Abstract
Peptide microarray technology can be used to identify substrates for recombinant kinases, to measure kinase activity and changes thereof in cell lysates and lysates from fresh frozen (tumor) tissue. The effect of kinase inhibitors on the kinase activities in relevant tissues can be investigated as well. The method for performing experiments on dynamic peptide microarrays with real-time readout is described, as well as the influence of assay parameters and suggestions for optimization of experiments.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Thiele A, Stangl GI, Schutkowski M (2011) Deciphering enzyme function using peptide arrays. Mol Biotechnol 49:283–305
Arsenault R, Griebel P, Napper S (2011) Peptide arrays for kinome analysis: new opportunities and remaining challenges. Proteomics 11:4595–4609
Lemeer S, Jopling C, Naji F et al (2007) Protein-tyrosine kinase activity profiling in knock down zebrafish embryos. PLoS One 2:e581
Versele M, Talloen W, Rockx C et al (2009) Response prediction to a multitargeted kinase inhibitor in cancer cell lines and xenograft tumors using high-content tyrosine peptide arrays with a kinetic readout. Mol Cancer Ther 8:1846–1855
Hilhorst R, Houkes L, van den Berg A et al (2009) Peptide microarrays for detailed, high-throughput substrate identification, kinetic characterization, and inhibition studies on protein kinase A. Anal Biochem 387:150–161
Sanz A, Ungureanu D, Pekkala T et al (2011) Analysis of Jak2 catalytic function by peptide microarrays: the role of the JH2 domain and V617F mutation. PLoS One 6:e18522
Poot AJ, van Ameijde J, Slijper M et al (2009) Development of selective bisubstrate-based inhibitors against protein kinase C (PKC) isozymes by using dynamic peptide microarrays. Chembiochem 10:2042–2051
Harmsen S, Dolman ME, Nemes Z et al (2011) Development of a cell-selective and intrinsically active multikinase inhibitor bioconjugate. Bioconjug Chem 22:540–545
Sikkema AH, Diks SH, den Dunnen WF et al (2009) Kinome profiling in pediatric brain tumors as a new approach for target discovery. Cancer Res 69:5987–5995
Bratland A, Boender PJ, Hoifodt HK et al (2009) Osteoblast-induced EGFR/ERBB2 signaling in androgen-sensitive prostate carcinoma cells characterized by multiplex kinase activity profiling. Clin Exp Metastasis 26: 485–496
Plaza-Menacho I, Morandi A, Mologni L et al (2011) Focal adhesion kinase (FAK) binds RET kinase via its FERM domain, priming a direct and reciprocal RET-FAK transactivation mechanism. J Biol Chem 286:17292–17302
Maat W, el Filali M, Dirks-Mulder A et al (2009) Episodic Src activation in uveal melanoma revealed by kinase activity profiling. Br J Cancer 101:312–319
Saelen MG, Flatmark K, Folkvord S et al (2011) Tumor kinase activity in locally advanced rectal cancer: angiogenic signaling and early systemic dissemination. Angiogenesis 14:481–489
Ter Elst A, Diks SH, Kampen KR et al (2011) Identification of new possible targets for leukemia treatment by kinase activity profiling. Leuk Lymphoma 52:122–130
Jinnin M, Medici D, Park L et al (2008) Suppressed NFAT-dependent VEGFR1 expression and constitutive VEGFR2 signaling in infantile hemangioma. Nat Med 14: 1236–1246
Folkvord S, Flatmark K, Dueland S et al (2010) Prediction of response to preoperative chemoradiotherapy in rectal cancer by multiplex kinase activity profiling. Int J Radiat Oncol Biol Phys 78:555–562
Hilhorst R, Schaake E, van Pel R et al (2011) Application of kinase activity profiles to predict response to erlotinib in a neoadjuvant setting in early stage non-small cell lung cancer (NSCLC). J Clin Oncol 28: suppl. abstract 10566
Hilhorst R, Schaake E., van Pel R et al (2011) Blind prediction of response to erlotinib in early stage non-small cell lung cancer (NSCLC) in a neoadjuvant settin based on kinase activity profiles. J Clin Oncol 29: suppl abstract 10521
Lemeer S, Ruijtenbeek R, Pinkse MW et al (2007) Endogenous phosphotyrosine signaling in zebrafish embryos. Mol Cell Proteomics 6:2088–2099
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Hilhorst, R., Houkes, L., Mommersteeg, M., Musch, J., van den Berg, A., Ruijtenbeek, R. (2013). Peptide Microarrays for Profiling of Serine/Threonine Kinase Activity of Recombinant Kinases and Lysates of Cells and Tissue Samples. In: Bina, M. (eds) Gene Regulation. Methods in Molecular Biology, vol 977. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-284-1_21
Download citation
DOI: https://doi.org/10.1007/978-1-62703-284-1_21
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-283-4
Online ISBN: 978-1-62703-284-1
eBook Packages: Springer Protocols