Abstract
For studying the expression of the carp Cyprinus arcpio α-globin gene which contains the 3′- and 5′-flanking regions, 2 introns and 3 exons [1] in cultured fish cells, the carp α-globin gene was combined with the pSVO expression vector plasmid. This recombinant DNA was transfected into TO-2 cells derived from the ovary of the tilapia hybrid Tilapia mossambica X T. nilotica [2] by DNA-calcium phosphate coprecipitation. After transfection, the transcription of the introduced carp a-globin gene in TO-2 cells was analyzed. Results of dot-blot and Northern-blot hybridization of RNA showed that the carp α-globin gene had been successfully introduced and transcribed in the cultured fish cells. This means that the expression regulatory sequence of this gene can fulfill its function in the non-erythroid fish cells.
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References
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© 1992 Springer Science+Business Media Dordrecht
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Fu, L., Aoki, T. (1992). Expression of Carp α-Globin Gene in Tilapia Ovary Cells. In: Murakami, H., Shirahata, S., Tachibana, H. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2844-5_65
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DOI: https://doi.org/10.1007/978-94-011-2844-5_65
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