Summary
Two different strains, An 1 and An 2, were obtained from root nodules of Alnus nitida Endl., collected from one locality in the area of its natural habitat near Bahrin, District Swat, Pakistan. The light and electron microscopy of the isolates revealed the occurrence of septate and branched hyphae bearing sporangia and vesicles. The strains differed in their growth requirements, nitrogen-fixing ability and production of extracellular pigments, thus indicating the existence of more than one Frankia strain in the same locality. In the absence of combined nitrogen in the medium strain An 1 formed vesicles and fixed N2 (up to 200 nmol C2H4 .mg protein−1.h−1), while strain An 2 under the experimental conditions formed only few vesicles and fixed N2 at a very low rate (ca 10 nmol C2H4.mg protein−1.h−1). The nitrogenase activity of strain An 1 was strongly affected by the O, concentration. Frankia An 1 and An 2 were infective and effective on A. nitida and A. glutinosa but not on Datisca cannabina and Elaeagnus umbellata. Both An 1 and An 2 strains were more infective and effective on A. glutinosa than Frankia strains AvcIl and CpI1.
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© 1984 Martinus Nijhoff/Dr W. Junk Publishers, The Hague
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Hafeez, F., Akkermans, A.D.L., Chaudhary, A.H. (1984). Morphology, physiology and infectivity of two Frankia isolates An 1 and An 2 from root nodules of Alnus nitida . In: Akkermans, A.D.L., Baker, D., Huss-Danell, K., Tjepkema, J.D. (eds) Frankia Symbioses. Developments in Plant and Soil Sciences, vol 12. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-6158-6_6
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DOI: https://doi.org/10.1007/978-94-009-6158-6_6
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