Abstract
C. elegans has been widely used as a model organism for basic biological research and is particularly amenable for molecular genetic studies using a broad repertoire of techniques. Biochemical approaches, including Western blot analysis, have emerged as a powerful tool in C. elegans biology for understanding molecular mechanisms that link genotypes to phenotypes. Here, we provide a protocol for Western blot analysis using protein extracts obtained from C. elegans samples.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
 References
Corsi AK, Wightman B, Chalfie M (2015) A transparent window into biology: a primer on Caenorhabditis elegans. Genetics 200:387–407
Hutter H (2012) Fluorescent protein methods: strategies and applications. Methods Cell Biol 107:67–92
Evans TC (2005) Transformation and microinjection. WormBook:1–15
Praitis V, Maduro MF (2011) Transgenesis in C. elegans. Methods Cell Biol 106:161–185
Frokjaer-Jensen C, Davis MW, Hopkins CE, Newman BJ, Thummel JM, Olesen SP, Grunnet M, Jorgensen EM (2008) Single-copy insertion of transgenes in Caenorhabditis elegans. Nat Genet 40:1375–1383
Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella M, Pozniakovsky A, Moerman DG, Jorgensen EM (2014) Random and targeted transgene insertion in Caenorhabditis elegans using a modified Mos1 transposon. Nat Methods 11:529–534
Dickinson DJ, Goldstein B (2016) CRISPR-based methods for Caenorhabditis elegans genome engineering. Genetics 202:885–901
Kim HM, Colaiacovo MP (2016) CRISPR-Cas9-guided genome engineering in C. elegans. Curr Protoc Mol Biol 115:31.37.1–31.37.18
Duerr JS (2006) Immunohistochemistry. WormBook:1–61
Hadwiger G, Dour S, Arur S, Fox P, Nonet ML (2010) A monoclonal antibody toolkit for C. elegans. PLoS One 5:e10161
Arur S, Schedl T (2014) Generation and purification of highly specific antibodies for detecting post-translationally modified proteins in vivo. Nat Protoc 9:375–395
Fonslow BR, Moresco JJ, Tu PG, Aalto AP, Pasquinelli AE, Dillin AG, Yates JR 3rd (2014) Mass spectrometry-based shotgun proteomic analysis of C. elegans protein complexes. WormBook:1–18
Walhout AJ (2006) Biochemistry and molecular biology. WormBook:1–10
Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
Stiernagle T (2006) Maintenance of C. elegans. WormBook:1–11
Krohn RI (2011) The colorimetric detection and quantitation of total protein. Curr Protoc Cell Biol Appendix 3:3H
Acknowledgments
This work was supported by the Science Research Center grant (NRF-2017R1A5A1015366) funded by the Ministry of Science, ICT, and Future Planning of South Korea to S.-J.V.L.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Jeong, DE., Lee, Y., Lee, SJ.V. (2018). Western Blot Analysis of C. elegans Proteins. In: Huang, L. (eds) Hypoxia. Methods in Molecular Biology, vol 1742. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7665-2_19
Download citation
DOI: https://doi.org/10.1007/978-1-4939-7665-2_19
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7664-5
Online ISBN: 978-1-4939-7665-2
eBook Packages: Springer Protocols