Abstract
Tobacco mesophyll protoplasts undergo a transition from G0- to S-phase upon culture on an appropriate medium containing auxin and cytokinin. In this process auxin plays a crucial role in inducing meristematic activity of differentiated and non-dividing cells. When we searched for genes which were induced upon the addition of auxin to the medium, three auxin-regulated genes; parA, parB and parC were isolated. Northern hybridization revealed that these genes were in fact expressed during the progression from G0- to S-phase. The predicted gene product of parA is proposed to play a role in transcriptional regulation, while that of parBencodes glutathione 5-transferase (GST). The GST is the first example, in which an auxin-regulated gene is ascribed to a specific enzymatic activity. In the promoter of parA gene, an auxin-responsive region has been identified. The significance of the expression of these genes and their gene products is discussed in relation to the molecular mode of auxin action.
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Nagata, T., Takahashi, Y. (1993). Auxin-mediated activation of DNA synthesis via par genes in tobacco mesophyll protoplasts. In: Ormrod, J.C., Francis, D. (eds) Molecular and Cell Biology of the Plant Cell Cycle. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-1789-0_10
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DOI: https://doi.org/10.1007/978-94-011-1789-0_10
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