Abstract
Dominant selectable markers are an integral part of plant transformation strategies. A large number of such selectable marker genes have become available: antibiotic, antimetabolite, and herbicide resistance genes, hormone biosynthetic genes, and recently also genes conferring resistance to toxic levels of amino acids or amino acid analogs [19]. The usefulness of a particular resistance marker depends upon the characteristics of the selection agent, the resistance gene, and the plant material. The selection agent should fully inhibit growth of untransformed plant cells; however, the influence exerted by the dying, untransformed cells on the transformed cells should be minimal. Therefore, the lowest concentration of the selection agent that suppresses growth of untransformed cells is generally used. The sensitivity of plant cells to the selection agent depends upon the genotype, the explant type, the developmental stage, and the tissue culture conditions and should, therefore, be determined under the actual conditions of the transformation and regeneration process. Finally, the level of resistance also depends upon the transcriptional and translational control signals to which the resistance gene is fused. It may thus be necessary to test several gene constructions.
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© 1994 Springer Science+Business Media Dordrecht
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Angenon, G., Dillen, W., Van Montagu, M. (1994). Antibiotic resistance markers for plant transformation. In: Gelvin, S.B., Schilperoort, R.A. (eds) Plant Molecular Biology Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0511-8_9
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DOI: https://doi.org/10.1007/978-94-011-0511-8_9
Publisher Name: Springer, Dordrecht
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