Abstract
Tumor specific human cytotoxic T lymphocytes (CTL) were induced by co-culturing peripheral blood mononuclear cells with X-ray-irradiated human lung squamous carcinoma cells, SQ-5, in the medium supplemented with interleukin (IL)-1, -2, -4 and -6, and 5% autologous plasma for 3 or 5 days. The CTL grew in serum/plasma-free mediurñ containing these four ILs and 0.5% bovine serum albumin for over a month and maintained killing activity of target cells within 48 hrs at an effector/target ratio of 1.25. Their growth was essentially dependent on the target SQ-5 cells, which were renewed every 5 days. When anti-CD3 monoclonal antibody was added to the serum/plasma-free medium supplemented with IL-1 and -2, the target tumor cells were not required to maintain the specific killing activity of the CTL. A large number of CTL (1011) was obtained in 35 days.
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Correspondence should be addressed to T. Ohno
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Liu, S.Q., Shiba, R., Soo, B.S., Saijo, K., Ohno, T. (1995). Long-Term Serum/Plasma-Free Culture of Human Cytotoxic T Lymphocytes Induced from Periphral Blood Mononuclear Cells. In: Beuvery, E.C., Griffiths, J.B., Zeijlemaker, W.P. (eds) Animal Cell Technology: Developments Towards the 21st Century. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0437-1_36
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DOI: https://doi.org/10.1007/978-94-011-0437-1_36
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