Abstract
The 5′-upstream regions of 5 different carp α-globin genes(No. 3 to No. 7) and four virus promoters(SV40, RSV, LTR, and SRα) were linked with the reporter gene, chloramphenicol acetyltransferase(CAT) gene. The transcriptional activity of carp 5′-upstream regions and virus promoter regions were examined by CAT assay after transfection into fish non-erythroid cell lines, which were established from the ovary of hybrid tilapia(TO-2), fin cells of colored carp(GF), and human erythroleukemia cell lines(K562 and HEL cell). The CAT assay shows that these 5′-upstream regions from carp α-globin genes possess the promoter function in these cell lines. In the GF cell line, the CAT activity was significantly stronger than in TO-2, K562 and HEL cell lines. The CAT activities in fish cell lines directed by the SRα promoter were higher than that of other virus promoters.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Fu, L. and Aoki, T. (1991) ‘Expression of CAT gene directed by the 5′-upstream region from yellowtail a-globin gene in tissue cultured fish cells’, Nippon Suisan Gakkaishi 57, 1689–1696.
Fu, L. and Aoki, T. (1992) f Expression of carp α-globin gene in tilapia ovary cells’ in “Animal Cell Technology: Basic & Applied Aspects” (ed. by H. Murakami, S. Shirahata and H. Tachibana), Kluwer Academic publishers, Netherlands, 1992, pp.481–489.
Gong, Z., Hew, C. L., and Vielking, J. R. (1991) ‘Functional analysis and temporal expression of promoter region from fish antifreeze protein genes in transgenic Japanese medaka embryos’, Mol. Marine Bio. and Biotech. 1, 64–72.
Gorman, C. M., Moffat, L. F., and Howard, B. H. (1982) ‘Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells’, Mol. Cell. Bio. 2, 1044–1051.
Liu, Z., Moon, B., Faras, A. J., Guise, K. S., Kapuscinski, H. R., and Heckett, P. B. (1990) ‘Functional analysis of elements affecting expression of β-actin gene of carp’ Mol. Cell. Biol. 10, 3432–3440.
Lozzio, C. B. and Lozzio, R. B.(1975) ‘Human chronic myelogenous leukemia cell line with positive Philadelphia chromosome’, Blood 45, 321–334.
Martin, P. and Papayannopoulou, T.(1982) ‘A new human erythroleukemia cell line with spontaneous and induced globin expression’, Science, 216, 1233–1235.
Miyata, M., Hirono, I., and Aoki, T. (1993) ‘Analysis of a-globin gene family of carp. I. α-globin gene structure of No. 3, No. 6, and No. 7’, Nippon Suisan Gallaishi 59, 1077–1083.
Nobuta, K. and Aoki, T. (1992) ‘Cloning and complete nucleotide sequence of carp α-globin gene’, Nippon Suisan Gakkaishi 58, 49–54.
Sambrook, J., Fritsch, E. F., and Maniatis T. (1989) Molecular Cloning: a laboratory manual 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
Takebe, Y., Seiki, M., Fujisawa, J., Hoy, P., Yokota, K., Arai, K., Yoshida, M., and Arai, N. (1988) ‘SRα promoter: an efficient and versatile mammalian cDNA expres sion system composed of the simian virus 40 early promoter and R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat’, Mol.Cell.Biol. 8, 466–472.
Xiong, F., Chin, R. A., and Hew, C. L. (1992) ‘A gene encoding chinook salmon Onchorynchus tschawytscha prolactin: gene structure and potential cis-acting regulatory elements’, Mol. Marine Bio. and Biotech. 1, 155–164.
Yoshizaki, G., Kobayashi, S., Oshiro, T., and Takashima, F. (1992) ‘Introduction and expression of CAT gene in rainbow trout’, Nippon Suisan Gakkaishi 58, 1659–1665.
Zafarullah, M., Ronham, K., and Gedamu, L. (1988) ‘Structure of rainbowtrout metallothionein B gene and characterization of its metal-responsive region’, Mol. Cell. Biol. 8, 4469–4476.
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 1994 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Aoki, T., Fu, L., Hirono, I., Miyata, M. (1994). Functional Analysis of the Homology and Heterology Promoters in Fish-Cultured Cells. In: Kobayashi, T., Kitagawa, Y., Okumura, K. (eds) Animal Cell Technology: Basic & Applied Aspects. The Sixth International Meeting of Japanese Association for Animal Cell Technology JAACT’93, vol 6. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0848-5_20
Download citation
DOI: https://doi.org/10.1007/978-94-011-0848-5_20
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-4366-3
Online ISBN: 978-94-011-0848-5
eBook Packages: Springer Book Archive