Abstract
Selenium (Se) is a rare metal found mainly in volcanic sediments. As Japan has many sulfide deposits, selenium is naturally widely distributed at low concentrations. Selenium exists in soil as soluble seleno-oxyanions, such as selenate [Se (VI)] and selenite [Se (IV)], which are highly toxic. The Japanese government has standards for soluble selenium concentrations in soil that can be achieved by water shielding or the addition of insolubilization agents. However, as these treatments leave selenium in the soil, it cannot be reused because of a risk of selenium re-elution. To solve this problem, we attempted to rapidly insolubilize soluble seleno-oxyanions in the soil by adding a bacterium, Stutzerimonas stutzeri NT-I, which reduces selenate [Se (VI)] through selenite [Se (IV)] to insoluble elemental selenium [Se (0)] and then elemental selenium to volatile dimethyl diselenide (DMDSe). We attempted purification of selenate-contaminated soil using S. stutzeri NT-I. Under optimal culture conditions, 46% of the initial selenate concentration was removed from the selenate-contaminated soil reducing Se elution below the designated standards within 72 h. These results indicate that bioremediation using S. stutzeri NT-I is effective for selenate-contaminated soil.
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References
Bullock LA, Perez M, Armstrong JG, Parnell J, Still J, Feldmann J (2018) Selenium and tellurium resources in Kisgruva Proterozoic volcanogenic massive sulphide deposit (Norway). Ore Geol Rev 99:411–424
Kilic Y, Kartal G, Timur S (2013) An investigation of copper and selenium recovery from copper anode slimes. Int J Miner Process 124:75–82
Mizutani T, Kanaya K, Osaka T (2001) Map of selenium content in soil in Japan. J Health Sci 47:407–413
Yamada H, Kamada A, Usuki M, Yanai J (2009) Total selenium content of agricultural soils in Japan. Soil Sci Plant Nutr 55:616–622
Kang Y, Yamada H, Kyuma K, Hattori T (1993) Speciation of selenium in soil. Soil Sci Plant Nutr 39:331–337
Lenz M, Janzen N, Lens PNL (2008) Selenium oxyanion inhibition of hydrogenotrophic and acetoclastic methanogenesis. Chemosphere 73:383–388
Yu R, Coffman JP, van Fleet-Stalder V, Chasteen TG (1997) Toxicity of oxyanions of selenium and of a proposed bioremediation intermediate, dimethyl selenone. Environ Toxicol Chem 16:140–145
Somogyi Z, Kiss I, Kádár I, Bakonyi G (2007) Toxicity of selenate and selenite to the potworm Enchytraeus albidus (Annelida: Enchytraeidae): a laboratory test. Ecotoxicology 16:379–384
Gworek B, Dmuchowski W, Koda E, Marecka M, Baczewska A, Brągoszewska P, Sieczka A, Osiński P (2016) Impact of the municipal solid waste Łubna landfill on environmental pollution by heavy metals. Water 8:470
Börsig N, Scheinost AC, Shaw S, Schild D, Neumann T (2018) Retention and multiphase transformation of selenium oxyanions during the formation of magnetite via iron(II) hydroxide and green rust. Dalton Trans 47:11002–11015
Kang Y, Inoue N, Rashid MM, Sakurai K (2002) Fixation of soluble selenium in contaminated soil by amorphous iron (hydr) oxide
Vasarevičius S, Danila V, Paliulis D (2019) Application of stabilized nano zero valent iron particles for immobilization of available Cd2+, Cu2+, Ni2+, and Pb2+ ions in soil. Int J Environ Res 13:465–474
Xie Y, Dong H, Zeng G, Zhang L, Cheng Y, Hou K, Jiang Z, Zhang C, Deng J (2017) The comparison of Se(IV) and Se(VI) sequestration by nanoscale zero-valent iron in aqueous solutions: the roles of solution chemistry. J Hazard Mater 338:306–312
Zambonino MC, Quizhpe EM, Jaramillo FE, Rahman A, Santiago Vispo N, Jeffryes C, Dahoumane SA (2021) Green synthesis of selenium and tellurium nanoparticles: current trends, biological properties and biomedical applications. Int J Mol Sci 22:989
Schiavon M, Pilon-Smits EAH (2017) Selenium biofortification and phytoremediation phytotechnologies: a review. J Environ Qual 46:10–19
Wadgaonkar SL, Nancharaiah YV, Esposito G, Lens PNL (2018) Environmental impact and bioremediation of seleniferous soils and sediments. Crit Rev Biotechnol 38:941–956
Tan LC, Nancharaiah YV, van Hullebusch ED, Lens PNL (2016) Selenium: environmental significance, pollution, and biological treatment technologies. Biotechnol Adv 34:886–907
Kuroda M, Notaguchi E, Sato A, Yoshioka M, Hasegawa A, Kagami T, Narita T, Yamashita M, Sei K, Soda S, Ike M (2011) Characterization of Pseudomonas stutzeri NT-I capable of removing soluble selenium from the aqueous phase under aerobic conditions. J Biosci Bioeng 112:259–264
Kagami T, Narita T, Kuroda M, Notaguchi E, Yamashita M, Sei K, Soda S, Ike M (2013) Effective selenium volatilization under aerobic conditions and recovery from the aqueous phase by Pseudomonas stutzeri NT-I. Water Res 47:1361–1368
Otsuka O, Yamashita M (2020) Selenium recovery from wastewater using the selenate-reducing bacterium Pseudomonas stutzeri NT-I. Hydrometallurgy 197:105470
Brink HG, Wessels CE, Chirwa E (2018) Pseudomonas stutzeri NT-I: optimal conditions for growth and selenate reduction. Chem Eng Trans 70:1651–1656
Job T, Hendrik B (2019) The effect of nitrogen on the reduction of selenate and selenite to elemental selenium. Chem Eng Trans 74:529–534
Ce W, Emn C (2017) Reduction of selenium by Pseudomonas stutzeri NT-I: growth, reduction and kinetics. J Bioremed Biodegrad 8
Ike M, Soda S, Kuroda M (2017) Bioprocess approaches for the removal of selenium from industrial waste and wastewater by Pseudomonas stutzeri NT-I. In: van Hullebusch ED (ed) Bioremediation of selenium contaminated wastewater. Springer, Cham, pp 57–73
Tabelin CB, Basri AHM, Igarashi T, Yoneda T (2012) Removal of arsenic, boron, and selenium from excavated rocks by consecutive washing. Water Air Soil Pollut 223:4153–4167
Tabelin CB, Sasaki R, Igarashi T, Park I, Tamoto S, Arima T, Ito M, Hiroyoshi N (2017) Simultaneous leaching of arsenite, arsenate, selenite and selenate, and their migration in tunnel-excavated sedimentary rocks: I. Column experiments under intermittent and unsaturated flow. Chemosphere 186:558–569
Tabelin CB, Igarashi T, Villacorte-Tabelin M, Park I, Opiso EM, Ito M, Hiroyoshi N (2018) Arsenic, selenium, boron, lead, cadmium, copper, and zinc in naturally contaminated rocks: a review of their sources, modes of enrichment, mechanisms of release, and mitigation strategies. Sci Total Environ 645:1522–1553
Suzuki T, Sue K, Morotomi H, Niinae M, Yokoshima M, Nakata H (2019) Immobilization of selenium(VI) in artificially contaminated kaolinite using ferrous ion salt and magnesium oxide. J Environ Chem Eng 7:102802
Manca PP, Caredda P, Orrù G (2018) The applicability of soil flushing technology in a metallurgical plant. Int J Coal Sci Technol 5:70–77
Kang Y, Nozato N, Kyuma K, Yamada H (1991) Distribution and forms of selenium in paddy soil. Soil Sci Plant Nutr 37:477–485
Funding
This study was supported in part by the JSPS KAKENHI Grant Number 16K06876. We would like to thank F. Fukuzawa for technical help.
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Otsuka, O., Yamashita, M. (2024). Purification of Selenium-Contaminated Soils Using the Selenate Reducing Bacterium Stutzerimonas stutzeri NT-I. In: Hazarika, H., Haigh, S.K., Chaudhary, B., Murai, M., Manandhar, S. (eds) Sustainable Construction Resources in Geotechnical Engineering. IC-CREST 2023. Lecture Notes in Civil Engineering, vol 448. Springer, Singapore. https://doi.org/10.1007/978-981-99-9227-0_23
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DOI: https://doi.org/10.1007/978-981-99-9227-0_23
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