Summary
A 15.2-kb KpnI fragment from the virulence region of pTiBo542, the Ti plasmid harbored by Agrobacterium tumefaciens strain A281, was introduced into binary vectors. The fragment contained the virB, virC and virG genes, and it is known to have the ability to increase the virulence of strains of A. tumefaciens. The strains of A. tumefaciens that carried the resulting plasmids were able to transform cells in a suspension culture of Chenopodium quinoa Willd cells which were not transformable by common vectors. Although the sizes of the plasmids was very large, a foreign segment of DNA was introduced into one of the plasmids by homologous recombination in A. tumefaciens cells, and the segment was subsequently transferred to plant cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- NPT:
-
neomycin phosphotransferase
- SPT:
-
streptomycin/spectinomycin phosphotransferase
References
An G (1985) Plant Physiol 79:568–570
An G, Evert PR, Mitra A, Ha SB (1988) In: Gelvin SB, Schilperoort RA (ed) Plant Molecular Biology Manual A3. Kluwer Academic, Dordrecht, pp 1–19
An G, Watson BD, Stachel S, Gordon MP, Nester EW (1985) EMBO J 4:277–284
Bevan MW, Chilton M-D (1982) Annu Rev Genet 16:357–384
Chilton M-D, Currier TC, Farrand SK, Bendich AJ, Gordon MP, Nester EW (1974) Proc Natl Acad Sci USA 71:3672–3676
DeGreve H, Decraemer H, Seurinck J, Van Montagu M, Schell J (1981) Plasmid 6:235–248
Depicker A, Van Montagu M, Schell J (1983) In: Kosuge T, Meridith CP, Hollaender A (ed) Genetic Engineering of Plants: an Agricultural Perspective. Plenum Publishing Corp, New York, pp. 143–176
Ditta G, Stanfield S, Corbin D, Helinski DR (1980) Proc Natl Acad Sci USA 77:7347–7351
Fraley RT, Rogers SG, Horsch RB (1986) CRC Crit Rev Plant Sci 4:1–46
Garfinkel DJ, Simpson RB, Ream LW, White FF, Gordon MP, Nester EW (1981) Cell 27:143–153
Herrera-Estrella L, Depicker A, Van Montagu M, Schell J (1983) Nature 303:209–213
Hoekema A, Hirsch PR, Hooykaas PJJ, Schilperoort RA (1983) Nature 303:179–180
Hood EE, Fraley RT, Chilton M-D (1987) Plant Physiol 83:529–534
Hood EE, Jen G, Kayes L, Kramer J, Fraley RT, Chilton M-D (1984) Bio/Technology 2:702–709
Horsch RB, Fraley RT, Rogers SG, Sanders PR, Lloyd A, Hoffmann N (1984) Science 223:496–498
Jin S, Komari T, Gordon MP, Nester EW (1987) J Bacteriol 169:4417–4425
Klee HJ, White FF, Iyer VN, Gordon MP, Nester EW (1983) J Bacteriol 153:878–883
Knauf V, Nester E (1982) Plasmid 8:45–54
Komari T (1989) Plant Science 60:223–229
Komari T, Halperin W, Nester EW (1986) J Bacteriol 166:88–94
Komari T, Saito Y, Nakakido F, Kumashiro T (1989) Theor Appl Genet 77:547–552
Linsmaier EM, Skoog F (1965) Physiol Plant 18:100–127
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular Cloning: a Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Montoya AL, Chilton M-D, Gordon MP, Sciaky D, Nester EW (1977) J Bacteriol 129:101–107
Nester EW, Gordon MP, Amasino RM, Yanofsky MF (1984) Annu Rev Plant Physiol 35:387–413
Ohshima Y, Suzuki Y (1977) Proc Natl Acad Sci USA 74:5363–5367
Tsujimoto Y, Suzuki Y (1979) Cell 16:425–436
Vietmeyer ND (1986) Science 232:1379–1384
Watson B, Currier TC, Gordon MP, Chilton M-D, Nester EW (1975) J Bacteriol 123:255–264.
Author information
Authors and Affiliations
Additional information
Communicated by A. R. Gould
Rights and permissions
About this article
Cite this article
Komari, T. Transformation of cultured cells of Chenopodium quinoa by binary vectors that carry a fragment of DNA from the virulence region of pTiBo542. Plant Cell Reports 9, 303–306 (1990). https://doi.org/10.1007/BF00232856
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00232856