In Situ Hybridization: Medical Applications

  • G. R. Coulton
  • J. de Belleroche

Table of contents

  1. Front Matter
    Pages i-xii
  2. John McCafferty, Carl Alldus
    Pages 1-36
  3. Adrienne L. Morey, Kenneth A. Fleming
    Pages 66-96
  4. A. K. Raap, C. J. Cornelisse
    Pages 97-109
  5. Back Matter
    Pages 111-116

About this book


In situ hybridization has developed as a means of localizing specific DNA and RNA sequences within tissues. The great strength of this approach is the ability to relate the distribution of specific nucleic acids with cell structures and the protein products of the target gene by means of immunohistochemistry. Complementary DNA, RNA or oligonucleotide probes, suitably labelled, are hybridized to specific DNA or RNA targets within tissues. The spatial information thus obtained has contributed greatly to our understanding of such diverse areas of research as gene mapping, viral infection, cytogenetics, protein synthesis, prenatal diagnosis and tissue grafting.
This book is not intended as another recipe book, although it does describe theoretical and practical aspects of the technology. Rather, the authors critically describe the contribution made by in situ hybridization to specific areas of medical research.


Chromosom DNA genetics hybridization tissue

Editors and affiliations

  • G. R. Coulton
    • 1
  • J. de Belleroche
    • 1
  1. 1.Department of BiochemistryCharing Cross and Westminster Medical SchoolLondonUK

Bibliographic information