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Western Blot Analysis of C. elegans Proteins

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Hypoxia

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1742))

Abstract

C. elegans has been widely used as a model organism for basic biological research and is particularly amenable for molecular genetic studies using a broad repertoire of techniques. Biochemical approaches, including Western blot analysis, have emerged as a powerful tool in C. elegans biology for understanding molecular mechanisms that link genotypes to phenotypes. Here, we provide a protocol for Western blot analysis using protein extracts obtained from C. elegans samples.

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Acknowledgments

This work was supported by the Science Research Center grant (NRF-2017R1A5A1015366) funded by the Ministry of Science, ICT, and Future Planning of South Korea to S.-J.V.L.

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Correspondence to Seung-Jae V. Lee .

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Jeong, DE., Lee, Y., Lee, SJ.V. (2018). Western Blot Analysis of C. elegans Proteins. In: Huang, L. (eds) Hypoxia. Methods in Molecular Biology, vol 1742. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7665-2_19

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  • DOI: https://doi.org/10.1007/978-1-4939-7665-2_19

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7664-5

  • Online ISBN: 978-1-4939-7665-2

  • eBook Packages: Springer Protocols

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