Abstract
An analytical procedure based on isoelectric focusing was developed to identify contaminants in a nuclease preparation. Two categories of contaminants were visualized: protein impurities from the process background and modified forms of the enzyme. The potential of the analysis was tested on a fermentation with E. coli producing and secreting the enzyme, and using different purification procedures. Introduction of simple analyses to validate quality of a product at any stage in the developmental phase of a production can help to outline a production strategy.
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Biedermann, K., Jepsen, P.K., Pedersen, J., Nielsen, B.R. (1990). Analyses of Purity and Homogeneity in Production Processes. In: Pyle, D.L. (eds) Separations for Biotechnology 2. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0783-6_49
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DOI: https://doi.org/10.1007/978-94-009-0783-6_49
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6839-0
Online ISBN: 978-94-009-0783-6
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