Abstract
Experimental evaluations in plant cell culture and micropropagation very frequently include visual examinations, or arbitrary quality rankings based on obvious visible criteria. Although they are subjective and non-quantitative, visual observations do offer several advantages over numeric methods of evaluating samples. For example, changes in color of a callus or shoot, degree 146 M.A.L. Smith of hyperhydricity, density of secondary branching, incidence of root or callus initiation, and several other qualitative features can be readily detected by quick examination with the eye. Treatment differences marked by these visible criteria are recognized as important gauges of culture performance, yet would not be accounted for by routine data collection for fresh weight, shoot number, shoot length, etc. While most numeric, quantitative evaluations would require that each culture be sacrificed at the time of final evaluation, visual observations through a transparent culture vessel can be performed repeatedly without disturbing the in vitro plant’s microenvironment.
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Smith, M.A.L. (1995). Image analysis for plant cell culture and micropropagation. In: Aitken-Christie, J., Kozai, T., Smith, M.A.L. (eds) Automation and environmental control in plant tissue culture. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-8461-6_7
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DOI: https://doi.org/10.1007/978-94-015-8461-6_7
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