Skip to main content
SpringerLink
Log in

Immunoassay Methods of Plant Hormone Analysis

  • Chapter
Plant Hormones and their Role in Plant Growth and Development

Abstract

There has been much recent interest in the application of immunoassays to the quantitation of plant hormones. Immunoassays are based on the ability of animals to produce proteins (antibodies) which recognize and bind to specific compounds (antigens) foreign to the animal. Such antibodies can be raised against plant hormones and used for hormone quantitation. The technique was first applied to plant hormones in an assay measuring the inactivation of hormone-labeled bacteriophage by antibodies raised against the hormone (12), but it has been radioimmunoassays (RIAs) and enzyme immunoassays (EIAs) which have been most widely used in this field (44).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Atzorn, R., Weiler, E. W. (1983) The immunoassay of gibberellins. I. Radioimunoassays for the gibberellins A1 A3, A4, A7, A9, and A20. Planta 159, 1–6.

    Article  CAS  Google Scholar 

  2. Atzorn, R., Weiler, E. W. (1983) The immunoassay of gibberellins. II. Quantitation of GA3, GA4, and GA7 by ultra-sensitive solid-phase enzyme immunoassays. Planta 159, 7–11.

    Article  CAS  Google Scholar 

  3. Atzorn, R., Weiler, E. W. (1983) The role of endogenous gibberellins in the formation of α-amylase by aleurone layers of germinating barley caryopses. Planta 159, 289–299.

    Article  CAS  Google Scholar 

  4. Badenoch-Jones, J., Letham, D. S., Parker, C.W., and Rolfe, B.G. (1984) Quantitation of cytokinins in biological samples using antibodies against zeatin riboside. Plant Physiol. 75, 1117–1125.

    Article  CAS  PubMed  Google Scholar 

  5. Caruso, J. L., Pence, V. C., Stroup, T. L., Nienaber, M. A. (1985) Comparison of enzyme- linked immunosorbent assay (ELISA) and gas chromatography-mass spectrometry for the quantitation of indole-3-acetic acid. Plant Physiol. Suppl. 77, 2.

    Google Scholar 

  6. Constantinidou, H. A., Steele, J. A., Kozlowski, T. T., Upper, C. D. (1978) Binding specificity and possible analytical applications of the cytokinin-binding antibody, anti- N6-benzyladenosine. Plant Physiol. 62, 968–974.

    Article  CAS  PubMed  Google Scholar 

  7. Crozier, A., Sandberg, G., Monteiro, A. M., Sundberg, B. (1986) The use of immunological techniques in plant hormone analysis. In: Plant growth substances 1985. pp. 13–21, Bopp. M. ed. Berlin, Springer-Verlag.

    Google Scholar 

  8. Daie, J., Wyse, R. (1982) Adaptation of the enzyme-linked immunosorbent assay (ELISA) to the quantitative analysis of abscisic acid. Anal. Biochem. 119, 365–371.

    Article  CAS  PubMed  Google Scholar 

  9. Davis, G. C., Hein, M. B., Chapman, D. A., Neely, B. C., Sharp, C. R., Durley, R. C., Biest, D. K., Heyde, B. R., Carnes, M. G. (1986) Immunoaffinity columns for the isolation and analysis of plant hormones. In: Plant growth substances 1985. pp. 44–51, Bopp, M. ed. Berlin, Springer-Verlag.

    Google Scholar 

  10. Ernst, D., Schafer, W., Oesterhelt, D. (1983) Isolation and quantitation of isopentenyladenosine in an anise cell culture by single-ion monitoring, radioimmunoassay and bioassay. Planta 159, 216–221.

    Article  CAS  Google Scholar 

  11. Finkelstein, R. R., Tenbarge, K. M., Shumway, J. E., Crouch, M. L. (1985) Role of ABA in maturation of rapeseed embryos. Plant Physiol. 78, 630–636.

    Article  CAS  PubMed  Google Scholar 

  12. Fuchs, S., Haimovich, J., Fuchs, Y. (1971) Immunological studies of plant hormones. Detection and estimation by immunological assays. Eur. J. Biochem. 18, 384–390.

    Article  CAS  PubMed  Google Scholar 

  13. Hansen, C. E., Wenzler, H., Meins, F., Jr. (1984) Concentration gradients of trans-zeatin riboside and trans-zeatin in the maize stem. Plant Physiol. 75, 959–963.

    Article  CAS  PubMed  Google Scholar 

  14. Heineman, W. R., Halsell, H. B. (1985) Strategies of electrochemical immunoassay. Analytical Chem. 57, 1321A–1331A.

    CAS  Google Scholar 

  15. Jones, H. G. (1985) Correction for non-specific interference in hormone immunoassay. 12th International Conference on Plant Growth Substances, Heidelberg, Abstract no. 1105.

    Google Scholar 

  16. Khan, S. A., Humayun, M. Z., Jacob, T. M. (1977) A sensitive radioimmunoassay for isopentenyladenosine. Anal. Biochem. 83, 632–635.

    Article  CAS  PubMed  Google Scholar 

  17. LePage-Degivry, M. T., Duval, D., Bulard, C., Delaage, M. (1984) A radioimmunoassay for abscisic acid. J. Immun. Methods 67, 119–128.

    Article  CAS  Google Scholar 

  18. MacDonald, E. M. S., Morris, R. O. (1985) Isolation of cytokinins by immunoaffinity chromatography and analysis by high-performance liquid chromatography radioimmunoassay. In: Methods in Enzymology, vol. 110. Steroids and isoprenoids, Part A. pp. 347–358, Law, J. H., Rilling, H. C., eds. New York, Academic Press.

    Google Scholar 

  19. Madej, A., Haggblom, P. (1985) Radioimmunoassay for determination of indole-3-acetic acid in fungi and plants. Physiol. Plant. 64, 389–392.

    Article  CAS  Google Scholar 

  20. Maggio, E. T. (1980) Enzymes as immunochemical labels. In Enzyme-immunoassay, pp. 53–70, Maggio, E.T., ed. Boca Raton, Florida, CRC Press, Inc.

    Google Scholar 

  21. Mertens, R., Deus-Neumann, B., Weiler, E. W. (1983) Monoclonal antibodies for the detection and quantitation of the endogenous growth regulator, abscisic acid. FEBS Lett. 160, 269–272

    Article  CAS  Google Scholar 

  22. Mertens, R., Eberle, J., Arnscheidt, A., Ledebur, A., Weiler, E. W. (1985) Monoclonal antibodies to plant growth regulators. II. Indole-3-acetic acid. Planta 166, 389–393.

    Article  CAS  Google Scholar 

  23. Morris, R. O., Akiyoshi, D. E., MacDonald, E. M. S., Morris, J. W., Regier, D. A., Zaerr, J. B. (1982) Cytokinin metabolism in relation to tumor induction by Agrobacterium tumefaciens. In: Plant growth substances, pp. 175–183, Wareing, P. F. ed. New York, Academic Press.

    Google Scholar 

  24. Pence, V. C., Caruso, J. L. (1986) Auxin and cytokinin levels in selected and temperature-induced morphologically distinct tissue lines of tobacco crown gall tumors. Plant Science (in press)

    Google Scholar 

  25. Pence, V. C., Caruso, J. L. (1986) ELISA determination of IAA using antibodies against ring-linked IAA. Phytochemistry (in press).

    Google Scholar 

  26. Pengelly, W. L. (1986) Validation of immunoassays. In: Plant growth substances 1985. pp. 35–43, Bopp, M. ed. Berlin, Springer-Verlag.

    Google Scholar 

  27. Pengelly, W. L., Bandurski, R. S., Shulze, A. (1981) Validation of a radioimmunoassay for indole-3-acetic acid using gas chromatography-selected ion monitoring-mass spectrometry. Plant Physiol. 68, 96–98.

    Article  CAS  PubMed  Google Scholar 

  28. Pengelly, W., Meins, F., Jr. (1977) A specific radioimmunoassay for nanogram quantities of the auxin, indole-3-acetic acid. Planta 136, 173–180.

    Article  CAS  Google Scholar 

  29. Raikhel, N. V., Hughes, D. W., Galau, G. A. (1986) An enzyme-immunoassay for quantitative analysis of abscisic acid in wheat. In: Molecular Biology of Plant Growth Control, Fox, J. E., Jacobs, M., eds. New York, Alan R. Liss, Inc. (in press).

    Google Scholar 

  30. Reeve, D. R., Crozier, A. (1980) Quantitative analysis of plant hormones. In: Encyclopedia of plant physiology, new series, Vol. 9, pp. 203–280, MacMillan, J., ed. Heidelberg, Springer-Verlag.

    Google Scholar 

  31. Rosher, P. H., Jones, H. G., Hedden, P. (1985) Validation of a radioimmunoassay for (+)- abscisic acid in extracts of apple and sweet-pepper tissue using high-pressure liquid chromatography and combined gas chromatography mass spectrometry. Planta 165, 9199.

    Article  Google Scholar 

  32. Sandberg, G, Ljung, K., Aim, P. (1985) Precision and accuracy of radioimmunoassay in the analysis of endogenous 3-indoleacetic acid from needles of Scots pine. Phytochem. 24, 1439–1442.

    Article  CAS  Google Scholar 

  33. Siddle, K. (1985) Properties and application of monoclonal antibodies. In: Alternative immunoassays, pp. 13–37, Collins, W. P. ed. New York, John Wiley & Sons.

    Google Scholar 

  34. Sotta, B., Sossountzov, L., Maldiney, R., Sabbagh, I., Tachon, P., Miginiac, E. (1985) Abscisic acid localization by light microscopic immunochemistry in Chenopodium polyspermum L. J. Histochem. Cytochem. 33, 201–208.

    Article  CAS  PubMed  Google Scholar 

  35. Vold, B. S., Leonard, N. J. (1981) Production and characterization of antibodies and establishment of a radioimmunoassay for ribosylzeatin. Plant Physiol. 67, 401–403.

    Article  CAS  PubMed  Google Scholar 

  36. Walton, D., Dashek, W., Galson, E. (1979) A radioimmunoassay for abscisic acid. Planta 146, 139–145.

    Article  CAS  Google Scholar 

  37. Wang, T. L., Griggs, P., Cook, S. (1986) Immunoassays for plant growth regulators — a help or a hindrance? In: Plant growth substances 1985. pp. 26–34, Bopp, M. ed. Berlin, Springer-Verlag.

    Google Scholar 

  38. Weiler E. W. (1979) Radioimmunoassay for the determination of free and conjugated abscisic acid. Planta 144, 255–263.

    Article  CAS  Google Scholar 

  39. Weiler, E. W. (1980) Radioimmunoassays for the differential and direct analysis of free and conjugated abscisic acid in plant extracts. Planta 148, 262–272.

    Article  CAS  Google Scholar 

  40. Weiler, E. W., (1980) Radioimmunoassays for trans-zeatin and related cytokinins. Planta 149, 155–162.

    Article  CAS  Google Scholar 

  41. Weiler, E. W. (1981) Radioimmunoassay for pmol-quantities of indole-3-acetic acid in plant extracts. Planta 153, 310–325.

    Google Scholar 

  42. Weiler, E. W. (1982) An enzyme-immunoassay for cis-(+)- abscisic acid. Physiol. Plant. 54, 510–514.

    Article  CAS  Google Scholar 

  43. Weiler, E. W. (1984) Immunoassay of plant growth regulators. Annu. Rev. Plant Physiol. 35, 85–95.

    Article  CAS  Google Scholar 

  44. Weiler, E. W., Eberle, J., Mertens, R., Atzorn, R., Feyerabend, M., Jourdan, P. S., Arnscheidt, A., Wieczorek, U. (1986) Antisera- and monoclonal antibody based immunoassay of plant hormones. In: Immunology in plant science, pp. 27–58, Wang, T. L., ed. Cambridge, Cambridge University Press.

    Google Scholar 

  45. Weiler, E. W., Jourdan, P. S., Conrad, W. (1981) Levels of indole-3-acetic acid in intact and decapitated coleoptiles as determined by a specific and highly sensitive solid-phase enzyme-immunoassay. Planta 153, 561–571.

    Article  CAS  Google Scholar 

  46. Weiler, E. W., Wieczorek, U. (1981) Determination of femtomol quantities of gibberellic acid by radioimmunoassay. Planta 152, 159–167.

    Article  CAS  Google Scholar 

  47. Woodside, M. L., Latimer, L. J. P., Janzer, J. J., McLennan, B. D., Lee, J. S. (1983) Characterization of monoclonal antibodies specific for isopentenyl adenosine derivatives occurring in transfer RNA. Biochem. Biophys. Res. Commun. 114, 791–796.

    Article  Google Scholar 

  48. Zavala, M. E., Brandon, D. L. (1983) Localization of a phytohormone using immunocytochemistry. J. Cell Biol. 97, 1235–1239.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biological Sciences, University of Cincinnati, Cincinnati, Ohio, 45221, USA

    Valerie C. Pence & John L. Caruso

Authors
  1. Valerie C. Pence
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. John L. Caruso
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Section of Plant Biology New York State College of Agriculture and Life Sciences, Cornell University, Ithaca, New York, USA

    Peter J. Davies

Rights and permissions

Reprints and permissions

Copyright information

© 1987 Martinus Nijhoff Publishers, Dordrecht

About this chapter

Cite this chapter

Pence, V.C., Caruso, J.L. (1987). Immunoassay Methods of Plant Hormone Analysis. In: Davies, P.J. (eds) Plant Hormones and their Role in Plant Growth and Development. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-3585-3_13

Download citation

  • DOI: https://doi.org/10.1007/978-94-009-3585-3_13

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-247-3498-6

  • Online ISBN: 978-94-009-3585-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation