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Progress and Challenges in Biodiesel Production from Microalgae Feedstock

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Microalgae Biotechnology for Development of Biofuel and Wastewater Treatment

Abstract

Increasing energy demand and limited fossil fuel sources have developed the interest of researchers toward biofuel, as it is regarded as the promising approach for continuous source of energy. Microalgae are considered as a desirable feedstock for biodiesel production due to its inherent capacity to synthesize large amount of oil. The key steps in microalgae biofuel synthesis are cell culture, cell recovery, lipid removal, and fatty acid methyl ester (FAME) production. The high cost of biodiesel production is the major bottleneck in the microalga biofuel technology. Among the four steps, harvesting and lipid extraction count more than 50% of the total cost of biodiesel production. Recently, nanoparticle engineering-based methods have been applied as a powerful tool in algae system to overcome the technical problems. Another problem is the mass cultivation of microalgae, which carries major importance because massive biomass is required for viable production of biodiesel. Closed cultivation system (photobioreactor) and open cultivation system (open raceway ponds) are emerged as a solution for mass cultivation of microalgae, but there is a need to understand the design and principle of cultivation system. In this chapter, a pragmatic and critical discussion is tried to put forward with the ongoing research on microalgae with future trends.

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References

  • Alam MA, Vandamme D, Chun W, et al. Bioflocculation as an innovative harvesting strategy for microalgae. Rev Environ Sci Biotechnol. 2016;15:1–11.

    Article  Google Scholar 

  • Aresta M, Dibenedetto A, Carone M, Colonna T, Fragale C. Production of biodiesel from macroalgae by supercritical CO2 extraction and thermochemical liquefaction. Environ Chem Lett. 2005;3:136–9.

    Article  CAS  Google Scholar 

  • Arzamendi G, Campoa I, Arguinarena E, Sanchez M, Montes M, Gandia LM. Synthesis of biodiesel with heterogeneous NaOH/alumina catalysts: comparison with homogeneous NaOH Chem. Eng J. 2007;134:123–30.

    CAS  Google Scholar 

  • Atadashi IM, Aroua MK, Aziz ARA, Sulaiman NMN. The effects of catalysts in biodiesel production: a review. J Ind Eng Chem. 2013;19(1):14–26.

    Article  CAS  Google Scholar 

  • Balasubramanian S, Allen JD, Kanitka A, Boldor D. Oil extraction from Scenedesmus obliquus using a continuous microwave system—design, optimization, and quality characterization. Bioresour Technol. 2011;102:3396–403.

    Article  PubMed  CAS  Google Scholar 

  • Barros AI, Gonçalves AL, Simoes M, Pires JCM. Harvesting techniques applied to microalgae: a review. Renew Sust Energ Rev. 2015;41:1489–500.

    Article  Google Scholar 

  • Beach ES, Eckelman MJ, Cui Z, Brentner L, Zimmerman JB. Preferential technological and life cycle environmental performance of chitosan flocculation for harvesting of the green algae Neochloris oleoabundans. Bioresour Technol. 2012;121:445–9.

    Article  PubMed  CAS  Google Scholar 

  • Benavente-Valdés JR, Méndez-Zavala A, Morales-Oyervides L, Chisti Y, Montañez J. Effects of shear rate, photoautotrophy and photoheterotrophy on production of biomass and pigments by Chlorella vulgaris. J Chem Tech Biotechnol. 2017;92(9):2453–9.

    Article  CAS  Google Scholar 

  • Bhatt NC, Panwar A, Bisht TS, Tamta S. Coupling of algal biofuel production with wastewater. Sci World J. 2014;2014(Article ID 210504):10.

    Google Scholar 

  • Borlido L, Azevedo AM, Roque ACA, Aires-Barros MR. Magnetic separation in biotechnology. Biotechnol Adv. 2013;31:1374–85.

    Article  PubMed  CAS  Google Scholar 

  • Cao H, Zhang Z, Wu X, Miao X. Direct biodiesel production from wet microalgae biomass of Chlorella pyrenoidosa through in situ transesterification. Biomed Res Int. 2013;2013:1–6.

    Google Scholar 

  • Chen CY, Yeh KL, Aisyah R, Lee DJ, Chang JS. Cultivation, photobioreactor design and harvesting of microalgae for biodiesel production: a critical review. Bioresour Technol. 2011;102:71–81.

    Article  CAS  PubMed  Google Scholar 

  • Chen G, Zhao L, Qi Y, Cui YL. Chitosan and its derivatives applied in harvesting microalgae for biodiesel production: an outlook. J Nanomater. 2014;2014:1–9. https://doi.org/10.1155/2014/217537.

    Article  CAS  Google Scholar 

  • Chew TL, Bhatia S. Catalytic processes towards the production of biofuels in a palm oil and oil palm biomass-based biorefinery. Bioresour Technol. 2008;99(17):7911–22.

    Article  PubMed  CAS  Google Scholar 

  • Chisti, Y. (2007) Biodiesel from microalgae. Biotechnol. Adv. 25 (3), 294–306. https://doi.org/10.1016/j.biotechadv.2007.02.001.

    Article  PubMed  CAS  Google Scholar 

  • Chojnacka K, Marquez-Rocha FJ. Kinetic and stoichiometric relationships of the energy and carbon metabolism in the culture of microalgae. Biotechnology. 2004;3:21–34.

    Article  Google Scholar 

  • Christenson L, Sims R. Production and harvesting of microalgae for waste water treatment, biofuels, and bioproducts. Biotechnol Adv. 2011;29:686–702.

    Article  CAS  PubMed  Google Scholar 

  • Cooney M, Young G, Nagle N. Extraction of bio-oils from microalgae. Sep Purif Rev. 2009;38:219–325.

    Article  CAS  Google Scholar 

  • Danquah MK, Ang L, Uduman N, Moheimani N, Forde GM. Dewatering of microalgal culture for biodiesel production: exploring polymer flocculation and tangential flow filtration. J Chem Technol Biotechnol. 2009;84:1078–83.

    Article  CAS  Google Scholar 

  • Deng X-Y, Gao K, Addy M, Li D, Zhang R-C, Lu Q, Ma Y-W, Cheng Y-L, Chen P, Liu Y-H, Ruan R. Cultivation of Chlorella vulgaris on anaerobically digested swine manure with daily recycling of the post-harvest culture broth. Bioresour Technol. 2018;247:716–23.

    Article  PubMed  CAS  Google Scholar 

  • De-morais MG, Costa JA. Carbondioxide fixatation by Chlorella Kessleri, Chlorella Vulgaris, Scenedesmus obliquus and Spirulina sp. cultivated in flask and vertical tubular photobioreactors. Biotechnol Lett. 2007;29(9):1349–52.

    Article  PubMed  CAS  Google Scholar 

  • Farid MS, Shariati A, Badakhshan A, Anvaripour B. Using nano-chitosan for harvesting microalga Nannochloropsis sp. Bioresour Technol. 2013;131:555–9.

    Article  PubMed  CAS  Google Scholar 

  • Farooq W, Lee HUK, Huh YS, Lee YC. Chlorella vulgaris cultivation with an additive of magnesium-aminoclay. Algal Res. 2016;17:211–6.

    Article  Google Scholar 

  • Folch MLJ, Stanley GHS. A simple method of isolation and purification of total lipids from animal tissues. J Biol Chem. 1956;226:497–509.

    Google Scholar 

  • Garzon-Sanabria AJ, Davis RT, Nikolov ZL. Harvesting Nannochloris oculata by inorganic electrolyte flocculation: effect of initial cell density, ionic strength, coagulant dosage, and media pH. Bioresour Technol. 2012;118:418–24.

    Article  PubMed  CAS  Google Scholar 

  • Georgiana DR, Mayfield SP. Exploiting diversity and synthetic biology for the production of algal biofuels. Nature. 2012;488(7411):329–35.

    Article  CAS  Google Scholar 

  • Gupta SK, Ansari FA, Bauddh K, Singh B, Nema AK, Pant KK. Harvesting of microalgae for biofuels: comprehensive performance evaluation of natural, inorganic and synthetic flocculants. Green Technol Environ Sustain. 2017:131–56.

    Google Scholar 

  • Halim R, Gladman B, Danquah MK, Webley PA. Oil extraction from microalgae for biodiesel production. Bioresour Technol. 2011;102:178–85.

    Article  CAS  PubMed  Google Scholar 

  • Halim R, Danquah MK, Webley PA. Extraction of oil from microalgae for biodiesel production: a review. Biotechnol Adv. 2012;30:709–32.

    Article  PubMed  CAS  Google Scholar 

  • Harun R, Singh M, Forde GM, Danquah MK. Bioprocess engineering of microalgae to produce a variety of consumer products. Renew Sustain Energy Rev. 2010;14:1037–47.

    Article  CAS  Google Scholar 

  • Heasman M, Diemar J, O’connor W, Sushames T, Foulkes L. Development of extended shelf life microalgae concentrate diets harvested by centrifugation for bivalve mollusks- a summary. Aquac Res. 2000;31:637–5.

    Article  Google Scholar 

  • Hitttab MA, Ghaly A, Hammouda A. Microalgae harvesting methods for industrial production of biodiesel: critical review and comparative analysis. J Fundam Renewable Energy Appl. 2015;5(2):3–26.

    Google Scholar 

  • Hu Y-R, Wang F, Wang S-K, Liu C-Z, Guo C. Efficient harvesting of marine microalgae Nannochloropsis` maritime using magnetic nanoparticles. Bioresour Technol. 2013;138:387–90.

    Article  PubMed  CAS  Google Scholar 

  • Huang GH, Chen F, Wei D, Zhang XW, Chen G. Biodiesel production by microalgal biotechnology. Appl Energy. 2010;87:38–46.

    Article  CAS  Google Scholar 

  • Iqbal J, Theegala C. Microwave assisted lipid extraction from microalgae using biodiesel as co-solvent. Algal Res. 2013;2:34–42.

    Article  Google Scholar 

  • Jacobson K, Gopinath R, Meher LC, Dalai AK. Solid acid catalyzed biodiesel production from waste cooking oil. Appl Catal B. 2008;85:86–91.

    Article  CAS  Google Scholar 

  • Kanaga K, Pandey A, Kumar S, Geetanjali. Multi-objective optimization of media nutrients for enhanced production of algae biomass and fatty acid biosynthesis from Chlorella pyrenoidosa NCIM 2738. Bioresour. Technol. 2015;200:940–50. https://doi.org/10.1016/j.biortech.2015.11.017.

    Article  PubMed  CAS  Google Scholar 

  • Kanda H, Li P, Ikehara T, Yasumoto-Hirose M. Lipids extracted from several species of natural blue–green microalgae by dimethyl ether: extraction yield and properties. Fuel. 2012;95:88–92.

    Article  CAS  Google Scholar 

  • Karpagam R, Raj KJ, Ashokkumar B, Varalakshmi P. Characterization and fatty acid profiling in two fresh water microalgae for biodiesel production: lipid enhancement methods and media optimization using response surface methodology. Bioresour Technol. 2015;188(Supplement C):177–84.

    Article  PubMed  CAS  Google Scholar 

  • Khoo HH, Sharratt PN, Das P, Balasubramanian RK, Naraharisetti PK, Shaik S. Life cycle energy and CO2 analysis of microalgae-to-biodiesel: preliminary results and comparisons. Bioresour Technol. 2011;102:5800–7.

    Article  PubMed  CAS  Google Scholar 

  • Kim B, Praveenkumar R, Lee J, Nam B, Kim DM, Lee K, Lee YC, Oh YK. Magnesium aminoclay enhances lipid production of mixotrophic Chlorella sp. KR-1 while reducing bacterial populations. Bioresour Technol. 2016;219:608–13.

    Article  PubMed  CAS  Google Scholar 

  • Kim Z-H, Park Y-S, Ryu Y-J, Lee C-G. Enhancing biomass and fatty acid productivity of Tetraselmis sp. in bubble column photobioreactors by modifying light quality using light filters. Biotechnol Bioprocess Eng. 2017;22(4):397–404.

    Article  CAS  Google Scholar 

  • Kouzu M, Kasuno T, Tajika M, Sugimoto Y, Yamanaka S, Hidaka J. Calcium oxide as a solid base catalyst for transesterification of soybean oil and its application to biodiesel production. Fuel. 2008;87(12):2798–806.

    Article  CAS  Google Scholar 

  • Kouzu M, Yamanaka S, Hidaka J, Tsunomori M. Heterogeneous catalysis of calcium oxide used for transesterification of soybean oil with refluxing methanol. Appl Catal A Gen. 2009;355:1–2.

    Article  CAS  Google Scholar 

  • Kulkarni MG, Dalai AK. Waste cooking oil — an economical source for biodiesel: a review. Ind Eng Chem Res. 2006;45:2901–13.

    Article  CAS  Google Scholar 

  • Lam MK, Lee KT, Mohamed AR. Homogeneous, heterogeneous and enzymatic catalysis for transesterification of high free fatty acid oil (waste cooking oil) to biodiesel: a review. Biotechnol Adv. 2010;28(4):500–18.

    Article  PubMed  CAS  Google Scholar 

  • Lardon L, Hélias A, Sialve B, Steyer JP, Bernard O. Life-cycle assessment of biodiesel production from microalgae. Environ Sci Technol. 2009;43:6475–81.

    Article  PubMed  CAS  Google Scholar 

  • Lee Y-C, Kim B, Farooq W, Chung J, Hang J-I, Shin H-J, Jeong SH, Park J-Y, Lee J-S, Oh Y-K. Harvesting of oleaginous Chlorella sp. by organoclays. Bioresour Technol. 2013;132:440–5.

    Article  PubMed  CAS  Google Scholar 

  • Lee Y-C, Lee K, Hwang Y, Andersen HR, Kim B, Lee SY, Choi M-H, Park J-Y, Han Y-K, Oh YK, Huh Y-S. Aminoclay-templated nanoscale zerovalent iron (nZVI) synthesis for efficient harvesting of oleaginous microalga Chlorella sp. KR-1. RSC Adv. 2014;4:4122–7.

    Article  CAS  Google Scholar 

  • Lee Y-C, Lee HU, Lee K, Kim B, Lee SY, Choi M-H, Farooq W, Choi JS, Park J-Y, Lee J, Oh Y-K, Huh YS. Aminoclay-conjugated TiO2 synthesis for simultaneous harvesting and wet-disruption of oleaginous Chlorella sp. Chem Eng J. 2014a;245:143–9.

    Article  CAS  Google Scholar 

  • Lee K, Lee SY, Praveenkumar R, Kim B, Seo JY, Jeon SG, Na J-G, Park J-Y, Kim D-M, Oh Y-K. Repeated use of stable magnetic flocculant for efficient harvest of oleaginous Chlorella sp. Bioresour Technol. 2014b;167:284–90.

    Article  PubMed  CAS  Google Scholar 

  • Lee Y-C, Lee K, Oh Y-K. Recent nanoparticle engineering advances in microalgal cultivation and harvesting processes of biodiesel production: a review. Bioresour Technol. 2015;184:63–72.

    Article  PubMed  CAS  Google Scholar 

  • Liang YN, Sarkany N, Cui Y. Biomass and lipid productivities of Chlorella vulgaris under autotrophic, heterotrophic and mixotrophic growth conditions. Biotechnol Lett. 2009;31:1043–9.

    Article  CAS  PubMed  Google Scholar 

  • Lim JK, Chieh DCJ, Jalak SA, Toh PY, Yasin NHM, Ng BW, Ahmad AL. Rapid magnetophoretic separation of microalgae. Small. 2012;8:1683–92.

    Article  PubMed  CAS  Google Scholar 

  • Lotero E, Liu Y, Lopez DE, Suwannakarn K, Bruce DA, Goodwin JG Jr. Synthesis of biodiesel via acid catalysis. Ind Eng Chem Res. 2005;44:5353–63.

    Article  CAS  Google Scholar 

  • Mandal S, Mallick N. Microalga Scenedesmus obliquus as a potential source for biodiesel production. Appl Microbiol Biotechnol. 2009;84:281–91.

    Article  PubMed  CAS  Google Scholar 

  • Mao X, Wu T, Sun D, Zhang Z, Chen F. Differential responses of the green microalga Chlorella zofingiensis to the starvation of various nutrients for oil and astaxanthin production. Bioresour Technol. 2018;249:791–8.

    Article  PubMed  CAS  Google Scholar 

  • Mata TM, Martins AA, Caetano NS. Microalgae for biodiesel production and other applications: a review. Renew Sustain Energy Rev. 2010;14(1):217–32.

    Article  CAS  Google Scholar 

  • Milledge JJ, Heaven S. A review of the harvesting of micro-algae for biofuel production. Rev Environ Sci Biotechnol. 2013;12(2):165–78.

    Article  Google Scholar 

  • Molina Grima E, Belarbi E-H, Acien-Fernandez FG, Robles Medina A, Yusuf C. Recovery of microalgal biomass and metabolites: process options and economics. Biotechnol Adv. 2003;20:491–515.

    Article  CAS  PubMed  Google Scholar 

  • Mubarak M, Shaija A, Suchithra TV. A review on the extraction of lipid from microalgae for biodiesel production. Algal Res. 2015;7:117–23.

    Article  Google Scholar 

  • Munoz R, Guieysse B. Algal-bacterial processes for the treatment of hazardous contaminants: a review. Water Res. 2006;40:2799–815.

    Article  PubMed  CAS  Google Scholar 

  • Narasimharao K, Lee A, Wilson K. Catalysts in production of biodiesel: a review. J Biobased Mater Bioenergy. 2007;1(3):1–12.

    Google Scholar 

  • Neto AMP, Sotana de Souza RA, Leon-Nino AD, da Costa JDA, Tiburcio RS, Nunes TA, Sellare de Mello TC, Kanemoto FT, Saldanha-Corrêa FMP, Gianesella SMF. Improvement in microalgae lipid extraction using a sonication-assisted method. Renew Energy. 2013;55:525.

    Article  CAS  Google Scholar 

  • Ogbonna JC, Tanaka H. Cyclic autotrophic/heterotrophic cultivation of photosynthetic cells: a method of achieving continuous cell growth under light dark cycles. Bioresour Technol. 1998;65:65–72.

    Article  CAS  Google Scholar 

  • Oyler JR. Integrated processes and systems for production of biofuels using algae. Patent No. US20110136217 A1; 2009.

    Google Scholar 

  • Polishchuk A, Valev D, Tarvainen M, Mishra S, Kinnunen V, Antal T, Yang B, Rintala J, Tyystjärvi E. Cultivation of Nannochloropsis for eicosapentaenoic acid production in wastewaters of pulp and paper industry. Bioresour Technol. 2015;193:469–76.

    Article  PubMed  CAS  Google Scholar 

  • Pragya N, Pandey KK, Sahoo PK. A review on harvesting, oil extraction and biofuels production technologies from microalgae. Renew Sustain Energy Rev. 2013;24:159–71.

    Article  CAS  Google Scholar 

  • Prochazkova G, Safarik I, Branyik T. Harvesting microalgae with microwave synthesized magnetic microparticles. Bioresour Technol. 2013;130:472–7.

    Article  PubMed  CAS  Google Scholar 

  • Rai MP, Nigam S, Shrama R. Response of growth and fatty acid compositions of Chlorella pyrenoidosa under mixotrophic cultivation with acetate and glycerol for bioenergy application. Biomass Bioenergy. 2013;58:251–7.

    Article  CAS  Google Scholar 

  • Rawat I, Ranjith Kumar R, Mutanda T, Bux F. Dual role of microalgae: phycoremediation of domestic waste water and biomass production for sustainable biofuels production. Appl Energy. 2011;88:3411–24.

    Article  CAS  Google Scholar 

  • Rodrigues MA, da Silva Bon EP. Evaluation of Chlorella (Chlorophyta) as source of fermentable sugars via cell wall enzymatic hydrolysis. Enzyme Res. 2011; https://doi.org/10.4061/2011/405603.

    Article  CAS  Google Scholar 

  • Sagiroglu A, Selen I, Ozcan M, Paluzar H, Toprakkiran N. Comparison of biodiesel productivities of different vegetable oils by acidic catalysis. Chem Ind Chem Eng Q. 2011;17:53–8.

    Article  CAS  Google Scholar 

  • San Pedro A, González-López CV, Acién FG, Molina-Grima E. Marine microalgae selection and culture conditions optimization for biodiesel production. Bioresour Technol. 2013;134:353–61.

    Article  PubMed  CAS  Google Scholar 

  • Sathish A, Sims RC. Biodiesel from mixed culture algae via a wet lipid extraction procedure. Bioresour Technol. 2012;118:643–7.

    Article  CAS  PubMed  Google Scholar 

  • Shelef G, Sukenik A, Green M. Microalgae harvesting and processing: a literature review. Haifa: Technion Research and Development Foundation Ltd; 1984.

    Book  Google Scholar 

  • Shin YS, Choi H, Choi JW, Lee JS, Sung YJ, Jun S. Multilateral approach on enhancing economic viability of lipid production from microalgae: a review. Bioresour Technol. 2018;258:335–44.

    Article  PubMed  CAS  Google Scholar 

  • Show K-Y, Lee D-J. Algal biomass harvesting. In: Pandey A, Lee D-J, Chisti Y, Soccol CR, editors. Biofuels from algae. Burlington: Elsevier; 2014. p. 85–110.

    Chapter  Google Scholar 

  • Suali E, Sarbatly R. Conversion of microalgae to biofuel. Renew Sustain Energy Rev. 2012;16:4316–42.

    Article  CAS  Google Scholar 

  • Surendhiran D, Vijay M. Effect of various pretreatment for extracting intracellular lipid from Nannochloropsis oculata under nitrogen replete and depleted conditions. ISRN Chem Eng. 2014;2014:1–9. https://doi.org/10.1155/2014/536310.

    Article  CAS  Google Scholar 

  • Toh PY, Ng BW, Ahmad AL, Chan DJC, Lim JK. The role of particle-to cell interactions in dictating nanoparticle aided magnetophoretic separation of microalgal cell. Nanoscale. 2014;6(21):12838–48. https://doi.org/10.1039/C4NR03121K.

    Article  PubMed  CAS  Google Scholar 

  • Topare NS, Raut SJ, Renge VC, Khedkar SV, Chavan YP, Bhagat SL. Extraction of oil from algae by solvent extraction and oil expeller method. Int J Chem Sci. 2011;9:1746–50.

    CAS  Google Scholar 

  • Uduman N, Qi Y, Danquah MK, Forde GM, Hoadley A. Dewatering of microalgal cultures: a major bottle neck to algae-based fuels. J Renew Sustain Energy. 2010;2:012701–15.

    Article  CAS  Google Scholar 

  • Wang G, Wang T. Characterization of lipid components in two microalgae for biofuel application. J Am Oil Chem Soc. 2011;89:135–43.

    Article  CAS  Google Scholar 

  • Wiley PE, Campbell JE, McKuin B. Production of biodiesel and biogas from algae: a review of process train options. Water Environ Res. 2011;83(4):326–38.

    Article  PubMed  CAS  Google Scholar 

  • Xiong W, Li XF, Xiang JY, Wu QY. High-density fermentation of microalga Chlorella protothecoides in bioreactor for microbio-diesel production. Appl Microbiol Biotechnol. 2008;78:29–36.

    Article  PubMed  CAS  Google Scholar 

  • Xu H, Miao XL, Wu QY. High quality biodiesel production from a microalga Chlorella protothecoides by heterotrophic growth in fermenters. J Biotechnol. 2006;126:499–507.

    Article  PubMed  CAS  Google Scholar 

  • Xu L, Guo C, Wang F, Zheng S, Liu C-Z. A simple and rapid harvesting method for microalgae by in situ magnetic separation. Bioresour Technol. 2011;102:10047–51.

    Article  PubMed  CAS  Google Scholar 

  • Yoo G, Park WK, Kim CW, Choi YE, Yang JW. Direct lipid extraction from wet Chlamydomonas reinhardtii biomass using osmotic shock. Bioresour Technol. 2012;123:717–22.

    Article  PubMed  CAS  Google Scholar 

  • Yu S, Min Sk, Shin HW. Nanocellulose size regulates microalgal flocculation and lipid metabolism. Sci Rep. 2016;6:35684. https://doi.org/10.1038/srep35684.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Zabeti M, Wan Daud WMA, Aroua MK. Activity of solid catalysts for biodiesel production: a review. Fuel Process Technol. 2009;90:770–7.

    Article  CAS  Google Scholar 

  • Zheng H, Yin J, Gao Z, Huang H, Ji X, Dou C. Disruption of Chlorella vulgaris cells for the release of biodiesel producing lipids: a comparison of grinding, ultrasonication, bead milling, enzymatic lysis, and microwaves. Appl Biochem Biotechnol. 2011;164:1215–24.

    Article  CAS  PubMed  Google Scholar 

  • Zhou W, Min M, Hu B, Ma X, Liu Y, Wang Q, et al. Filamentous fungi assisted bio-flocculation: a novel alternative technique for harvesting heterotrophic and autotrophic microalgal cells. Sep Purif Technol. 2013;107:158–65.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India

    Shrasti Vasistha, Anwesha Khanra & Monika Prakash Rai

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  1. Shrasti Vasistha
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  2. Anwesha Khanra
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  1. Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou, China

    Md. Asraful Alam

  2. Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou, China

    Zhongming Wang

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Vasistha, S., Khanra, A., Rai, M.P. (2019). Progress and Challenges in Biodiesel Production from Microalgae Feedstock. In: Alam, M., Wang, Z. (eds) Microalgae Biotechnology for Development of Biofuel and Wastewater Treatment. Springer, Singapore. https://doi.org/10.1007/978-981-13-2264-8_14

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