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Enhancing Osteochondral Tissue Regeneration of Gellan Gum by Incorporating Gallus gallus var Domesticus-Derived Demineralized Bone Particle

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Biomimicked Biomaterials

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1250))

Abstract

Treatment for the osteochondral defects (ODs) is more challenging nowadays that needs to be addressed by developing alternative bone tissue engineering materials. Gellan gum (GG) is a widely used natural polysaccharide in the field of tissue engineering (TE) and regenerative medicine due to its versatile properties. There are many reports about the successful application of GG in cartilage tissue engineering and guiding bone formation. Functional coatings and porous composite materials have been introduced in next-generation materials for treating OD, whereas osteoconductive materials, such as demineralized bone particle (DBP) or bone derivatives, are used. However, modification of porosity, biocompatibility, cell proliferation, and mechanical properties is needed. DBP can activate human mesenchymal stem cells to differentiate into osteoblast cells. In this chapter, the potential application of GG with DBP in different combinations was reviewed, and the best suitable combinations were selected and further studied in small animal models for the soft and hard tissue engineering applications; also its application in the osteochondral integration fields were briefly discussed.

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References

  1. Hunter DJ (2009) Risk stratification for knee osteoarthritis progression: a narrative review. Osteoarthr Cartil 17(11):1402–1407

    Article  CAS  Google Scholar 

  2. Li X, Ding J, Wang J et al (2015) Biomimetic biphasic scaffolds for osteochondral defect repair. Regen Biomater 2(3):221–228

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Lee WY, Wang B (2017) Cartilage repair by mesenchymal stem cells: clinical trial update and perspectives. J Orthop Translat 9:76–88

    Article  PubMed  PubMed Central  Google Scholar 

  4. Csaki C, Schneider PR, Shakibaei M (2008) Mesenchymal stem cells as a potential pool for cartilage tissue engineering. Ann Anat 190(5):395–412

    Article  CAS  PubMed  Google Scholar 

  5. Murphy L, Helmick CG (2012) The impact of osteoarthritis in the United States: a population-health perspective: a population-based review of the fourth most common cause of hospitalization in U.S. adults. Orthop Nurs 31(2):85–91

    Article  PubMed  Google Scholar 

  6. Centers for Disease Control and Prevention (2010) Prevalence of doctor-diagnosed arthritis and arthritis-attributable activity limitation. United States, 2007–2009. https://www.cdc.gov/mmwr/preview/mmwrhtml/mm5939a1.htm

  7. Laskin RS (1978) Unicompartmental tibiofemoral resurfacing arthroplasty. J Bone Joint Surg Am 60(2):182–185

    Article  CAS  PubMed  Google Scholar 

  8. Jakobsen RB, Engebretsen L, Slauterbeck JR (2005) An analysis of the quality of cartilage repair studies. J Bone Joint Surg Am 87(10):2232–2239

    PubMed  Google Scholar 

  9. Lynn AK, Brooks RA, Bonfield W et al (2004) Repair of defects in articular joints. Prospects for material-based solutions in tissue engineering. J Bone Joint Surg (Br) 86(8):1093–1099

    Article  CAS  Google Scholar 

  10. Redman SN, Oldfield SF, Archer CW (2005) Current strategies for articular cartilage repair. Eur Cell Mater 9:23–32. discussion 23-32

    Article  CAS  PubMed  Google Scholar 

  11. Sgaglione NA (2004) The future of cartilage restoration. J Knee Surg 17(4):235–243

    Article  PubMed  Google Scholar 

  12. Nooeaid P, Salih V, Beier JP et al (2012) Osteochondral tissue engineering: scaffolds, stem cells and applications. J Cell Mol Med 16(10):2247–2270

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Deng C et al (2019) Micro/nanometer-structured scaffolds for regeneration of both cartilage and subchondral bone. Adv Funct Mater 29(4):1806068

    Article  CAS  Google Scholar 

  14. Deng C et al (2018) Bioactive scaffolds for regeneration of cartilage and subchondral bone interface. Theranostics 8(7):1940–1955

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Magill P, Byrne DP, Baker JF et al (2011) Review article: Osteochondral reconstruction and grafting. J Orthop Surg (Hong Kong) 19(1):93–98

    Article  Google Scholar 

  16. Das RK, Gocheva V, Hammink R et al (2016) Stress-stiffening-mediated stem-cell commitment switch in soft responsive hydrogels. Nat Mater 15(3):318–325

    Article  CAS  PubMed  Google Scholar 

  17. Liu X, Jin X, Ma PX (2011) Nanofibrous hollow microspheres self-assembled from star-shaped polymers as injectable cell carriers for knee repair. Nat Mater 10(5):398–406

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  18. Seo SJ, Mahapatra C, Singh RK et al (2014) Strategies for osteochondral repair: focus on scaffolds. J Tissue Eng 5:2041731414541850

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  19. Brittberg M, Lindahl A, Nilsson A et al (1994) Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. N Engl J Med 331(14):889–895

    Article  CAS  PubMed  Google Scholar 

  20. da Cunha Cavalcanti FM, Doca D, Cohen M et al (2015) Updating on diagnosis and treatment of chondral lesion of the knee. Rev Bras Ortop 47(1):12–20

    Article  PubMed  PubMed Central  Google Scholar 

  21. Shimomura K et al (2010) The influence of skeletal maturity on allogenic synovial mesenchymal stem cell-based repair of cartilage in a large animal model. Biomaterials 31(31):8004–8011

    Article  CAS  PubMed  Google Scholar 

  22. Makris EA, Gomoll AH, Malizos KN et al (2015) Repair and tissue engineering techniques for articular cartilage. Nat Rev Rheumatol 11(1):21–34

    Article  CAS  PubMed  Google Scholar 

  23. Mithoefer K et al (2006) Chondral resurfacing of articular cartilage defects in the knee with the microfracture technique. Surgical technique. J Bone Joint Surg Am 88(Suppl 1, Pt 2):294–304

    Article  PubMed  Google Scholar 

  24. He A, Liu L, Luo X et al (2017) Repair of osteochondral defects with in vitro engineered cartilage based on autologous bone marrow stromal cells in a swine model. Sci Rep 7:40489

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Deng C, Chang J, Wu C (2018) Bioactive scaffolds for osteochondral regeneration. J Orthop Translat 17:15–25

    Article  PubMed  PubMed Central  Google Scholar 

  26. Hunziker EB, Quinn TM, Hauselmann HJ (2002) Quantitative structural organization of normal adult human articular cartilage. Osteoarthr Cartil 10(7):564–572

    Article  CAS  Google Scholar 

  27. Zhang Y, Wang F, Tan H et al (2012) Analysis of the mineral composition of the human calcified cartilage zone. Int J Med Sci 9(5):353–360

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Noyes FR, Stabler CL (1989) A system for grading articular cartilage lesions at arthroscopy. Am J Sports Med 17(4):505–513

    Article  CAS  PubMed  Google Scholar 

  29. Acebes C, Roman-Blas JA, Delgado-Baeza E et al (2009) Correlation between arthroscopic and histopathological grading systems of articular cartilage lesions in knee osteoarthritis. Osteoarthr Cartil 17(2):205–212

    Article  CAS  Google Scholar 

  30. Custers RJH, Creemers LB, Verbout AJ et al (2007) Reliability, reproducibility and variability of the traditional histologic/histochemical grading system vs the new OARSI osteoarthritis cartilage histopathology assessment system. Osteoarthr Cartil 15(11):1241–1248

    Article  CAS  Google Scholar 

  31. Ulrich-Vinther M, Maloney MD, Schwarz EM et al (2003) Articular cartilage biology. J Am Acad Orthop Surg 11(6):421–430

    Article  PubMed  Google Scholar 

  32. Curl WW, Krome J, Gordon ES et al (1997) Cartilage injuries: a review of 31,516 knee arthroscopies arthroscopy. Arthroscopy 13(4):456–460

    Article  CAS  PubMed  Google Scholar 

  33. Upmeier H, Brüggenjürgen B, Weiler A et al (2007) Follow-up costs up to 5 years after conventional treatments in patients with cartilage lesions of the knee. Knee Surg Sports Traumatol Arthrosc 15(3):249–257

    Article  CAS  PubMed  Google Scholar 

  34. McNickle AG, Provencher MT, Cole BJ (2008) Overview of existing cartilage repair technology. Sports Med Arthrosc Rev 16(4):196–201

    Article  PubMed  Google Scholar 

  35. Gantar A, da Silva LP, Oliveira JM et al (2014) Nanoparticulate bioactive-glass-reinforced gellan-gum hydrogels for bone-tissue engineering. Mater Sci Eng C 43:27–36

    Article  CAS  Google Scholar 

  36. Khang G et al (2015) Biological evaluation of intervertebral disc cells in different formulations of gellan gum-based hydrogels. J Tissue Eng Regen Med 9(3):265–275

    Article  CAS  PubMed  Google Scholar 

  37. Stevens LR, Gilmore KJ, Wallace GG et al (2016) Tissue engineering with gellan gum. Biomater Sci 4(9):1276–1290

    Article  CAS  PubMed  Google Scholar 

  38. Morris ER, Nishinari K, Rinaudo M (2012) Gelation of gellan – a review. Food Hydrocoll 28(2):373–411

    Article  CAS  Google Scholar 

  39. Fialho AM, Moreira LM, Granja AT et al (2008) Occurrence, production, and applications of gellan: current state and perspectives. Appl Microbiol Biotechnol 79(6):889–900

    Article  CAS  PubMed  Google Scholar 

  40. Kim HS et al (2019) Engineering retinal pigment epithelial cells regeneration for transplantation in regenerative medicine using PEG/gellan gum hydrogels. Int J Biol Macromol 130:220–228

    Article  CAS  PubMed  Google Scholar 

  41. Anandan D, Madhumathi G, Nambiraj NA et al (2019) Gum based 3D composite scaffolds for bone tissue engineering applications. Carbohydr Polym 214:62–70

    Article  CAS  PubMed  Google Scholar 

  42. Bonifacio MA et al (2018) Antibacterial effectiveness meets improved mechanical properties: Manuka honey/gellan gum composite hydrogels for cartilage repair. Carbohydr Polym 198:462–472

    Article  CAS  PubMed  Google Scholar 

  43. Bonifacio MA et al (2018) Data on Manuka honey/gellan gum composite hydrogels for cartilage repair. Data Brief 20:831–839

    Article  PubMed  PubMed Central  Google Scholar 

  44. Costa L, Silva-Correia J, Oliveira JM et al (2018) Gellan gum-based hydrogels for osteochondral repair. Adv Exp Med Biol 1058:281–304

    Article  CAS  PubMed  Google Scholar 

  45. Prajapati VD, Jani GK, Zala BS et al (2013) An insight into the emerging exopolysaccharide gellan gum as a novel polymer. Carbohydr Polym 93(2):670–678

    Article  CAS  PubMed  Google Scholar 

  46. Song JE, Lee SE, Cha SR et al (2016) Inflammatory response study of gellan gum impregnated duck’s feet derived collagen sponges. J Biomater Sci Polym Ed 27(15):1495–1506

    Article  CAS  PubMed  Google Scholar 

  47. Vieira S, Da Silva MA, Garet E et al (2019) Self-mineralizing Ca-enriched methacrylated gellan gum beads for bone tissue engineering. Acta Biomater 93:74–85

    Article  CAS  PubMed  Google Scholar 

  48. Vuornos K et al (2019) Bioactive glass ions induce efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels. Mater Sci Eng C Mater Biol Appl 99:905–918

    Article  CAS  PubMed  Google Scholar 

  49. Xu Z, Li Z, Jiang S et al (2018) Chemically modified Gellan gum hydrogels with tunable properties for use as tissue engineering scaffolds. ACS Omega 3(6):6998–7007

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  50. EFSA Panel on Food Additives and Nutrient Sources added to Food (ANS), Younes M, Aggett P et al (2018) Re-evaluation of gellan gum (E 418) as food additive. EFSA J 16(6):e05296

    Google Scholar 

  51. Mano JF et al (2007) Natural origin biodegradable systems in tissue engineering and regenerative medicine: present status and some moving trends. J R Soc Interface 4(17):999–1030

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  52. Ciardelli G et al (2005) Blends of poly-(epsilon-caprolactone) and polysaccharides in tissue engineering applications. Biomacromolecules 6(4):1961–1976

    Article  CAS  PubMed  Google Scholar 

  53. Oliveira JT et al (2010) Gellan gum: a new biomaterial for cartilage tissue engineering applications. J Biomed Mater Res A 93(3):852–863

    CAS  PubMed  Google Scholar 

  54. Vilela CA et al (2018) In vitro and in vivo performance of methacrylated gellan gum hydrogel formulations for cartilage repair. J Biomed Mater Res A 106(7):1987–1996

    Article  CAS  PubMed  Google Scholar 

  55. Pereira DR, Silva-Correia J, Oliveira JM et al (2018) Nanocellulose reinforced gellan-gum hydrogels as potential biological substitutes for annulus fibrosus tissue regeneration. Nanomed Nanotechnol Biol Med 14(3):897–908

    Article  CAS  Google Scholar 

  56. Baek JS et al (2019) Evaluation of cartilage regeneration in gellan gum/agar blended hydrogel with improved injectability. Macromol Res 27(6):558–564

    Article  CAS  Google Scholar 

  57. Jeon HY, Shin EY, Choi JH et al (2018) Evaluation of Saponin loaded gellan gum hydrogel scaffold for cartilage regeneration. Macromol Res 26(8):724–729

    Article  CAS  Google Scholar 

  58. Oliveira JT et al (2010) Gellan gum injectable hydrogels for cartilage tissue engineering applications: in vitro studies and preliminary in vivo evaluation. Tissue Eng Part A 16(1):343–353

    Article  CAS  PubMed  Google Scholar 

  59. Shin EY, Park JH, Shin ME et al (2019) Evaluation of chondrogenic differentiation ability of bone marrow mesenchymal stem cells in silk fibroin/gellan gum hydrogels using miR-30. Macromol Res 27(4):369–376

    Article  CAS  Google Scholar 

  60. Carvalho CR et al (2018) Gellan gum-based luminal fillers for peripheral nerve regeneration: an in vivo study in the rat sciatic nerve repair model. Biomater Sci 6(5):1059–1075

    Article  CAS  PubMed  Google Scholar 

  61. Silva-Correia J, Miranda-Gonçalves V, Salgado AJ et al (2012) Angiogenic potential of gellan-gum-based hydrogels for application in nucleus Pulposus regeneration: in vivo study. Tissue Eng Part A 18(11–12):1203–1212

    Article  CAS  PubMed  Google Scholar 

  62. Sun J, Zhou Z (2018) A novel ocular delivery of brinzolamide based on gellan gum: in vitro and in vivo evaluation. Drug Des Devel Ther 12:383–389

    Article  PubMed  PubMed Central  Google Scholar 

  63. McWilliams DF, Walsh DA, Wilson D et al (2010) Angiogenesis and nerve growth factor at the osteochondral junction in rheumatoid arthritis and osteoarthritis. Rheumatology (Oxford) 49(10):1852–1861

    Article  CAS  Google Scholar 

  64. Miyoshi E, Takaya T, Nishinari K (1996) Rheological and thermal studies of gel-sol transition in gellan gum aqueous solutions. Carbohydr Polym 30(2–3):109–119

    Article  CAS  Google Scholar 

  65. Jen AC, Wake MC, Mikos AG (1996) Review: hydrogels for cell immobilization. Biotechnol Bioeng 50(4):357–364

    Article  CAS  PubMed  Google Scholar 

  66. Ruoslahti E (1989) Proteoglycans in cell regulation. J Biol Chem 264(23):13369–13372

    Article  CAS  PubMed  Google Scholar 

  67. Douglas TEL et al (2017) Composites of gellan gum hydrogel enzymatically mineralized with calcium–zinc phosphate for bone regeneration with antibacterial activity. J Tissue Eng Regen Med 11(5):1610–1618

    Article  CAS  PubMed  Google Scholar 

  68. Douglas TEL et al (2014) Injectable self-gelling composites for bone tissue engineering based on gellan gum hydrogel enriched with different bioglasses. Biomed Mater 9(4):045014

    Article  PubMed  CAS  Google Scholar 

  69. Kang SC, Kim HJ, Kim MH (2013) Effects of Astragalus membranaceus with supplemental calcium on bone mineral density and bone metabolism in calcium-deficient Ovariectomized rats. Biol Trace Elem Res 151(1):68–74

    Article  CAS  PubMed  Google Scholar 

  70. Rasch LA, De van der Schueren MAE, Van Tuyl LHD et al (2017) Content validity of a short calcium intake list to estimate daily dietary calcium intake of patients with osteoporosis. Calcif Tissue Int 100(3):271–277

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  71. Adluri RS, Zhan L, Bagchi M et al (2010) Comparative effects of a novel plant-based calcium supplement with two common calcium salts on proliferation and mineralization in human osteoblast cells. Mol Cell Biochem 340(1–2):73–80

    Article  CAS  PubMed  Google Scholar 

  72. Yoo HS, Chung KH, Lee KJ et al (2017) Melanin extract from Gallus gallus domesticus promotes proliferation and differentiation of osteoblastic MG-63 cells via bone morphogenetic protein-2 signaling. Nutr Res Pract 11(3):190–197

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  73. Yoo HS, Kim GJ, Song DH et al (2017) Calcium supplement derived from Gallus gallus domesticus promotes BMP-2/RUNX2/SMAD5 and suppresses TRAP/RANK expression through MAPK signaling activation. Nutrients 9(5):504

    Article  PubMed Central  CAS  Google Scholar 

  74. Liu W et al (2013) Isolation and identification of antioxidative peptides from pilot-scale black-bone silky fowl (Gallus gallus domesticus Brisson) muscle oligopeptides. J Sci Food Agric 93(11):2782–2788

    Article  CAS  PubMed  Google Scholar 

  75. Han KS, Song JE, Kang SJ et al (2015) Effect of demineralized bone particle/poly(lactic-co-glycolic acid) scaffolds on the attachment and proliferation of mesenchymal stem cells. J Biomater Sci Polym Ed 26(2):92–110

    Article  CAS  PubMed  Google Scholar 

  76. Jo H, Hong M, Shim JB et al (2015) The role of demineralized bone particle in a PLGA scaffold designed to create a media equivalent for a tissue engineered blood vessel. Macromol Res 23(11):986–993

    Article  CAS  Google Scholar 

  77. Kim SH, Song JE, Lee D et al (2012) Demineralized bone particle impregnated poly(l-Lactide-co-Glycolide) scaffold for application in tissue-engineered intervertebral discs. J Biomater Sci Polym Ed 23(17):2153–2170

    Article  CAS  PubMed  Google Scholar 

  78. Song JE, Kim EY, Ahn WY et al (2015) The potential of DBP gels containing intervertebral disc cells for annulus fibrosus supplementation:in vivo. J Tissue Eng Regen Med 9(11):E98–E107

    Article  CAS  PubMed  Google Scholar 

  79. Khan SN, Cammisa FP Jr, Sandhu HS et al (2005) The biology of bone grafting. J Am Acad Orthop Surg 13(1):77–86

    Article  PubMed  Google Scholar 

  80. Gong Y, Wang C, Lai RC et al (2009) An improved injectable polysaccharide hydrogel: modified gellan gum for long-term cartilage regeneration in vitro. J Mater Chem 19(14):1968–1977

    Article  CAS  Google Scholar 

  81. Smith AM, Shelton RM, Perrie Y et al (2007) An initial evaluation of gellan gum as a material for tissue engineering applications. J Biomater Appl 22(3):241–254

    Article  CAS  PubMed  Google Scholar 

  82. Pereira DR et al (2018) Injectable gellan-gum/hydroxyapatite-based bilayered hydrogel composites for osteochondral tissue regeneration. Appl Mater Today 12:309–321

    Article  Google Scholar 

  83. Kim D, Thangavelu M, Song C et al (2019) Effect of different concentration of demineralized bone powder with gellan gum porous scaffold for the application of bone tissue regeneration. Int J Biol Macromol 134:749–758

    Article  CAS  PubMed  Google Scholar 

  84. Teti A (1992) Regulation of cellular functions by extracellular matrix. J Am Soc Nephrol 2(10 Suppl):S83

    Article  CAS  PubMed  Google Scholar 

  85. Masuda H, Ishihara S, Harada I et al (2014) Coating extracellular matrix proteins on a (3-aminopropyl)triethoxysilane-treated glass substrate for improved cell culture. BioTechniques 56(4):172–179

    Article  CAS  PubMed  Google Scholar 

  86. Relucenti M, Heyn R, Petruzziello L et al (2010) Detecting microcalcifications in atherosclerotic plaques by a simple trichromic staining method for epoxy embedded carotid endarterectomies. Eur J Histochem 54(3):e33–e33

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  87. Sieren JC et al (2010) An automated segmentation approach for highlighting the histological complexity of human lung cancer. Ann Biomed Eng 38(12):3581–3591

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  88. McMenamin PG, Djano J, Wealthall R et al (2002) Characterization of the macrophages associated with the tunica Vasculosa Lentis of the rat eye. Invest Ophthalmol Vis Sci 43(7):2076–2082

    PubMed  Google Scholar 

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Acknowledgment

This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health and Welfare, Republic of Korea (grant number: HI15C2996), and the International Research and Development Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (NRF-2017K1A3A7A03089427).

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Authors and Affiliations

  1. Department of BIN Convergence Technology, Department of Polymer Nano Science & Technology and Polymer BIN Research Center, Jeonbuk National University, Jeonju, South Korea

    Muthukumar Thangavelu, David Kim, Young Woon Jeong, Wonchan Lee, Jun Jae Jung, Jeong Eun Song & Gilson Khang

  2. 3B’s Research Group, I3Bs – Research Institute on Biomaterials, Biodegradables and Biomimetics, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, University of Minho, Guimarães, Portugal

    Rui L. Reis

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  1. Muthukumar Thangavelu
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  2. David Kim
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  3. Young Woon Jeong
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Correspondence to Gilson Khang .

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Editors and Affiliations

  1. Institute of Cell & Tissue Engineering and College of Medicine, Catholic University of Korea, Seoul, Korea (Republic of)

    Heung Jae Chun

  2. 3B’s Research Group, I3Bs – Research Institute on Biomaterials, Biodegradables and Biomimetics, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, University of Minho, Guimarães, Portugal

    Rui L. Reis

  3. Department of Industrial Engineering and BIOtech Research Center, University of Trento, Trento, Italy

    Antonella Motta

  4. Department of BIN Convergence Technology, Department of Polymer Nano Science & Technology and Polymer BIN Research Center, Jeonbuk National University, Jeonju, Korea (Republic of)

    Gilson Khang

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Thangavelu, M. et al. (2020). Enhancing Osteochondral Tissue Regeneration of Gellan Gum by Incorporating Gallus gallus var Domesticus-Derived Demineralized Bone Particle. In: Chun, H., Reis, R., Motta, A., Khang, G. (eds) Biomimicked Biomaterials. Advances in Experimental Medicine and Biology, vol 1250. Springer, Singapore. https://doi.org/10.1007/978-981-15-3262-7_6

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