Skip to main content
SpringerLink
Log in

The Use of Agrobacterium for Plant Genetic Engineering

  • Chapter
The Rhizobiaceae

  • 604 Accesses

Abstract

Agrobacterium tumefaciens and A. rhizogenes are soil pathogens that induce crown gall tumors and hairy roots, respectively, on plants. During the infection a specific segment of the tumor-inducing (Ti) or root-inducing (Ri) plasmid, the T-DNA, is transferred from the bacterium to the plant cell, followed by its integration in the plant genome and subsequent expression. The T-DNA contains 1) genes encoding enzymes involved in the synthesis of phytohormones (onc genes) that cause proliferation of crown gall and hairy root cells and 2) genes which code for the production of opines, that serve as specific nutrients for the infecting bacteria. The T-DNA region on the Ti-plasmid is flanked by directly repeated 25-bp border sequences that are required in cis for its precise excision and transfer to the plant cell (Zambryski, 1992). Excision and transfer functions of the T-DNA are mediated by vir genes, located on the Ti-plasmid outside the border sequences. This DNA transfer system based on Agrobacterium T-DNA transfer has been exploited to develop host-vector systems for the introduction of gene constructs in plant species.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
eBook
USD 16.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Abel, P., Nelson, R., De, B., Hoffman, N., Rogers, S, Fraley, R. and Beachy, R. (1986) Science 232, 738–743.

    Article  PubMed  CAS  Google Scholar 

  • Allen, R. (1995) Plant Physiol. 107, 1049–1054.

    PubMed  CAS  Google Scholar 

  • Altenbach, S., Pearson, K., Meeker, G., Staraci, L. and Sun, S. (1989) Plant Molecular Biology 13, 513–522.

    Article  PubMed  CAS  Google Scholar 

  • Bechtold, N., Ellis. J. and Pelletier, G. (1993) C.R. Acad. Sci. 316, 1194–1199.

    CAS  Google Scholar 

  • Bensen, R., Johal, G., Crane, V., Tossberg, J., Schnäble, P., Meely, R. and Briggs, S. (1995) The Plant Cell, 7, 75–84.

    PubMed  CAS  Google Scholar 

  • Broglie, K., Chet, I., Holliday, M., Cressman, R., Biddle, P., Knowlton, S. Mauvais, C. and Broglie, R. (1991) Science 254, 1194–1197.

    Article  PubMed  CAS  Google Scholar 

  • Chilton, M.D. (1993) Proc. Natl. Acad. Sci. USA 90, 3119–3120.

    Article  PubMed  CAS  Google Scholar 

  • Cuozzo, M., O’Connell, K., Kaniewski, W., Fang, R., Chua, N. and Turner, N. (1988) Bio/Technol. 6, 549–557.

    Article  CAS  Google Scholar 

  • Deblaere, R., Bytebier, B., De Greve, H., Deboeck, F., Van Montagu, M., Schell, J. and Leemans, J. (1985) Nucleic Acids Res. 13, 4777–4788.

    Article  PubMed  CAS  Google Scholar 

  • De Block, M., Botterman, J., Vandewiele, M., Dockx, J., Thoens, C., GosselĂ©, V., Mowa, N., Thompson, C., Van Montagu, M. and Leemans, J. (1987) EMBO J. 6, 2513–2518.

    PubMed  CAS  Google Scholar 

  • Feldmann, K., Malmberg, R.L. and Dean, C. (1994) in Arabidopsis (Meyerowitz, E.M. and Somerville, C.R., eds), pp. 137–172, Cold Spring Harbor Laboratory Press.

    Google Scholar 

  • Fiedler, U. and Conrad, U. (1995) Bio/Technology 13, 1090–1093.

    Article  PubMed  CAS  Google Scholar 

  • Fischhoff, D., Bowdish, K., Perlak, F., Manone, P., McCormick, S., Niedermeyer, J., Fean, D., Kusano-Kretzmer, K., Mayer, E., Rochester, D., Rogers, S. and Fraley, T. (1987) Bio/Technology 5, 807–813.

    Article  CAS  Google Scholar 

  • Fritze, K., Czaja, I. and Waiden, R. (1995) Plant J. 7, 261–272.

    Article  CAS  Google Scholar 

  • Gerlach, W., Llewellyn, D. and Haseloff, J. (1987) Nature 328, 802–805.

    Article  CAS  Google Scholar 

  • Goddijn, O., Lindsey, K., van der Lee, F., Klap, J. and Sijmons, P. (1993) Plant J. 4, 863–873.

    Article  PubMed  CAS  Google Scholar 

  • Gray, J., Picton, S., Giovannoni, J. and Grierson, D. (1994) Plant Cell Environ. 17, 557–571.

    Article  CAS  Google Scholar 

  • Haldter, U., Morris, p. and Willmitzer, L. (1992) Mol. Gen. Genet. 231, 186–193.

    Google Scholar 

  • Hamilton, A., Lycett, G. and Grierson, D. (1990) Nature 346, 284–287.

    Article  CAS  Google Scholar 

  • Hamilton, C., Frary, A., Lewis, C. and Tanksley, S. (1996) Proc. Natl. Acad. Sci. USA 93, 9975–9979.

    Article  PubMed  CAS  Google Scholar 

  • Harrison, B., Mayo, M. and Baulcombe, D. (1987) Nature 328, 799–802.

    Article  Google Scholar 

  • Haughn, G., Smith, J., Mazur, B. and Somerville, C. (1988) Mol. Gen. Genet. 211, 266–271.

    Article  CAS  Google Scholar 

  • Hiei, Y., Ohta, S., Komari, T. and Kumashiro, T. (1994) Plant J. 6, 271–282.

    Article  PubMed  CAS  Google Scholar 

  • Hilder, V., Gatehouse, A., Sheerman, S., Barker, R. and Boulter, D. (1987) Nature 330, 160–163.

    Article  CAS  Google Scholar 

  • Hoekema, A., Hirsch, P., Hooykaas, P.J.J. and Schilperoort, R.A. (1983) Nature 303, 179–180.

    Article  CAS  Google Scholar 

  • Horsch, R., Fry, J., Hoffman, N., Eichholtz, D., Rogers, S. and Fraley, R. (1985) Science 227, 1229–1231.

    Article  CAS  Google Scholar 

  • Ishida, Y., Saito, H., Ohta, S., Hiei, Y., Komari, T. and Kumashiro, T. (1996) Nature Biotechnology 14, 745–750.

    Article  PubMed  CAS  Google Scholar 

  • Jones, J., Duinsmuir, P. and Bedbrook, J. (1985) EMBO J. 4, 2411–2418.

    PubMed  CAS  Google Scholar 

  • Kertbundit, S., De Greve, F., Deboeck, M., Van Montagu, M. and Hernalsteens, J.P. (1991) Proc. Natl. Acad. Sci. USA 88, 5212–5216.

    Article  PubMed  CAS  Google Scholar 

  • Komari, T. (1990) Plant Cell Rep. 9, 303–306.

    Article  CAS  Google Scholar 

  • Komari, T., Halperin, W. and Nester, E. (1986) J. Bacteriol. 166, 88–94.

    PubMed  CAS  Google Scholar 

  • Komari, T., Hiei, Y., Saito, Y., Murai, N. and Kumashiro, T. (1996) Plant J. 10, 165–174.

    Article  PubMed  CAS  Google Scholar 

  • Koncz, C., Martini, N., Mayerhofer, R., Koncz-Kalman, Z., Körber, H., Redei, G. and Schell, J. (1989) Proc. Natl. Acad. Sci. USA 86, 8467–8471.

    Article  PubMed  CAS  Google Scholar 

  • Koncz, C., NĂ©meth, K., Redei, G. and Schell, J. (1992) Plant Mol. Biol. 20, 963–976.

    Article  PubMed  CAS  Google Scholar 

  • Kridl, J., Knutzon, D., Johnson, W., Thompson, G., Radke, S., Turner, J. and Knauf, V. (1991) Molecular Biology of Plant Growth and Developent, Tucson, 723.

    Google Scholar 

  • Lee, K., Lund, P., Lowe, K. and Dunsmuir, P. (1990) Plant Cell 2, 415–425.

    PubMed  CAS  Google Scholar 

  • Lindsey, K., Wei, W., Clarke, M., McArdle, H., Rooke, L. and Topping, J. (1993) Transgenic Res. 2, 33–47.

    Article  PubMed  CAS  Google Scholar 

  • Loesch-Fries, L., Merlo, D., Zinnen, T., Burhop, L., Hill, K., Krahn, K., Jarvia, N., Nelson, S. and Halk, E. (1988) EMBO J. 6, 1845–1851.

    Google Scholar 

  • Mandai, A., Lang, V, Orczyk, W. and Palva, E.T. (1993) Theor. Appl. Genet. 86, 621–628.

    Article  Google Scholar 

  • Mariani, C., De Beuckeleer, M., Truettner, J., Leemans, J. and Goldberg, R. (1990) Nature 347, 737–741.

    Article  CAS  Google Scholar 

  • Mariani, C., GosselĂ©, V., De Beuckeleer, M., De Block, M., Goldberg, R., De Greef, W. and Leemans, J. (1992) Nature 357, 384–387.

    Article  CAS  Google Scholar 

  • Miao, Z.H. and Lam, E. (1995) Plant J. 7, 359–365.

    Article  PubMed  CAS  Google Scholar 

  • Morton, R. and Hooykaas, P. (1995) Mol. Breed. 1, 123–132.

    Article  CAS  Google Scholar 

  • Odell, J., Caimi, P., Sauer, B. and Russell, S. (1990) Mol. Gen. Genet. 223, 369–378.

    Article  PubMed  CAS  Google Scholar 

  • Odell, J., Hoopes, J. and Vermerris, W. (1994) Plant Physiol. 106, 447–458.

    Article  PubMed  CAS  Google Scholar 

  • Oeller, P., Min-Wong, L., Taylor, L., Pike, D. and Theologis, A. (1991) Science 254, 437–439.

    Article  PubMed  CAS  Google Scholar 

  • Offringa, R., Franke-van Dijk, M., De Groot, M., van den Elzen, P. and Hooykaas, P. (1993) Proc. Natl. Acad Sci. USA 90, 7346–7350.

    Article  PubMed  CAS  Google Scholar 

  • Ow, D. and Medberry, S. (1995) Critical Reviews in Plant Sciences 14, 239–261.

    CAS  Google Scholar 

  • Poirier, Y., Dennis, D., Klomparens, K. and Somerville C. (1992) Science 256, 520–523.

    Article  PubMed  CAS  Google Scholar 

  • Puchta, H., Dujon, B. and Hohn, B. (1996) Proc. Natl. Acad. Sci. USA 93, 5055–5060.

    Article  PubMed  CAS  Google Scholar 

  • Register, J., Peterson, D., Bell, P., Bullock, W., Evans, I., Frame, B., Greenland, A., Higgs, N., Jepson, I., Jiao, S., Lewnau, C., Sillick, J. and Wilson, H. (1994) Plant Mol. Biol. 25, 951–961.

    Article  PubMed  CAS  Google Scholar 

  • Robson, P., McCormac, A., Irvine, A. and Smith, H. (1996) Nature Biotechnology 14, 995–998.

    Article  PubMed  CAS  Google Scholar 

  • Rugh, C., Wilde, H., Stack, N., Thompson, D., Summers, A. and Meagher, R. (1996) Proc. Natl. Acad. Sci. USA 93, 3182–3187.

    Article  PubMed  CAS  Google Scholar 

  • Saito, Y., Komari, T., Masuta, C., Hayashi, Y., Kumashiro, T. and Takanami, Y. (1992) Theor. Appl. Genet. 83, 679–683.

    Article  CAS  Google Scholar 

  • Shah, D., Horsch, R., Klee, H., Kishore, G., Winter, I., Turner, N., Hironaka, C., Sanders, P., Gasser, C., Aykent, S., Siegel, N., Rogers, S. and Fraley, R. (1986) Science 233, 476–481.

    Article  Google Scholar 

  • Stark, D., Barry, G., Muskopf, Y., Timmerman, K. and Kishore, G. (1991) Molecular Biology of Plant Growth and Development, Tucson, 714.

    Google Scholar 

  • Strittmatter, G., Janssens, J., Opsomer, C. and Botterman, J. (1995) Bio/Technology 13, 1085–1089.

    Article  CAS  Google Scholar 

  • Vaeck, M., Reynaerts, A., Höfte, H., Jansens, S., De Beuckeleer, M., Dean, C., Zabeau, M., Van Montagu, M. and Leemans, J. (1987) Nature 328, 33–37.

    Article  CAS  Google Scholar 

  • van der Salm, T., Hänisch ten Cate, C. and Dons, H. (1996) Plant Molecular Biology Reporter 14, 207–228.

    Article  Google Scholar 

  • Waiden, R., Fritze, K., Hayashi, H., Miklashevichs, E., Harling, H. and Schell, J. (1994) Plant Mol. Biol. 26, 1521–1528.

    Article  Google Scholar 

  • Walters, D., Vetsch, C., Potts, D., Lundquist, R. (1992) Plant. Mol. Biol. 18, 189–200.

    Article  PubMed  CAS  Google Scholar 

  • Zambryski, P., Joos, H., Genetello, C., Leemans, J., Van Montagu, M. and Schell, J. (1983) EMBO J. 4, 277–284.

    Google Scholar 

  • Zambryski, P. (1992) Annu. Rev. Plant Physiol. 43, 465–490.

    Article  CAS  Google Scholar 

Download references

Authors
  1. Kathleen D’Halluin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Johan Botterman
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Institute of Molecular Plant Sciences, Clusius Laboratory, Leiden University, Leiden, The Netherlands

    Herman P. Spaink  & Paul J. J. Hooykaas  & 

  2. Institut des Sciences Végétales, CNRS, Gif sur Yvette, Cedex, France

    Adam Kondorosi

Rights and permissions

Reprints and permissions

Copyright information

© 1998 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

D’Halluin, K., Botterman, J. (1998). The Use of Agrobacterium for Plant Genetic Engineering. In: Spaink, H.P., Kondorosi, A., Hooykaas, P.J.J. (eds) The Rhizobiaceae. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5060-6_17

Download citation

  • DOI: https://doi.org/10.1007/978-94-011-5060-6_17

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-0-7923-5180-1

  • Online ISBN: 978-94-011-5060-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation