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Use of comparative in situ hybridization and immunocytochemistry for the study of regulatory peptides

  • Giorgio Terenghi
  • Julia M. Polak
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  • 29 Downloads

Abstract

In the past decade immunocytochemistry has revolutionized the approach to morphological studies of tissues, allowing the localization of specific peptides and other cellular markers to be demonstrated. However, the knowledge gained by identifying cellular antigens is limited, as simple localization cannot give any information on the dynamic changes of cellular metabolic processes. The recent introduction of in situ hybridization for mRNA or DNA using labelled complementary nucleic acid probes has made possible the understanding of gene expression at the cellular level in different physiological and pathological conditions. Compared to other morphological and molecular biological techniques, in situ hybridization offers precise anatomical localization and increased sensitivity, opening completely new horizons to the scope of morphological studies.

Keywords

Dorsal Root Ganglion Atrial Natriuretic Peptide Ventral Horn Haematoxylin Counterstain Atrial Natriuretic Peptide Gene Expression 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media Dordrecht 1992

Authors and Affiliations

  • Giorgio Terenghi
    • 1
  • Julia M. Polak
    • 1
  1. 1.Histochemistry Department, Royal Postgraduate Medical SchoolHammersmith HospitalLondonUK

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