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Yeast artificial chromosome vectors

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Molecular and Cell Biology of Human Gene Therapeutics

Part of the book series: Molecular and Cell Biology of Human Diseases Series ((Mol. Cell Biol. Hu. Dis.,volume 20))

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Abstract

The ability to clone DNA fragments is vital to molecular biology. Until fairly recently the most commonly used vectors were plasmids, λ bacteriophage and cosmids (propagated in the bacterial host Escherichia coli), the latter of which could accommodate up to 50 kilobase pairs (kb) of DNA (Burke et al., 1987). The P1 based vectors doubled this maximum size to about 100 kb (Sternberg, 1990). However, many problems of eukaryote molecular biology, such as isolation of very large genes (e.g. the human dystrophin and cystic fibrosis genes), analysis of large transcription units, and physical mapping of ordered fragments from large genomes, require analysis of hundreds or even thousands of kb of contiguous DNA. The introduction of the yeast artificial chromosome (YAC) (Burke et al., 1987) as a cloning vehicle for fragments of DNA, that are at least one order of magnitude larger than was previously achieved using other systems, has opened these areas for investigation.

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Authors
  1. Stewart A. Fabb
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  2. Jiannis Ragoussis
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Editors and Affiliations

  1. Department of Biochemistry, Royal Holloway, University of London, Egham, Surrey, TW20 0EX, UK

    George Dickson

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© 1995 Springer Science+Business Media Dordrecht

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Fabb, S.A., Ragoussis, J. (1995). Yeast artificial chromosome vectors. In: Dickson, G. (eds) Molecular and Cell Biology of Human Gene Therapeutics. Molecular and Cell Biology of Human Diseases Series, vol 20. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0547-7_6

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  • DOI: https://doi.org/10.1007/978-94-011-0547-7_6

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-4242-0

  • Online ISBN: 978-94-011-0547-7

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