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Microdissection of the Y chromosome and fluorescencein situ hybridization analysis of the sex chromosomes of lake trout,Salvelinus namaycush

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Abstract

Lake trout,Salvelinus namaycush, is one of the few salmonids with morphologically differentiated sex chromosomes. Genetic analysis suggested that the sex-determining region of this species lies on the short arm of the Y chromosome. The differential arm of the Y chromosome was microdissected and the resulting DNA amplified in a sequence-independent manner. Amplified DNA was biotin labeled as a probe for fluorescencein situ hybridization (FISH). Strong hybridization signals were seen covering defined regions of both the Y and X chromosomes. Homeologous chromosomes of the ancestrally tetraploid genome were not identified by FISH with the Y probe, indicating diploidization of this region of the genome.

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Authors and Affiliations

  1. the Department of Biological Sciences, University of Wisconsin-Milwaukee, 53201, Milwaukee, WI, USA

    K. M. Reed & R. B. Phillips

  2. the Section of Hematology/Oncology, The University of Chicago, 60637, Chicago, IL, USA

    S. K. Bohlander

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  1. K. M. Reed
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  2. S. K. Bohlander
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  3. R. B. Phillips
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Correspondence to K. M. Reed.

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Reed, K.M., Bohlander, S.K. & Phillips, R.B. Microdissection of the Y chromosome and fluorescencein situ hybridization analysis of the sex chromosomes of lake trout,Salvelinus namaycush . Chromosome Res 3, 221–226 (1995). https://doi.org/10.1007/BF00713046

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  • DOI: https://doi.org/10.1007/BF00713046

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