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PCR Amplification and Sequence Analysis of GC-Rich Sequences: Aristaless-Related Homeobox Example

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Tandem Repeats in Genes, Proteins, and Disease

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1017))

Abstract

PCR amplification (followed by mutation scanning or direct sequencing) is a technique widely used in mutation detection and molecular studies of disease-causing genes, such as ARX. PCR amplification of high GC-rich regions encounters difficulties using conventional PCR procedures. Here, we present the strategies to amplify and sequence these GC-rich regions for the purposes of mutation screening and other molecular analyses.

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Acknowledgments

This work was supported by grants from the National Health and Medical Research Council of Australia (Project Grant 1002732 to C.S.; Principle Research Fellowship 508043 to J.G.) and MS McLeod Foundation Fellowship to C.S. and postgraduate scholarship to M.H.T.

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Authors and Affiliations

  1. Department of Paediatrics, University of Adelaide, Adelaide, SA, Australia

    May H. Tan, Jozef Gécz & Cheryl Shoubridge

  2. Neurogenetics, Department of Genetic and Molecular Pathology, SA Pathology, Adelaide, SA, Australia

    Jozef Gécz

Authors
  1. May H. Tan
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  2. Jozef Gécz
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  3. Cheryl Shoubridge
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Editor information

Editors and Affiliations

  1. , Dept. of Biochemistry and Molecular Biol, The University of Melbourne, Flemington Road 30, Parkville, 3010, Victoria, Australia

    Danny M. Hatters

  2. Florey Neuroscience Institutes, and Mental Health, The University of Melbourne, Royal Parade, Parkville, 3010, Victoria, Australia

    Anthony J. Hannan

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Tan, M.H., Gécz, J., Shoubridge, C. (2013). PCR Amplification and Sequence Analysis of GC-Rich Sequences: Aristaless-Related Homeobox Example. In: Hatters, D., Hannan, A. (eds) Tandem Repeats in Genes, Proteins, and Disease. Methods in Molecular Biology, vol 1017. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-438-8_8

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  • DOI: https://doi.org/10.1007/978-1-62703-438-8_8

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-437-1

  • Online ISBN: 978-1-62703-438-8

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