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Studying Glycosaminoglycan–Protein Interactions Using Capillary Electrophoresis

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Glycosaminoglycans

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1229))

Abstract

Methods for studying interactions between glycosaminoglycans (GAGs) and proteins have assumed considerable significance as their biological importance increases. Capillary electrophoresis (CE) is a powerful method to study these interactions due to its speed, high efficiency, and low sample/reagent consumption. In addition, CE works effectively under a wide range of physiologically relevant conditions. This chapter presents state-of-the-art on CE methods for studying GAG–protein interactions including affinity capillary electrophoresis (ACE), capillary zone electrophoresis (CZE), frontal analysis (FA)/frontal analysis continuous capillary electrophoresis (FACCE), and capillary electrokinetic chromatography (CEC) with detailed experimental protocols for ACE and CZE methods.

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Acknowledgments

This work was supported by grants SC EPSCoR/IDeA and SCICU to AL and grants HL090586 and HL107152 from the National Institutes of Health to URD. We thank Ms. Yingzi Jin of VCU for helping with the preparation of this chapter.

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Authors and Affiliations

  1. Department of Physical Sciences, Charleston Southern University, 9200 University Boulevard, North Charleston, SC, 29406, USA

    Aiye Liang

  2. Department of Medicinal Chemistry and Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond, VA, 23219, USA

    Umesh R. Desai

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  1. Aiye Liang
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  2. Umesh R. Desai
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Correspondence to Aiye Liang .

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Editors and Affiliations

  1. Department of Medicinal Chemistry, University of Utah, Salt Lake City, Utah, USA

    Kuberan Balagurunathan

  2. Department of Genetics, Cell Biology and Development, The University of Minnesota, Minneapolis, Minnesota, USA

    Hiroshi Nakato

  3. Department of Medicinal Chemistry, Virginia Commonwealth University, Richmond, Virginia, USA

    Umesh R. Desai

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Liang, A., Desai, U.R. (2015). Studying Glycosaminoglycan–Protein Interactions Using Capillary Electrophoresis. In: Balagurunathan, K., Nakato, H., Desai, U. (eds) Glycosaminoglycans. Methods in Molecular Biology, vol 1229. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1714-3_28

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  • DOI: https://doi.org/10.1007/978-1-4939-1714-3_28

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1713-6

  • Online ISBN: 978-1-4939-1714-3

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