Abstract
Protein profile alterations following exposure to cadmium were examined in marine alga Nannochloropsis oculata through proteomic analysis. Alterations of the protein expression patterns following 10 μM cadmium treatment were analyzed on 2-dimensional gels. Out of 380 protein spots detected on 2-D gel using Coomassie staining, 11 spots were changed significantly following cadmium treatment. Because of the non-availability of molecular background information on this non-sequenced algal species, cross-species protein identification through ESI-Q-TOF MS/MS was used to identify altered proteins. Two newly induced proteins were identified as malate dehydrogenase orthologue and NADH dehydrogenase orthologue. One suppressed protein was identified to be glyceraldehydes 3-phosphate dehydrogenase A. Protein spot showing a 3-fold increase was identified as mitochondrial NADH: ubiquinone oxidoreductase orthologue. However, we could not find any matches in the database from ESI-Q-TOF MS/MS for the remaining seven proteins, thus only partial peptide sequences of these proteins were found.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ahner B.A., Morel F.M.M., (1995) Phytochelatin production in marine algae: II.Induction by various metals Limnol. Oceanogr. 40: 658–65
Ahner B.A., Morel F.M.M., (1999). Phytochelatins in microalgae. In: Round F.E., Chapman D.J., (eds), Progress in Phycological Research Biopress Ltd Bristol pp. 1–31
Bradley B.P., Shrader E.A., Kimmel D.G., Meiller J.C., (2002). Protein expression signatures: an application of proteomicsMar. Environ. Res. 54: 373–7
Geret F., Serafim A., Bebianno M.J., (2003). Antioxidant enzyme activities, metallothioneins and lipid peroxidation as biomarkers in Ruditapes decussates?Ecotoxicology 12: 417–26
Geisler D.A., Johansson F.I., Svensson Å..S., Rasmusson A.G., (2004). Antimycin A treatment decreases respiratory internal rotenone-insensitive NADH oxidation capacity in potato leavesBMC Plant Biol. 4: 1–10
Giegé P., Heazlewood J.L., Roessner-Tuanli U., Millar A.H., Fernie A.R., Leaver C.J., Sweetlove L.J., (2003). Enzymes of glycolysis are functionally associated with the mitochondrion in Arabidopsis cells. The Plant Cell 15: 2140–51
Gómez I., Merchán F., Fernández E., Quesada A., (2002). NADP-malate dehydrogenase from Chlamydomonas: prediction of new structural determinants for redox regulation by homology modeling. Plant Mol. Biol. 48: 211–21
Guillard R.L., Ryther J.H., (1962). Studies of marine planktonic diatoms I. Cyclotella nana Hustedt and Detonula confervacea (Cleve) Can. J. Microbiol. 8: 229–39
Hajduch M., Rakwal R., Agrawal G.K., Yonekura M., Pretova A., (2001). High-resolution two-dimensional electrophoresis separation of proteins from metal-stressed rice leaves: Drastic reductions/fragmentation of ribuloseΓêÆ1,5-bisphosphate carboxylase/oxygenase and induction of stress-related proteins Electrophoresis 22: 2824–31
Hu Y., Wang G., Chen G.Y.J., Fu X., Yao S.Q., (2003). Proteome analysis of Saccharomyces cerevisiae under metal stress by two-dimensional differential gel electrophoresis Electrophoresis 24: 1458–70
Kim S.T., Cho K.S., Jang Y.S., Kang K.Y., (2001). Two-dimensional electrophoretic analysis of rice proteins by polyethylene glycol fractionation for protein arrays Electrophoresis 22: 2103–9
Laemmli U.K., (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227: 680–5
Lee J.A., Shin H.W., Lee M.Y., (2000). Effects of various environmental stresses on the peroxidase activities from rice seedlings Korean J. Environ. Biol. 18: 331–6
Lee M.Y., (2002). Effect of Na2SO3 on the activities of antioxidant enzymes in geranium seedlings Phytochemistry 59: 493–9
Lee, M.Y. and Shin, H.W. (2003). Cadmium-induced changes in antioxidant enzymes from marine alga Nannochloropsis oculata J. Appl. Phycol. 15: 13–9
Meiller J.C., Bradley B.P., (2002) Zinc concentration effect at the organismal, cellular and subcellular levels in the eastern oyster Mar. Environ. Res. 54: 401–4
Payne C.D., Price N.M., (1999). Effects of cadmium toxicity on growth and elemental composition of marine phytoplankton J. Phycol. 35: 293–302
Pineiro C., Barros-Velazques J., Vazquez J., Figuera A., Gallardo J.M., (2003). Proteomics as a tool for the investigation of seafood and other marine products J. Proteome Res. 2: 127–35
Pinto E., Sigaud-Kutner T.C.S., Leitao M.A.S., Okamoto O.K., Morse D., Colepicolo P., (2003). Heavy metal-induced oxidative stress in algae J. Phycol. 39: 1008–18
Pistocchi R., Mormile A.M., Guerrini F., Isani G., Boni L., (2000). Increased production of extra- and intracellular metal-ligands in phytoplankton exposed to copper and cadmium J. Appl. Phycol. 12: 469–77
Sanita D.T.L., Gabbrielli R., (1999). Response to cadmium in higher plants. Environ. Exp. Bot. 41: 105–30
Shrader E.A., Henry T.R., Greeley M.S., Bradley B.P., (2003). Proteomics in zebrafish exposed to endocrine disrupting chemicals Ecotoxicology 12: 485–8
Shen S., Sharma A., Komatsu S., (2003). Characterization of proteins responsive to gibberellin in the leaf-sheath of rice (Oryza sativa L.) seedling using proteome analysis Biol. Pharm. Bull. 26: 129–36
Shepard J.L., Olsson B., Tedengren M., Bradley B.P., (2000). Protein expression signatures identified in Mytilus edulis exposed to PCBs, copper and salinity stress Mar. Environ. Res. 50: 337–40
Shevchenko A., Wilm M., Vorm O., Mann M., (1996). Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels Anal. Chem. 68: 850–58
Stauber E.J., Fink A., Markert C., Kruse O., Johanningmeier U., Hippler M., (2003). Proteomics of Chlamydomonas reinhardtii light-harvesting proteins Eukaryot. cell 2: 978–94
Tafforeau M., Verdus M.C., Charlionet R., Cabin-Flaman A., Ripoll C., (2002). Two-dimensional electrophoresis investigation of short-term response of flax seedlings to a cold shock Electrophoresis 23: 2534–40
van Wijk K.J., (2001). Challenges and prospects of plant proteomics Plant Physiol. 126: 501–8
Wang S.B., Hu Q., Sommerfeld M., Chen F., (2003). An optimized protocol for isolation of soluble proteins from microalgae for two-dimensional gel electrophoresis analysis J. Appl. Phycol. 15: 485–96
Wang S.B., Hu Q., Sommerfeld M., Chen F., (2004). Cell wall proteomics of the green alga Haematococcus pluvialis (chlorophyceae) Proteomics 4: 692–708
Whitton B.A., (1984). Algae as monitors of heavy metals in freshwaters In: Shubert L.E., (ed.), Algae as Ecological Indicators Academic Press London pp. 257–80
Whitton B.A., Burrows I.G., Kelly M.G., (1989). Use of Cladophora glomerata to monitor heavy metals in rivers J. Appl. Phycol.1: 293–9
Woo S.H., Fukuda M., Islam N., Takaoka M., Kawasaki H., Hirano H., (2002). Efficient peptide mapping and its applications to identify embryo proteins in rice proteome analysis Electrophoresis 23: 647–54
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
KIM, Y.K., YOO, W.I., LEE, S.H. et al. Proteomic Analysis of Cadmium-Induced Protein Profile Alterations from Marine Alga Nannochloropsis oculata . Ecotoxicology 14, 589–596 (2005). https://doi.org/10.1007/s10646-005-0009-5
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10646-005-0009-5