia, -B.; Bonsignore, A.; Scaldaferri, F.; Boninsegna, A.; Cittadini, A.; Mancuso, C.; Sgambato, A.: Caspase-3 inhibits the growth of breast cancer cells independent of protease activity. J. Cell. Physiol., 202, 478–482 (2005)

    Article  PubMed  CAS  Google Scholar 

  61. Clements, K.M.; Burton-Wurster, N.; Nuttall, M.E.; Lust, G.: Caspase-3/7 inhibition alters cell morphology in mitomycin-C treated chondrocytes. J. Cell. Physiol., 205, 133–140 (2005)

    Article  PubMed  CAS  Google Scholar 

  62. Chen, Y.H.; Zhang, Y.H.; Zhang, H.J.; Liu, D.Z.; Gu, M.; Li, J.Y.; Wu, F.; Zhu, X.Z.; Li, J.; Nan, F.J.: Design, synthesis, and biological evaluation of isoquinoline-1,3,4-trione derivatives as potent caspase-3 inhibitors. J. Med. Chem., 49, 1613–1623 (2006)

    Article  PubMed  CAS  Google Scholar 

  63. Ganesan, R.; Mittl, P.R.; Jelakovic, S.; Gruetter, M.G.: Extended substrate recognition in caspase-3 revealed by high resolution X-ray structure analysis. J. Mol. Biol., 359, 1378–1388 (2006)

    Article  PubMed  CAS  Google Scholar 

  64. Fang, B.; Boross, P.I.; Tozser, J.; Weber, I.T.: Structural and kinetic analysis of caspase-3 reveals role for S5 binding site in substrate recognition. J. Mol. Biol., 360, 654–666 (2006)

    Article  PubMed  CAS  Google Scholar 

  65. Sano, J.; Oguma, K.; Kano, R.; Hasegawa, A.: Characterization of canine caspase-3. J. Vet. Med. Sci., 66, 563–567 (2004)

    Article  PubMed  CAS  Google Scholar 

  66. Goode, D.R.; Sharma, A.K.; Hergenrother, P.J.: Using peptidic inhibitors to systematically probe the S1 site of caspase-3 and caspase-7. Org. Lett., 7, 3529–3532 (2005)

    Article  PubMed  CAS  Google Scholar 

  67. Chakraborty, C.; Nandi, S.S.; Sinha, S.; Gera, V.K.: Zebrafish caspase-3: molecular cloning, characterization, crystallization and phylogenetic analysis. Protein Pept. Lett., 13, 633–640 (2006)

    Article  PubMed  CAS  Google Scholar 

Download references

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2009 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

(2009). Caspase-3. In: Chang, A. (eds) Class 3 Hydrolases. Springer Handbook of Enzymes, vol S6. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-85705-1_10

Download citation

Publish with us

Policies and ethics