Abstract
Within plant molecular farming (PMF), the use of viruses and virus-like particles (VLPs) is increasingly gaining momentum due to the vast array of possibilities they offer. In addition to the wide application of viruses as vectors of genes for their transient expression in plants, viral particles are being exploited as natural nanoparticles amenable to production in plants and functionalization with very different purposes. One important group of plant viruses exploited in this context is formed by viruses with flexuous elongated virions of a high aspect ratio. One of these viruses is turnip mosaic virus (TuMV), a potyvirus. TuMV virions and VLPs have been produced in plants in different functionalized manners for an ample range of applications. They have also been chemically functionalized “in vitro” after purification of their natural unmodified forms. The chapter describes and discusses the work carried out so far for the development and applications of TuMV in PMF nanobiotechnology.
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Abbreviations
- CAL-B:
-
Lipase B from Candida antarctica
- CP:
-
Capsid protein
- EGCG:
-
Epigallocatechin gallate
- EGF:
-
Epithelial growth factor
- GelMA:
-
Gelatin methacryloyl
- GFP:
-
Green fluorescent protein
- LTP:
-
Lipid transfer protein
- TuMV:
-
Turnip mosaic virus
- VEGFR-3:
-
Vascular endothelial growth factor receptor 3
- VLPs:
-
Virus-like particles
- VNPs:
-
Viral nanoparticles
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Acknowledgments
Work on TuMV nanobiotechnological functionalization has benefited from different grants obtained along the years. These are P2018/BAA-4574, COV20/00114, and PanGreen-CM from the Comunidad de Madrid; RTA2015-00017-00-00 from INIA; and ARIMNet-2 618127, an ERANet project. Daniel A. Truchado is a postdoctoral associate funded by a contract of the “Margarita Salas” program of the Spanish Ministry of Universities. Sara Rincón is funded by the P2018/BAA-4574 grant.
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Truchado, D.A., Rincón, S., Zurita, L., Ponz, F. (2023). Turnip Mosaic Virus Nanoparticles: A Versatile Tool in Biotechnology. In: Kole, C., Chaurasia, A., Hefferon, K.L., Panigrahi, J. (eds) Tools & Techniques of Plant Molecular Farming. Concepts and Strategies in Plant Sciences. Springer, Singapore. https://doi.org/10.1007/978-981-99-4859-8_8
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