Abstract
A method of preparing two-dimensional surface spreads of plant synaptonemal complexes (SCs) associated with fluorescent in situ hybridization has been applied to analyze the location and organization of five different highly-repeated DNA sequences in rye. Our observations indicate that, depending on the type of sequence, the chromatin has different properties with regard to anchorage to the SC. While the organization of telomere sequences is maintained in different organisms, species seem to differ with respect to the organization of centromeric satellites.
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© 2002 Narosa Publishing House, New Delhi, India
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Cuñado, N., Barrios, J., Santos, J.L. (2002). Mapping and Organization of Highly-Repeated Sequences by Means of Fluorescence in situ Hybridization Against Synaptonemal Complex-Associated Chromatin of Rye. In: Sobit, R.C., Obe, G., Athwal, R.S. (eds) Some Aspects of Chromosome Structure and Functions. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0334-6_2
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DOI: https://doi.org/10.1007/978-94-010-0334-6_2
Publisher Name: Springer, Dordrecht
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