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Structural features of the hemicellulose polymers of apples

Strukturkennzeichen der Hemicellulose-Polysaccharide von Äpfeln

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Zusammenfassung

Es wurde ein Hemicellulose-Extrakt aus Äpfeln durch alkalische Extraktion der entpectinisierten alkoholunlöslichen Substanz gewonnen. Dieser Extrakt wurde fraktioniert mittels Ionenaustausch-Chromatographie und Gelfiltration vor und nach Behandlung mit einer reinen 1,4-β-d-Glucanglucanohydrolase. Die Zusammensetzung der verschiedenen Fraktionen an Zuckerbausteinen und den glykosidischen Bindungen wurde bestimmt. Die Resultate deuten darauf hin, daß der Hemicellulose-Extrakt von Äpfeln Fucogalaktoxyloglucane, pektinassoziierte Arabane und Galaktane (Gluko)-Mannane, (Arabino)-Xylane und 1,3-Glucane enthält. Die Fucogalaktoxyloglucane und die Arabane und Galaktane wurden als solche und als Komplexe miteinander und mit (Arabino)-Xylanen isoliert. Der größte Teil der Mannane war nicht an andere Hemicellulosen gebunden.

Abstract

The hemicellulose extract from apples, obtained by alkaline extraction of depectinized apple alcohol-insoluble solids, was fractionated by ion-exchange chromatography on DEAE cellulose and gel filtration chromatography before and after treatment with pure 1,4-β-d-glucan glucanohydrolase. The sugar and glycosyl linkage composition of various fractions obtained were established. The results of this study indicate that the apple hemicellulose extract consists of fucogalactoxyloglucans, pectic arabinans and galactans, (gluco-)mannans, (arabino-)xylans, and 1,3 linked glucans. Fucogalaktoxyloglucans and pectic arabinans and galactans were isolated as such, and in complexes with each other and with (arabino-)xylans. The major part of the mannans is not linked to other hemicellulose polymers.

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Authors and Affiliations

  1. Department of Food Science, Agricultural University, De Dreyen 12, NL-7803, BC Wageningen, The Netherlands

    Fons G. J. Voragen, Henk A. Schols & Walter Pilnik

Authors
  1. Fons G. J. Voragen
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  2. Henk A. Schols
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  3. Walter Pilnik
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Voragen, F.G.J., Schols, H.A. & Pilnik, W. Structural features of the hemicellulose polymers of apples. Z Lebensm Unters Forch 183, 105–110 (1986). https://doi.org/10.1007/BF01041926

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  • DOI: https://doi.org/10.1007/BF01041926

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