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Biotechnology of Medicinal Plants in Taiwan: Studies on In Vitro Propagation and Influence of Ventilation Closures on Hyperhydricity in Cultures

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Medicinal Plants - Recent Advances in Research and Development

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Abstract

Medicinal plants have played a significant role in maintaining human health and improving the quality of life for thousands of years. In the past few decades, there has been an exponential rise in demand for herbal medicines, which in turn has resulted in an overexploitation and dwindling supply of medicinal plants in the wild. Therefore, it is imperative to explore all possible modes of plant propagation and large-scale cultivation. Taiwan is the home of many highly valued medicinal herbs used in the traditional Chinese medicines. In our laboratories, for more than two decades, tissue culture techniques have been used successfully for the propagation of several medicinally important plant species. The present article reviews the studies carried out on in vitro propagation of selected medicinal plants in Taiwan (Glossogyne tenuifolia, Saussurea involucrata, Gentiana scabra, and Drynaria fortunei). The article also includes the studies on induction of somatic embryogenesis in medicinal herb Peucedanum japonicum and influence of different ventilation closures on hyperhydricity (vitrification) in cultures of Scrophularia yoshimurae, Bupleurum kaoi, Gentiana scabra, and Glossogyne tenuifolia.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

ABA:

Abscisic acid

AF:

Aluminum foil

BA:

6-Benzyladenine

CYUT:

Chaoyang University of Technology

DP:

Dispense paper

DPPH:

2,2-Diphenyl-1-picrylhydrazyl

EC50:

Effective concentration (half maximal)

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

LED:

Light-emitting diode

MS:

Murashige and Skoog’s medium

NAA:

α-Naphthaleneacetic acid

SEM:

Scanning electron microscopy

TARI:

Taiwan Agricultural Research Institute

TEAC:

Trolox equivalent antioxidant capacity

TFTF:

Tree fern trunk fiber

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Acknowledgments

Financial support in the form of research grants (NSC-91-2317-B-324-001, NSC-91-2313-B-324-002, and NSC-94-2317-B-324-001) from the National Science Council (94 Agri Sci-5.2.1-Sci-al) and from the Council of Agriculture, Taiwan, is gratefully acknowledged.

Disclosure

The authors declare that all the experiments undertaken in the case of Saussurea involucrata comply with the current laws of Taiwan.

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Authors and Affiliations

  1. Department of Applied Chemistry, Chaoyang University of Technology, Taichung, Taiwan

    Dinesh Chandra Agrawal, Hung-Chi Chang, Emily Chin-Fun Chen & Hsin-Sheng Tsay

  2. Department of Golden-Ager Industry Management, Chaoyang University of Technology, Taichung, Taiwan

    Hung-Chi Chang

  3. Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, Taiwan

    Chia-Chen Chen & Chao-Lin Kuo

  4. Department of Agronomy, National Chung Hsing University, Taichung, Taiwan

    Hsin-Sheng Tsay

Authors
  1. Dinesh Chandra Agrawal
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  2. Hung-Chi Chang
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  3. Chia-Chen Chen
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  4. Chao-Lin Kuo
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  5. Emily Chin-Fun Chen
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  6. Hsin-Sheng Tsay
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Corresponding authors

Correspondence to Dinesh Chandra Agrawal or Hsin-Sheng Tsay .

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Editors and Affiliations

  1. Department of Applied Chemistry, Chaoyang University of Technology, Taichung, Taiwan

    Hsin-Sheng Tsay

  2. Agricultural Biotechnology Research Center, Taipei, Taiwan

    Lie-Fen Shyur

  3. Department of Applied Chemistry, Chaoyang University of Technology, Taichung, Taiwan

    Dinesh Chandra Agrawal

  4. School of Pharmacy and Chinese Herbal Medicine Research Center, China Medical University, Taichung, Taiwan

    Yang-Chang Wu

  5. College of Agriculture and Natural Resources, Department of Forestry, National Chung Hsing University, Taichung, Taiwan

    Sheng-Yang Wang

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Agrawal, D., Chang, HC., Chen, CC., Kuo, CL., Chen, E.CF., Tsay, HS. (2016). Biotechnology of Medicinal Plants in Taiwan: Studies on In Vitro Propagation and Influence of Ventilation Closures on Hyperhydricity in Cultures. In: Tsay, HS., Shyur, LF., Agrawal, D., Wu, YC., Wang, SY. (eds) Medicinal Plants - Recent Advances in Research and Development. Springer, Singapore. https://doi.org/10.1007/978-981-10-1085-9_9

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