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Effects of Astragalus membranaceus with Supplemental Calcium on Bone Mineral Density and Bone Metabolism in Calcium-Deficient Ovariectomized Rats

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Abstract

It has been reported that Astragalus membranaceus, an Asian traditional herb, has an estrogenic effect in vitro. To examine the possible role of A. membranaceus extract with supplemental calcium (Ca) on bone status in calcium-deficient (LCa) ovariectomized (OVX) rats, a total of 48 female rats were divided into six groups: (1) normal control, (2) sham operation with LCa (sham-LCa), (3) OVX with LCa (OVX-LCa), (4) A. membranaceus supplementation with OVX-LCa (OVX-MLCa), (5) Ca supplementation with OVX (OVX-Ca), and (6) A. membranaceus and Ca supplementation with OVX (OVX-MCa). A. membranaceus ethanol extract (500 mg/kg BW) and/or Ca (800 mg/kg BW) were administered orally for 8 weeks along with a Ca-deficient diet. Results revealed that Ca supplementation with or without A. membranaceus extract significantly improved bone mineral density, biomechanical strength, and ash weight of the femur and tibia in OVX rats. High Ca with A. membranaceus combination supplementation significantly increased the ash weight of the femur and tibia and decreased urinary Ca excretion compared with supplementation of Ca alone. Uterine weight was not changed by A. membranaceus administration in OVX rats. These results suggest that A. membranaceus extract combined with supplemental Ca may be more protective against the Ca loss of bone than A. membranaceus or supplementation of Ca alone in calcium-insufficient postmenopausal women.

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Acknowledgments

This work was supported by a grant from the Next-Generation BioGreen 21 Program (no. PJ009049), Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Department of Life Science, Gachon University, Seongnam, Gyeonggi, 461-701, Republic of Korea

    Se-Chan Kang

  2. Department of Food and Nutrition, Kangwon National University, Gyo-dong, Samcheok, Gangwon, 245-711, Republic of Korea

    Hee Jung Kim & Mi-Hyun Kim

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  1. Se-Chan Kang
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Correspondence to Mi-Hyun Kim.

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Kang, SC., Kim, H.J. & Kim, MH. Effects of Astragalus membranaceus with Supplemental Calcium on Bone Mineral Density and Bone Metabolism in Calcium-Deficient Ovariectomized Rats. Biol Trace Elem Res 151, 68–74 (2013). https://doi.org/10.1007/s12011-012-9527-1

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