Abstract
Rapidly emerging countries like India and China are lifting millions of people out of poverty. This perpendicular growth in the number of people demanding access to reliable and affordable energy will drive energy demand in the decades to come. Much effort has been employed toward optimizing microbes and predominantly microalgae, to resourcefully produce compounds that can be substitute for fossil fuels. Oils acquired from algal feedstock are rich in triacylglycerols and could be converted into biodiesel via transesterification. Apart from the triacylglycerols and carbohydrates which are predominant in microalgae, there are several biomolecules like pigments and vitamins which play crucial role in pharmaceutical industries. There is an urgent need to understand which drives the production of such economic important biofuels or chemicals to improve the sustainability of the process. Integrative omics is a strong technique to know the complete system of microalgae and develop as microbial cell factories. Genomics and transcriptomics of microalgae have provided basic understanding toward lipid biosynthesis. Proteomics and metabolomics are now complementing “microalgal omics” and offer accurate functional insights into the attendant static and dynamic physiological contexts. Current chapter focuses on the application of omics approaches which considered powerful tools for a better understanding of algae cells metabolism. Then, the data would be used to develop sustainable strategies for biodiesel and by-products yield and quality improvement and a profitable microalgae industry.
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References
Altelaar AM, Munoz J, Heck AJ (2013) Next-generation proteomics: towards an integrative view of proteome dynamics. Nat Rev Genet 14:35
Amara S, Seghezzi N, Otani H, Diaz-Salazar C, Liu J, Eltis LD (2016) Characterization of key triacylglycerol biosynthesis processes in rhodococci. Sci Rep 6:1–13
Arora N, Pienkos PT, Pruthi V, Poluri KM, Guarnieri MT (2018) Leveraging algal omics to reveal potential targets for augmenting TAG accumulation. Biotechnol Adv 36(4):1274–1292
Aslam B, Basit M, Nisar MA, Khurshid M, Rasool MH (2017) Proteomics: technologies and their applications. J Chromatogr Sci 55:182–196
Awad D, Brueck T (2020) Optimization of protein isolation by proteomic qualification from Cutaneotrichosporon oleaginosus. Anal Bioanal Chem 412:449–462
Baek J, Choi J-i, Park H, Lim S, Park SJ (2016) Isolation and proteomic analysis of a Chlamydomonas reinhardtii mutant with enhanced lipid production by the gamma irradiation method. J Microbiol Biotechnol 26:2066–2075
Bai X, Song H, Lavoie M, Zhu K, Su Y, Ye H, Chen S, Fu Z, Qian H (2016) Proteomic study bring new insights into the effect of a dark stress on lipid synthesis in P tricornutum. Sci Rep 6:1–10
Barh D, Zambare V, Azevedo V (2013) Omics: applications in biomedical, agricultural, and environmental sciences. CRC Press, New York
Ben-Amotz A, Tornabene TG, Thomas WH (1985) Chemical profile of selected species of microalgae with emphasis on lipids1. J Phycol 21:72–81
Betzen C, Alhamdani MSS, Lueong S, Schröder C, Stang A, Hoheisel JD (2015) Clinical proteomics: promises, challenges and limitations of affinity arrays. Proteomics–Clin Appl 9:342–347
Bingol K (2018) Recent advances in targeted and untargeted metabolomics by NMR and MS/NMR methods. High-throughput 7:9
Bleakley S, Hayes M (2017) Algal proteins: extraction, application, and challenges concerning production. Foods 6:33
Brogan J, Li F, Li W, He Z, Huang Q, Li C-Y (2012) Imaging molecular pathways: reporter genes. Radiat Res 177:508–513
Bruderer R, Bernhardt OM, Gandhi T, Xuan Y, Sondermann J, Schmidt M, Gomez-Varela D, Reiter L (2017) Optimization of experimental parameters in data-independent mass spectrometry significantly increases depth and reproducibility of results. Mol Cell Proteomics 16(12):2296–2309
Butterfield DA, Perluigi M (2017) Redox proteomics: a key tool for new insights into protein modification with relevance to disease. Antioxid Redox Signal 26(7):277–279
Chandramouli K, Qian P-Y (2009) Proteomics: challenges, techniques and possibilities to overcome biological sample complexity. HGP 2009:239204
Chernobrovkin A, Vicente CM, Visa N, Zubarev RA (2014) Expression proteomics reveals protein targets and highlights mechanisms of action of small molecule drugs. Sct Rep 5:11176
Cooper MB, Smith AG (2015) Exploring mutualistic interactions between microalgae and bacteria in the omics age. Curr Opin Plant Biol 26:147–153
Cornett EM, Dickson BM, Krajewski K, Spellmon N, Umstead A, Vaughan RM, Shaw KM, Versluis PP, Cowles MW, Brunzelle J (2018) A functional proteomics platform to reveal the sequence determinants of lysine methyltransferase substrate selectivity. Sci Adv 4:eaav2623
Deidda M, Piras C, Bassareo PP, Dessalvi CC, Mercuro G (2015) Metabolomics, a promising approach to translational research in cardiology. IJC Metabolic Endocrine 9:31–38
Doron L, Segal Na, Shapira M (2016) Transgene expression in microalgae—from tools to applications. Front Plant Sci 7:505
Feist P, Hummon AB (2015) Proteomic challenges: sample preparation techniques for microgram-quantity protein analysis from biological samples. Int J Mol Sci 16:3537–3563
Gillham NW, Boynton JE, Hauser CR (1994) Translational regulation of gene expression in chloroplasts and mitochondria. Annu Rev Genet 28:71–93
Gu W, Li H, Zhao P, Yu R, Pan G, Gao S, Xie X, Huang A, He L, Wang G (2014) Quantitative proteomic analysis of thylakoid from two microalgae (Haematococcus pluvialis and Dunaliella salina) reveals two different high light-responsive strategies. Sci Rep 4:6661
Guarnieri MT, Pienkos PT (2015) Algal omics: unlocking bioproduct diversity in algae cell factories. Photosynth Res 123:255–263
Guarnieri MT, Nag A, Smolinski SL, Darzins A, Seibert M, Pienkos PT (2011) Examination of triacylglycerol biosynthetic pathways via de novo transcriptomic and proteomic analyses in an unsequenced microalga. PLoS One 6:e25851
Hawrot-Paw M, Koniuszy A, Gałczyńska M, Zając G, Szyszlak-Bargłowicz J (2020) Production of microalgal biomass using aquaculture wastewater as growth medium. Watermark 12:106
Hockin NL, Mock T, Mulholland F, Kopriva S, Malin G (2012) The response of diatom central carbon metabolism to nitrogen starvation is different from that of green algae and higher plants. Plant Physiol 158:299–312
Illman A, Scragg A, Shales S (2000) Increase in chlorella strains calorific values when grown in low nitrogen medium. Enzyme Microb Technol 27:631–635
Irrgang A, Weise C, Murugaiyan J, Roesler U (2015) Identification of immunodominant proteins of the microalgae Prototheca by proteomic analysis. New Microb New Infect 3:37–40
Ismaiel MM, Piercey-Normore MD, Rampitsch C (2018) Proteomic analyses of the cyanobacterium Arthrospira (spirulina) platensis under iron and salinity stress. Environ Exp Bot 147:63–74
Kageyama H, Tanaka Y, Shibata A, Waditee-Sirisattha R, Takabe T (2018) Dimethylsulfoniopropionate biosynthesis in a diatom Thalassiosira pseudonana: identification of a gene encoding MTHB-methyltransferase. Arch Biochem Biophys 645:100–106
KaiXian Q, Borowitzka MA (1993) Light and nitrogen deficiency effects on the growth and composition ofPhaeodactylum tricornutum. Appl Biochem Biotechnol 38:93–103
Kim Y, Yoo W, Lee S, Lee M (2005) Proteomic analysis of cadmium-induced protein profile alterations from marine alga Nannochloropsis oculata. Ecotoxicology 14:589–596
Lauritano C, Ferrante MI, Rogato A (2019) Marine natural products from microalgae: an-omics overview. Mar Drugs 17:269
Le Bihan T, Martin SF, Chirnside ES, van Ooijen G, Barrios-LLerena ME, O'Neill JS, Shliaha PV, Kerr LE, Millar AJ (2011) Shotgun proteomic analysis of the unicellular alga Ostreococcus tauri. J Proteomics 74:2060–2070
Lopes H, Rocha I (2017) Genome-scale modeling of yeast: chronology, applications and critical perspectives. FEMS Yeast Res 17:50
Maghembe R, Damian D, Makaranga A, Nyandoro SS, Lyantagaye SL, Kusari S, Hatti-Kaul R (2020) Omics for bioprospecting and drug discovery from bacteria and microalgae. Antibiotics 9:229
Manjasetty BA, Büssow K, Panjikar S, Turnbull AP (2012) Current methods in structural proteomics and its applications in biological sciences. 3 Biotech 2:89–113
Manzoni C, Kia DA, Vandrovcova J, Hardy J, Wood NW, Lewis PA, Ferrari R (2016) Genome, transcriptome and proteome: the rise of omics data and their integration in biomedical sciences. Brief Bioinform 19:286–302
Meisburger SP, Thomas WC, Watkins MB, Ando N (2017) X-ray scattering studies of protein structural dynamics. Chem Rev 117:7615–7672
Miller R, Wu G, Deshpande RR, Vieler A, Gärtner K, Li X, Moellering ER, Zäuner S, Cornish AJ, Liu B (2010) Changes in transcript abundance in Chlamydomonas reinhardtii following nitrogen deprivation predict diversion of metabolism. Plant Physiol 154:1737–1752
Mnatsakanyan R, Shema G, Basik M, Batist G, Borchers CH, Sickmann A, Zahedi RP (2018) Detecting post-translational modification signatures as potential biomarkers in clinical mass spectrometry. Expert Rev Proteomics 15:6
Montecinos AE, Couceiro L, Peters AF, Desrut A, Valero M, Guillemin ML (2017) Species delimitation and phylogeographic analyses in the Ectocarpus subgroup siliculosi (Ectocarpales, Phaeophyceae). J Phycol 53:17–31
Naumann B, Busch A, Allmer J, Ostendorf E, Zeller M, Kirchhoff H, Hippler M (2007) Comparative quantitative proteomics to investigate the remodeling of bioenergetic pathways under iron deficiency in Chlamydomonas reinhardtii. Proteomics 7:3964–3979
Niizawa I, Espinaco BY, Leonardi JR, Heinrich JM, Sihufe GA (2018) Enhancement of astaxanthin production from Haematococcus pluvialis under autotrophic growth conditions by a sequential stress strategy. Prep Biochem Biotechnol 48:528–534
Niu L, Zhang H, Wu Z, Wang Y, Liu H, Wu X, Wang W (2018) Modified TCA/acetone precipitation of plant proteins for proteomic analysis. PLoS One 13:e0202238
Pienkos PT, Darzins A (2009) The promise and challenges of microalgal-derived biofuels. Biofuels Bioprod Biorefin 3:431–440
Radakovits R, Jinkerson RE, Darzins A, Posewitz MC (2010) Genetic engineering of algae for enhanced biofuel production. Eukaryot Cell 9:486–501
Radakovits R, Jinkerson RE, Fuerstenberg SI, Tae H, Settlage RE, Boore JL, Posewitz MC (2012) Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropsis gaditana. Nat Commun 3:686
Rai V, Karthikaichamy A, Das D, Noronha S, Wangikar PP, Srivastava S (2016) Multi-omics frontiers in algal research: techniques and progress to explore biofuels in the postgenomics world. Omics: J Integr Biol 20:387–399
Rashidi B, Dechesne A, Rydahl MG, Jørgensen B, Trindade LM (2019) Neochloris oleoabundans cell walls have an altered composition when cultivated under different growing conditions. Algal Res 40:101482
Rastogi RP, Pandey A, Larroche C, Madamwar D (2018) Algal Green Energy–R&D and technological perspectives for biodiesel production. Renew Sustain Energy Rev 82:2946–2969
Rauniyar N (2015) Parallel reaction monitoring: a targeted experiment performed using high resolution and high mass accuracy mass spectrometry. Int J Mol Sci 16:28566–28581
Reen CS, Wayne CK, Loke SP, Manickam S, Chuan LT, Yang T (2019) Isolation of protein from Chlorella sorokiniana CY1 using liquid biphasic flotation assisted with sonication through sugaring-out effect. J Oceanol Limnol 37:898–908
Rismani-Yazdi H, Haznedaroglu BZ, Bibby K, Peccia J (2011) Transcriptome sequencing and annotation of the microalgae Dunaliella tertiolecta: pathway description and gene discovery for production of next-generation biofuels. BMC Genomics 12:148
Salama E-S, Govindwar SP, Khandare RV, Roh H-S, Jeon B-H, Li X (2019) Can omics approaches improve microalgal biofuels under abiotic stress? Trends Plant Sci 24:611–624
Sasso S, Stibor H, Mittag M, Grossman AR (2018) The natural history of model organisms: from molecular manipulation of domesticated Chlamydomonas reinhardtii to survival in nature. Elife 7:e39233
Schaum CE (2019) Enhanced biofilm formation aids adaptation to extreme warming and environmental instability in the diatom Thalassiosira pseudonana and its associated bacteria. Limnol Oceanogr 64:441–460
Schmidt M, Geßner G, Luff M, Heiland I, Wagner V, Kaminski M, Geimer S, Eitzinger N, Reißenweber T, Voytsekh O (2006) Proteomic analysis of the eyespot of Chlamydomonas reinhardtii provides novel insights into its components and tactic movements. Plant Cell 18:1908–1930
Sirikhachornkit A, Suttangkakul A, Vuttipongchaikij S, Juntawong P (2018) De novo transcriptome analysis and gene expression profiling of an oleaginous microalga Scenedesmus acutus TISTR8540 during nitrogen deprivation-induced lipid accumulation. Sci Rep 8:3668
Stoffels L, Finlan A, Mannall G, Purton S, Parker BM (2019) Downstream processing of Chlamydomonas reinhardtii TN72 for recombinant protein recovery. Front Bioeng Biotechnol 7:383
Trentacoste EM, Shrestha RP, Smith SR, Glé C, Hartmann AC, Hildebrand M, Gerwick WH (2013) Metabolic engineering of lipid catabolism increases microalgal lipid accumulation without compromising growth. Proc Natl Acad Sci 110:19748–19753
Trifonova O, Lokhov P, Archakov A (2013) Postgenomics diagnostics: metabolomics approaches to human blood profiling. Omics: J Integr Biol 17:550–559
Umen JG, Olson BJ (2012) Genomics of volvocine algae. In: Advances in botanical research. Elsevier, Amsterdam, pp 185–243
Ummalyma SB (2020) Bioremediation and biomass production of microalgae cultivation in river watercontaminated with pharmaceutical effluent. Bioresour Technol 307:123233
Van Emon JM (2016) The omics revolution in agricultural research. J Agric Food Chem 64:36–44
Vladareanu L, Iliescu M, Wang H, Yongfei F, Vladareanu V, Yu H, Smarandache F (2016) CSP and “omics” technology apllied on versatile and intelligent portable platform for modeling complex bio-medical data. In: 2016 international conference on advanced mechatronic systems (ICAMechS), pp 423–428
Waghmare AG, Salve MK, LeBlanc JG, Arya SS (2016) Concentration and characterization of microalgae proteins from Chlorella pyrenoidosa. Bioresour Bioprocess 3:16
Walsh AM, Crispie F, Claesson MJ, Cotter PD (2017) Translating omics to food microbiology. Annu Rev Food Sci Technol 8:113–134
Wang D-Z, Zhang H, Zhang Y, Zhang S-F (2014) Marine dinoflagellate proteomics: current status and future perspectives. J Proteomics 105:121–132
Xu L, Fan J, Wang Q (2019) Omics application of bio-hydrogen production through green alga Chlamydomonas reinhardtii. Front Bioeng Biotechnol 7:201
Yang S, Wang W, Wei H, Van Wychen S, Pienkos PT, Zhang M, Himmel ME (2016) Comparison of nitrogen depletion and repletion on lipid production in yeast and fungal species. Energies 9:685
Yao L, Tan KWM, Tan TW, Lee YK (2017) Exploring the transcriptome of non-model oleaginous microalga Dunaliella tertiolecta through high-throughput sequencing and high performance computing. BMC bioinformatics 18:122
Yu K-H, Snyder M (2016) Omics profiling in precision oncology. Mol Cell Proteomics 15:2525–2536
Zainul Azlan N, Yusof M, Anum Y, Alias E, Makpol S (2019) Chlorella vulgaris improves the regenerative capacity of young and senescent myoblasts and promotes muscle regeneration. Oxid Med Cell Longev 2019:3520789
Zhila NO, Kalacheva GS, Volova TG (2005) Influence of nitrogen deficiency on biochemical composition of the green alga Botryococcus. J Appl Phycol 17:309–315
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Sharma, A., Shukla, S., Singh, R.P. (2020). Microalgae: Omics Approaches for Biofuel Production and Biomedical Research. In: Kashyap, B.K., Solanki, M.K., Kamboj, D.V., Pandey, A.K. (eds) Waste to Energy: Prospects and Applications. Springer, Singapore. https://doi.org/10.1007/978-981-33-4347-4_11
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