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Development of Lentiviral Vectors for Targeted Integration and Protein Delivery

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Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1448))

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Abstract

The method in this chapter describes the design of human immunodeficiency virus type 1 (HIV-1) integrase (IN)-fusion proteins which we have developed to transport different proteins into the nuclei of lentiviral vector (LV)-transduced cells. The IN-fusion protein cDNA is incorporated into the LV packaging plasmid, which leads to its incorporation into vector particles as part of a large Gag–Pol polyprotein. This specific feature of protein packaging enables also the incorporation of cytotoxic and proapoptotic proteins, such as frequently cutting endonucleases and P53. The vectors can hence be used for various protein transduction needs. An outline of the necessary methods is also given to study the functionality of a chosen IN-fusion protein in a cell culture assay.

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Acknowledgments

This work was supported by grants from Finnish Academy, ERC, the Sigrid Juselius Foundation, the Eemil Aaltonen Foundation, the Instrumentarium Science Foundation, and the Ella and Georg Ehrnrooth Foundation.

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Authors and Affiliations

  1. Department of Biotechnology and Molecular Medicine, A.I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio, Finland

    Diana Schenkwein & Seppo Ylä-Herttuala

Authors
  1. Diana Schenkwein
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  2. Seppo Ylä-Herttuala
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Corresponding author

Correspondence to Seppo Ylä-Herttuala .

Editor information

Editors and Affiliations

  1. Istituto Superiore di Sanità, National AIDS Center, Rome, Italy

    Maurizio Federico

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Schenkwein, D., Ylä-Herttuala, S. (2016). Development of Lentiviral Vectors for Targeted Integration and Protein Delivery. In: Federico, M. (eds) Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools. Methods in Molecular Biology, vol 1448. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3753-0_14

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  • DOI: https://doi.org/10.1007/978-1-4939-3753-0_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3751-6

  • Online ISBN: 978-1-4939-3753-0

  • eBook Packages: Springer Protocols

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