Abstract
Two applications using anthraquinone dyes for protein separation and purification are presented. In both cases, Cibacron Blue F3GA is attached to water-soluble polyvinyl alcohol) to facilitate a homogeneous binding to the protein. To effect precipitation, the poly-dye’s solubility can be altered either via binding to a multimeric protein, or via independent control of the polymer solubility. In the case of ultrafiltration, the poly-dye provides sufficient size to prevent the target protein from entering the UF membrane. These early results indicate that PVA is a good polymer for UF, but that the precipitation poly-dye is not correctly formulated.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
C.R. Lowe, D.A.P. Small, and A. Atkinson, Int. J. Biochem. 13, 33 (1981).
A.S. Michaels, Ultrafiltration, in E.S. Perrry (ed.), Advances in Separation and Purification. John Wiley, New York (1968).
K.B. Male, J.H.T. Luong, and A.L. Nguyen, Enzyme Microb. Technol. 9, 374 (1987).
B. Mattiasson and M. Ramstorp, Ann. N.Y. Acad. Sci. 413, 307 (1983).
T.B. Choe, P. Masse, and A. Verdier. Biotechnol. Lett. 8.163 (1986).
E. Pungor, Jr., N.B. Afeyan, N.F. Gordon, and C.L. Cooney, Bio/Technology 5, 604 (1987).
J. Bonnerjea, J. Jackson, M. Hoare, P. Dunnill, Enzyme Microb. Technol. 10, 357 (1988).
P.-O. Larsson, S. Flygare, and K. Mosbach, Meth. Enzymology 104, 364 (1984).
M. Hayet and M.A. Vijayalakshmi, J. Chromatogr. 376. 157 (1986).
M. Schneider, C. Guillot, and B. Lamy, Ann. N.Y. Acad. Sci. 369, 257 (1981).
A.S. Hoffman, K.A. Auditore-Hargreaves, C.A. Cole, R.L. Houghton, N. Monji, R.C. Nowinski, J.B. Plastino, and J.H. Priest, “Two novel applications of polymer chemistry and physics for immunoassays and bioseparations,” in Proc. of International Meeting on Polymers in Medicine and Surgery V (The Netherlands, September 10–12, 1986).
R.R. Fisher and C.E. Glatz, “Polyelectrolyte precipitation of proteins. II. Effects of the reactor conditions,” Biotechnol. Bioeng. (to appear).
R.S. Beissner and F.B. Rudolph, Arch. Biochem. Biophvs. 189, 76 (1978).
S. Subramanian, Arch. Biochem. Biophvs. 216, 116 (1982).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Elsevier Science Publishers LTD
About this chapter
Cite this chapter
Fisher, R.R., Machiels, B., Kyriacou, K.C., Morris, J.E. (1989). Affinity Ultrafiltration and Affinity Precipitation Using a Water-Soluble Complex of Poly(vinyl Alcohol) and Cibacron Blue F3GA. In: Vijayalakshmi, M.A., Bertrand, O. (eds) Protein-Dye Interactions: Developments and Applications. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1107-9_20
Download citation
DOI: https://doi.org/10.1007/978-94-009-1107-9_20
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6989-2
Online ISBN: 978-94-009-1107-9
eBook Packages: Springer Book Archive