Abstract
A critical review is given about the isolation and cultivation methods of Frankia species fromAlnus glutinosa root-nodules. The best results so far are obtained with a combination of sucrose (60% w/v)-sedimentation of root-nodule homogenate and subsequent suspension in the top-layer of a doubleagar layer system. The top-layer needs to contain a suitable C-source, in this study often a lipid factor from an alcoholic root-extract and an organic N-source.
The isolation and cultivation of Sp(−) and Sp(+) strains fromAlnus glutinosa root nodules and a Frankia from the root-nodules ofMyrica gale is reported. The regular observation of growing colonies appears to be very important for the interpretation of results. The latter was illustrated by the remarkable diauxic growth of the strains isolated fromAlnus glutinosa Sp(+) root nodules.
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Burggraaf, A.J.P., Quispel, A., Tak, T. et al. Methods of isolation and cultivation of Frankia species from actinorhizas. Plant Soil 61, 157–168 (1981). https://doi.org/10.1007/BF02277372
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DOI: https://doi.org/10.1007/BF02277372