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A chimaeric hygromycin resistance gene as a selectable marker in plant cells

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Summary

We show here that plant cells are sensitive to the antibiotic hygromycin-B4. We also show that a chimaeric gene consisting of the nopaline synthase (nos) gene regulatory elements and the E. coli derived hygromycin phosphotransferase (hpt) gene, when transferred to plants' cells, confers resistance to hygromycin B. The chimaeric nos-hpt gene enables efficient selection of DNA transfer to plant cells when used in conjunction with Ti plasmid-derived binary vectors in cocultivation experiments.

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Author notes

  1. Peter J. M. van den Elzen

    Present address: Dept. of Genetics, Free University, De Boelelaan 1087, 1081, Amsterdam, The Netherlands

Authors and Affiliations

  1. Advanced Genetic Sciences, Inc., 6701 San Pablo Avenue, 94608, Oakland, CA, U.S.A.

    Peter J. M. van den Elzen, Jeffrey Townsend, Kathleen Y. Lee & John R. Bedbrook

Authors
  1. Peter J. M. van den Elzen
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  2. Jeffrey Townsend
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  3. Kathleen Y. Lee
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  4. John R. Bedbrook
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van den Elzen, P.J.M., Townsend, J., Lee, K.Y. et al. A chimaeric hygromycin resistance gene as a selectable marker in plant cells. Plant Mol Biol 5, 299–302 (1985). https://doi.org/10.1007/BF00020627

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  • DOI: https://doi.org/10.1007/BF00020627

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