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Release of periplasmic enzymes fromEscherichia coli using tangential flow filtration

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Biotechnology Techniques

Summary

Escherichia coli cells producing the periplasmic enzymes nuclease, β-lactamase and alkaline phosphatase were exposed to osmotic shock treatment in a closed system consisting of a tank, a pump and a filtration unit. The enzymes were released by circulating the cell suspensions in the filtration system and separated from cells and spheroplasts by filtration. This novel releasing method was shown to be equally effective as osmotic shock treatments performed by agitation and centrifugation. Since the extraction is performed in a closed system, aerosol formation is avoided. In addition the method may easily be scaled up.

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Authors and Affiliations

  1. Department of Biotechnology, The Technical University of Denmark, Block 223, DK-2800, Lyngby

    Kirsten Biedermann & Pia Knak Jepsen

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  1. Kirsten Biedermann
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  2. Pia Knak Jepsen
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Biedermann, K., Jepsen, P.K. Release of periplasmic enzymes fromEscherichia coli using tangential flow filtration. Biotechnol Tech 3, 39–44 (1989). https://doi.org/10.1007/BF01876219

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