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A sex-linked microsatellite locus isolated from the Y chromosome of lake charr, Salvelinus namaycush

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Ecology, behaviour and conservation of the charrs, genus Salvelinus

Part of the book series: Developments in environmental biology of fishes ((DEBF,volume 22))

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Synopsis

A small-insert library was constructed after microdissection of the short arm of the Y chromosome of lake charr, Salvelinus namaycush. Clones from this library were sequenced and two dinucleotide CA-repeat microsatellite sequences were recovered. Oligonucleotide primers for one locus were designed and optimized for amplification by PCR. This locus (designated Yp136) was tested for sex linkage in twelve lake charr families and found to be a distance of 37 centimorgans (cM) from the sex-determining locus. This microsatellite locus was also examined in three informative, gynogenetic/diploid, lake charr crosses for linkage to the centromere on the X chromosome. Results from these families show that this locus is 19 cM from the centromere. The combination of linkage data and microdissection information places the sex-determining locus near the telomere of the Y chromosome in lake charr. This is consistent with studies of several other fish species including some salmonids that place the sex locus near the telomere.

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Authors and Affiliations

  1. Department of Biological Sciences, University of Wisconsin-Milwaukee, PO Box 413, Milwaukee, WI, 53201, USA

    Jake Stein

  2. Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, Veterinary Science Building, 1971 Commonwealth Avenue, St. Paul, MN, 55108, USA

    Kent M. Reed

  3. Ontario Ministry of Natural Resources, Trent University, Peterborough, Ontario, K9J 8N8, Canada

    Chris C. Wilson

  4. School of Biological Sciences, Washington State University, 14204 NE Salmon Creek Ave., Vancouver, WA, 98686-9600, USA

    Ruth B. Phillips

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  1. Jake Stein
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  2. Kent M. Reed
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  3. Chris C. Wilson
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  4. Ruth B. Phillips
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Correspondence to Ruth B. Phillips .

Editor information

Pierre Magnan Céline Audet Hélène Glémet Michel Legault Marco A. Rodríguez Eric B. Taylor

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© 2002 Springer Science+Business Media Dordrecht

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Stein, J., Reed, K.M., Wilson, C.C., Phillips, R.B. (2002). A sex-linked microsatellite locus isolated from the Y chromosome of lake charr, Salvelinus namaycush . In: Magnan, P., Audet, C., Glémet, H., Legault, M., Rodríguez, M.A., Taylor, E.B. (eds) Ecology, behaviour and conservation of the charrs, genus Salvelinus . Developments in environmental biology of fishes, vol 22. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1352-8_18

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  • DOI: https://doi.org/10.1007/978-94-017-1352-8_18

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-6088-4

  • Online ISBN: 978-94-017-1352-8

  • eBook Packages: Springer Book Archive

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