Abstract
Fractions rich in hemicelluloses were extracted from the depectinated residue of apples (var. Glockenapfel) at three different stages of ripeness. The fractions extracted with 4M NaOH were characterised by HPSEC, uronic acid determination and methylation analysis. Substantial amounts of galacturonic acid could be detected indicating the presence of pectic substances. An enrichment of the pectic substances was achieved by degradation of the xyloglucan with a specific endo-β-1,4-glucanohydrolase. A mixture rich in pectic polymers and xyloglucan oligomers was obtained. The pectic polymers were separated from the oligomers by ultrafiltration and characterised by methylation analysis and HPSEC. The results confirm the presence of pectic hairy regions in hemicellulose fractions of apples. The side chains of these pectic polymers consist mainly of highly ramified arabinans and linear galactans. The existence of a linkage between xyloglucans and pectins can still not be proven.
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Lutz, M.V., Oechslin, R., Amadò, R. (2003). Structural features of pectic substances from hemicellulose extracts of apples. In: Voragen, F., Schols, H., Visser, R. (eds) Advances in Pectin and Pectinase Research. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0331-4_10
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DOI: https://doi.org/10.1007/978-94-017-0331-4_10
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