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Potato Genome Editing: Recent Challenges and a Practical Procedure

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Gene Editing in Plants

Abstract

Potato (Solanum tuberosum L.) is one of the most important staple crops, accounting for the fourth highest production in the world. To date, many studies on potatoes using genome-editing technologies have been reported. These studies cover a wide range of fields including new trait development, analysis of gene functions, and improvement in genome-editing technology. Trait-related genes are one of the major targets of genome editing, leading to improvement in crop productivity and the properties of storage starch, as well as increased tolerance to abiotic and biotic stresses. Technical advances in potato genome editing facilitate precise nucleotide substitutions, the establishment of mutants of interest with high efficiency, and the elimination of CRISPR/Cas9 and selection marker genes retained in the product genomes. In this chapter, we review recent studies on genome editing in potato and our improved method that achieved high efficiency in targeted mutagenesis for the gene of interest.

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Authors and Affiliations

  1. Laboratory of Plant Gene Expression, Research Institute for Sustainable, Humanosphere, Kyoto University, Uji, Japan

    Hiroaki Kusano

  2. Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba, Japan

    Ami Takeuchi

  3. Department of Biological Science and Technology, Tokyo, University of Science, Katsushika, Tokyo, Japan

    Hiroaki Shimada

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  1. Hiroaki Kusano
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  2. Ami Takeuchi
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  3. Hiroaki Shimada
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Editor information

Editors and Affiliations

  1. Department of Life Sciences, Mody University of Science and Technology, Lakshmangarh, Rajasthan, India

    Ashwani Kumar

  2. Biotechnology, Dr. B. Lal Institute of Biotechnology, Jaipur, Rajasthan, India

    Sudipti Arora

  3. Faculty of Bioresource Sciences Department of Local Resources, Prefectural University of Hiroshima, Shobara, Japan

    Shinjiro Ogita

  4. Department of Natural Sciences, Northeastern State University, Broken Arrow, OK, USA

    Yuan-Yeu Yau

  5. Institute of Hygiene, University Hospital Münster, Münster, Germany

    Krishnendu Mukherjee

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Kusano, H., Takeuchi, A., Shimada, H. (2024). Potato Genome Editing: Recent Challenges and a Practical Procedure. In: Kumar, A., Arora, S., Ogita, S., Yau, YY., Mukherjee, K. (eds) Gene Editing in Plants. Springer, Singapore. https://doi.org/10.1007/978-981-99-8529-6_16

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