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High-Density Peptide Arrays for Malaria Vaccine Development

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Vaccine Design

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1403))

Abstract

The development of an efficacious and practicable vaccine conferring sterile immunity towards a Plasmodium infection represents a not yet achieved goal. A crucial factor for the impact of a given anti-plasmodial subunit vaccine is the identification of the most potent parasitic components required to induce protection from both infection and disease. Here, we present a method based on a novel high-density peptide array technology that allows for a flexible readout of malaria antibodies. Peptide arrays applied as a screening method can be used to identify novel immunogenic antibody epitopes under a large number of potential antigens/peptides. Ultimately, discovered antigen candidates and/or epitope sequences can be translated into vaccine prototype design. The technology can be further utilized to unravel antibody-mediated immune responses (e.g., involved in the establishment of semi-immunity) and moreover to confirm vaccine potency during the process of clinical development by verifying the induced antibody responses following vaccination.

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Acknowledgements

We thank Ann-Kristin Mueller for critical discussions and helpful comments on the manuscript. This work was supported by the University Heidelberg Frontier Innovation Fund of the Excellence Cluster (0077.3.5.2.86) to K.H., the Carl-Zeiss-Foundation, the Gips-Schuele-Foundation, and the Karlsruhe House of Young Scientists to F.L. J.P. is the recipient of an HRCMM (Heidelberg Research Center for Molecular Medicine) Career Development Fellowship.

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Authors and Affiliations

  1. Karlsruhe Institute of Technology, Institute of Microstructure Technology, Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Karlsruhe, Germany

    Felix F. Loeffler

  2. Center for Infectious Diseases, Parasitology Unit, Heidelberg University Hospital, Im Neuenheimer Feld 324, 69120, Heidelberg, Germany

    Johannes Pfeil & Kirsten Heiss

  3. Center for Pediatric and Adolescent Medicine, General Pediatrics, Heidelberg University Hospital, Heidelberg, Germany

    Johannes Pfeil

  4. Heidelberg, Germany

    Kirsten Heiss

  5. 4 MalVa GmbH, Heidelberg, Germany

    Kirsten Heiss

Authors
  1. Felix F. Loeffler
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  2. Johannes Pfeil
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  3. Kirsten Heiss
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Corresponding authors

Correspondence to Felix F. Loeffler or Kirsten Heiss .

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Editors and Affiliations

  1. Lankenau Institute for Medical Research, Wynnewood, Pennsylvania, USA

    Sunil Thomas

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Loeffler, F.F., Pfeil, J., Heiss, K. (2016). High-Density Peptide Arrays for Malaria Vaccine Development. In: Thomas, S. (eds) Vaccine Design. Methods in Molecular Biology, vol 1403. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3387-7_32

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  • DOI: https://doi.org/10.1007/978-1-4939-3387-7_32

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3385-3

  • Online ISBN: 978-1-4939-3387-7

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