Abstract
Regenerative medicine is an integrative strategy that involves technology and basic sciences to stimulate regeneration in damaged and wounded tissues and organs. A variety of regenerative medicine therapies, such as those for wound healing and orthopedics, have been approved by the Food and Drug Administration (FDA) and are also now commercially available. The existing therapy of transplanting entire organs and tissues to heal defects and loss is hampered by a scarcity of donors and subsequent immunological problems. However, these issues could be overcome with the introduction of regenerative medicine technologies. Biosensors serve as a program interface for those technologies, allowing for real-time monitoring of system behavior for improved efficiency. Biosensors with increased sensitivity and performance have been developed anchor advancements in controlled therapies. The advent of noninvasive technologies to assess differentiation state in stem cell-based therapies is highly coveted and could be extremely beneficial. Different biosensing techniques are available for sensing different molecules. In this chapter, we will briefly discuss about regenerative therapeutics and its recent advancement in personalized medicine. Furthermore, discussion is included on different microfabrication and nanofabrication technologies that have opened a new paradigm in the field of biosensors as a potential form of technology to speed up development in various aspects of regenerative medicine.
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References
Amato L, Heiskanen A, Caviglia C, Shah F, Zór K, Skolimowski M, Madou M, Gammelgaard L, Hansen R, Seiz EG, Ramos M, Moreno TR, MartÃnez-Serrano A, Keller SS, Emnéus J (2014) Pyrolysed 3D-carbon scaffolds induce spontaneous differentiation of human neural stem cells and facilitate real-time dopamine detection. Adv Funct Mater 24(44):7042–7052. https://doi.org/10.1002/adfm.201400812
An JH, Kim SU, Park MK, Choi JW (2015) Electrochemical detection of human mesenchymal stem cell differentiation on fabricated gold nano-dot cell chips. J Nanosci Nanotechnol 15(10):7929–7934. https://doi.org/10.1166/jnn.2015.11225
Bagnaninchi PO, Drummond N (2011) Real-time label-free monitoring of adipose-derived stem cell differentiation with electric cell-substrate impedance sensing. Proc Natl Acad Sci U S A 108(16):6462–6467. https://doi.org/10.1073/pnas.1018260108
Carmichael SP, Shin J, Vaughan JW, Chandra PK, Holcomb JB, Atala AJ (2022) Regenerative medicine therapies for prevention of abdominal adhesions: a scoping review. J Surg Res 275:252–264. https://doi.org/10.1016/J.JSS.2022.02.005
Chalklen T, Jing Q, Kar-Narayan S (2020) Biosensors based on mechanical and electrical detection techniques. Sensors 20(19):5605. https://doi.org/10.3390/S20195605
Cheng J, Amin D, Latona J, Heber-Katz E, Messersmith PB (2019) Supramolecular polymer hydrogels for drug-induced tissue regeneration. ACS Nano 13(5):5493–5501. https://doi.org/10.1021/ACSNANO.9B00281/SUPPL_FILE/NN9B00281_SI_001.PDF
Cho S, Gorjup E, Thielecke H (2009) Chip-based time-continuous monitoring of toxic effects on stem cell differentiation. Ann Anat 191(1):145–152. https://doi.org/10.1016/j.aanat.2008.08.005
Deng SJ, Bickett DM, Mitchell JL, Lambert MH, Blackburn RK, Carter HL, Neugebauer J, Pahel G, Weiner MP, Moss ML (2000) Substrate specificity of human collagenase 3 assessed using a phage-displayed peptide library. J Biol Chem 275(40):31422–31427. https://doi.org/10.1074/jbc.M004538200
Desai TA (2000). Micro-and nanoscale structures for tissue engineering constructs. In: Medical engineering & physics (vol 22). www.elsevier.com/locate/medengphy
Dutta G (2020) Electrochemical biosensors for rapid detection of malaria. Mater Sci Energy Technol 3:150–158. https://doi.org/10.1016/J.MSET.2019.10.003
Dutta G, Jallow AA, Paul D, Moschou D (2019) Label-free electrochemical detection of S. mutans exploiting commercially fabricated printed circuit board sensing electrodes. Micromachines 10(9):575. https://doi.org/10.3390/MI10090575
Dutta G, Kim S, Park S, Yang H (2014) Washing-free heterogeneous immunosensor using proximity-dependent electron mediation between an enzyme label and an electrode. Anal Chem 86(9):4589–4595. https://doi.org/10.1021/AC5006487/SUPPL_FILE/AC5006487_SI_001.PDF
Dutta G, Lillehoj PB (2017) An ultrasensitive enzyme-free electrochemical immunosensor based on redox cycling amplification using methylene blue. Analyst 142(18):3492–3499. https://doi.org/10.1039/C7AN00789B
Dutta G, Lillehoj PB (2018) Wash-free, label-free immunoassay for rapid electrochemical detection of PfHRP2 in whole blood samples. Sci Rep 8(1):1–8. https://doi.org/10.1038/s41598-018-35471-8
Dutta G, Regoutz A, Moschou D (2018) Commercially fabricated printed circuit board sensing electrodes for biomarker electrochemical detection: the importance of electrode surface characteristics in sensor performance. PRO 2:741. https://doi.org/10.3390/proceedings2130741
Dutta G, Regoutz A, Moschou D (2020) Enzyme-assisted glucose quantification for a painless Lab-on-PCB patch implementation. Biosens Bioelectron 167:112484. https://doi.org/10.1016/J.BIOS.2020.112484
Engel E, Michiardi A, Navarro M, Lacroix D, Planell JA (2008) Nanotechnology in regenerative medicine: the materials side. Trends Biotechnol 26(1):39–47. https://doi.org/10.1016/J.TIBTECH.2007.10.005
Friedenstein AJ, Chailakhjan RK, Lalykina KS (1970) THE DEVELOPMENT OF FIBROBLAST COLONIES IN MONOLAYER CULTURES OF GUINEA-PIG BONE MARROW AND SPLEEN CELLS. Cell Prolif 3(4):393–403. https://doi.org/10.1111/J.1365-2184.1970.TB00347.X
Furukawa Y, Shimada A, Kato K, Iwata H, Torimitsu K (2013) Monitoring neural stem cell differentiation using PEDOT-PSS based MEA. Biochim Biophys Acta General Subjects 1830(9):4329–4333. https://doi.org/10.1016/j.bbagen.2013.01.022
Georges PC, Janmey PA (2005) Cell type-specific response to growth on soft materials. J Appl Physiol (1985) 98(4):1547–1553. https://doi.org/10.1152/JAPPLPHYSIOL.01121.2004
Hawley RG, Ramezani A, Hawley TS (2006) Hematopoietic stem cells. Methods Enzymol 419:149–179
Jahani M, Rezazadeh D, Mohammadi P, Abdolmaleki A, Norooznezhad A, Mansouri K (2020) Regenerative medicine and angiogenesis; challenges and opportunities. Adv Pharm Bull 10(4):490–501. https://doi.org/10.34172/apb.2020.061
Janmey PA, Winer JP, Murray ME, Wen Q (2009) The hard life of soft cells. Cell Motil Cytoskeleton 66(8):597–605. https://doi.org/10.1002/CM.20382
Je HJ, Kim MG, Kwon HJ (2017) Bioluminescence assays for monitoring chondrogenic differentiation and cartilage regeneration. Sensors (Switzerland) 17(6). https://doi.org/10.3390/s17061306
Jeong HC, Choo SS, Kim KT, Hong KS, Moon SH, Cha HJ, Kim TH (2017) Conductive hybrid matrigel layer to enhance electrochemical signals of human embryonic stem cells. Sensors Actuators B Chem 242:224–230. https://doi.org/10.1016/j.snb.2016.11.045
Jiaul Haque AM, Kim J, Dutta G, Kim S, Yang H (2015) Redox cycling-amplified enzymatic Ag deposition and its application in the highly sensitive detection of creatine kinase-MB. Chem Commun 51(77):14493–14496. https://doi.org/10.1039/C5CC06117B
Jung M, El-Said WA, Choi JW (2011) Fabrication of gold nanodot arrays on a transparent substrate as a nanobioplatform for label-free visualization of living cells. Nanotechnology 22(23). https://doi.org/10.1088/0957-4484/22/23/235304
Kaur H, Sharma A (2015) Biosensors: recent advancements in tissue engineering and cancer diagnosis. Biosensors J 4(2):1–3. https://doi.org/10.4172/2090-4967.1000131
Ko KW, Park SY, Lee EH, Yoo YI, Kim DS, Kim JY, Kwon TG, Han DK (2021) Integrated bioactive scaffold with polydeoxyribonucleotide and stem-cell-derived extracellular vesicles for kidney regeneration. ACS Nano 15(4):7575–7585. https://doi.org/10.1021/ACSNANO.1C01098/SUPPL_FILE/NN1C01098_SI_001.PDF
Lee JH, Lee T, Choi JW (2016) Nano-biosensor for monitoring the neural differentiation of stem cells. Nano 6(12). https://doi.org/10.3390/NANO6120224
Lee R, Kim IS, Han N, Yun S, Park KI, Yoo KH (2014) Real-time discrimination between proliferation and neuronal and astroglial differentiation of human neural stem cells. Sci Rep 4:6319. https://doi.org/10.1038/srep06319
Lee W, Choi D, Lee Y, Kim DN, Park J, Koh WG (2008) Preparation of micropatterned hydrogel substrate via surface graft polymerization combined with photolithography for biosensor application. Sensors Actuators B Chem 129(2):841–849. https://doi.org/10.1016/j.snb.2007.09.085
Li Y-Q (2010) Master stem cell transcription factors and signaling regulation. Cell Reprogram 12(1):3–13
Lindner U, Kramer J, Rohwedel J, Schlenke P (2010) Mesenchymal stem or stromal cells: toward a better understanding of their biology? Transfus Med Hemother 37(2):75–83. https://doi.org/10.1159/000290897
Ling SH, Lam YC, Chian KS (2012) Continuous cell separation using dielectrophoresis through asymmetric and periodic microelectrode array. Anal Chem 84(15):6463–6470. https://doi.org/10.1021/AC300079Q/SUPPL_FILE/AC300079Q_SI_001.PDF
Ma J, Xue L, Zhang M, Li C, Xiang Y, Liu P, Li G (2019) An electrochemical sensor for Oct4 detection in human tissue based on target-induced steric hindrance effect on a tetrahedral DNA nanostructure. Biosens Bioelectron 127:194–199. https://doi.org/10.1016/J.BIOS.2018.12.029
Maleki M, Ghanbarvand F, Behvarz MR, Ejtemaei M, Ghadirkhomi E (2014) Comparison of mesenchymal stem cell markers in multiple human adult stem cells. Int J Stem Cells 7(2):118–126. https://doi.org/10.15283/ijsc.2014.7.2.118
Mandal M, Dutta N, Dutta G (2021) Aptamer-based biosensors and their implications in COVID-19 diagnosis. Anal Methods 13(45):5400–5417. https://doi.org/10.1039/D1AY01519B
Pelham RJ, Wang YL (1997) Cell locomotion and focal adhesions are regulated by substrate flexibility. Proc Natl Acad Sci U S A 94(25):13661–13665. https://doi.org/10.1073/PNAS.94.25.13661/ASSET/29B06AAA-EF44-464A-91B5-8ED8DEB779BC/ASSETS/GRAPHIC/PQ2571755004.JPEG
Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284(5411):143–147. https://doi.org/10.1126/SCIENCE.284.5411.143/SUPPL_FILE/983855S5_THUMB.GIF
Pluripotent Stem Cells, iPs cells | Learn Science at Scitable. (n.d.). https://www.nature.com/scitable/topicpage/turning-somatic-cells-into-pluripotent-stem-cells-14431451/. Accessed 23 Mar 2022
Prakash SB, Abshire P (2008) Tracking cancer cell proliferation on a CMOS capacitance sensor chip. Biosens Bioelectron 23(10):1449–1457. https://doi.org/10.1016/j.bios.2007.12.015
R&D Systems Tools for Cell Biology Researchâ„¢ (n.d.) NEUROSCIENCE FOCUS: NEURAL STEM CELLS Neural Stem Cells & Differentiation Markers. www.RnDSystems.com/StemCells
Rogers JK, Church GM (2016) Genetically encoded sensors enable real-time observation of metabolite production. Proc Natl Acad Sci U S A 113(9):2388–2393. https://doi.org/10.1073/pnas.1600375113
Sampogna G, Guraya SY, Forgione A (2015) Regenerative medicine: historical roots and potential strategies in modern medicine. J Microsc Ultrastruct 3(3):101–107. https://doi.org/10.1016/J.JMAU.2015.05.002
Smith M, Lindackers C, Mccarthy K, Kar-Narayan S, Smith M, Lindackers C, Mccarthy K, Kar-Narayan S (2019) Enhanced molecular alignment in poly-l-lactic acid nanotubes induced via melt-press template-wetting. Macromol Mater Eng 304(3):1800607. https://doi.org/10.1002/MAME.201800607
Sue MJ, Yeap SK, Omar AR, Tan SW (2014) Application of PCR-ELISA in molecular diagnosis. Biomed Res Int 2014:653014. https://doi.org/10.1155/2014/653014
Suhito IR, Angeline N, Choo SS, Woo HY, Paik T, Lee T, Kim TH (2018) Nanobiosensing platforms for real-time and non-invasive monitoring of stem cell pluripotency and differentiation. Sensors 18(9):2755. https://doi.org/10.3390/S18092755
Szulcek R, Bogaard HJ, van Nieuw Amerongen GP (2014) Electric cell-substrate impedance sensing for the quantification of endothelial proliferation, barrier function, and motility. J Vis Exp 85:51300. https://doi.org/10.3791/51300
Takahashi K, Yamanaka S (2006) Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126(4):663–676. https://doi.org/10.1016/J.CELL.2006.07.024
Tarimeri N, Sezgintürk MK (2020) A high sensitive, reproducible and disposable immunosensor for analysis of SOX2. Electroanalysis 32(5):1065–1074. https://doi.org/10.1002/ELAN.201900557
Wang S, Qu X, Zhao RC (2012) Clinical applications of mesenchymal stem cells. J Hematol Oncol 5:19. https://doi.org/10.1186/1756-8722-5-19
Yadid M, Feiner R, Dvir T (2019) Gold nanoparticle-integrated scaffolds for tissue engineering and regenerative medicine. Nano Lett 19(4):2198–2206. https://doi.org/10.1021/ACS.NANOLETT.9B00472/ASSET/IMAGES/ACS.NANOLETT.9B00472.SOCIAL.JPEG_V03
Yea CH, Jeong HC, Moon SH, Lee MO, Kim KJ, Choi JW, Cha HJ (2016) In situ label-free quantification of human pluripotent stem cells with electrochemical potential. Biomaterials 75:250–259. https://doi.org/10.1016/j.biomaterials.2015.10.038
Zhang YS, Aleman J, Shin SR, Kilic T, Kim D, Shaegh SAM, Massa S, Riahi R, Chae S, Hu N, Avci H, Zhang W, Silvestri A, Nezhad AS, Manbohi A, de Ferrari F, Polini A, Calzone G, Shaikh N et al (2017) Multisensor-integrated organs-on-chips platform for automated and continual in situ monitoring of organoid behaviors. Proc Natl Acad Sci U S A 114(12):E2293–E2302. https://doi.org/10.1073/pnas.1612906114
Zhao W, Ji X, Zhang F, Li L, Ma L (2012) Embryonic stem cell markers. Molecules 17(6):6196–6236. https://doi.org/10.3390/molecules17066196
Acknowledgments
Authors gratefully acknowledge the Start-Up Research Grant (SRG) funded by Science & Engineering Research Board (SERB) (SRG/2020/000712), Department of Science and Technology (DST) (Government of India, Ministry of Science and Technology, (Technology Development and Transfer, TDP/BDTD/12/2021/General), Indo-German Science & Technology Centre (IGSTC) (IGSTC/Call 2019/NOMIS/22/2020-21/164), and Institute Scheme for Innovative Research and Development (ISIRD) (IIT/SRIC/ISIRD/2019–2020/17), Indian Institute of Technology Kharagpur (IIT Kharagpur), India for the financial support.
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Mandal, M., Shukla, J., Datta, B., Dutta, G. (2023). Role of Biosensors in Regenerative Therapeutics: Past, Present, and Future Prospects. In: Chakravorty, N., Shukla, P.C. (eds) Regenerative Medicine. Springer, Singapore. https://doi.org/10.1007/978-981-19-6008-6_5
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