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Engineered Zinc Finger Nucleases for Targeted Genome Editing

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Site-directed insertion of transgenes

Part of the book series: Topics in Current Genetics ((TCG,volume 23))

Abstract

Zinc finger nucleases (ZFNs) are artificial proteins consisting of engineered zinc finger proteins fused to the FokI endonuclease domain. These nucleases bind to specific DNA recognition sites and introduce double-strand breaks (DSBs). Repair of these DSBs by normal cellular processes can be exploited to either disrupt genes or significantly increase the frequency of homologous recombination with a user-defined repair template. Several platforms have been developed that enable engineering of zinc finger proteins that bind with high affinity and specificity to 9–18 bp target DNA sequences. ZFNs have already been used in basic research, most prominently with the development of methods to efficiently modify various model organisms and cell lines.

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Acknowledgements

We thank members of the Joung lab for helpful comments and suggestions. We thank Victor Quesada for assistance with the development of Fig. 5.1. C.L.R. is partially supported by the National Science Foundation and the Ford Foundation. J.K.J is supported by National Institutes of Health (NIH) R01 GM088040, an NIH Director’s Pioneer Award (DP1 OD006862), and the Massachusetts General Hospital Jim and Ann Orr Research Scholar Award.

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    Cherie L. Ramirez & J. Keith Joung

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Ramirez, C.L., Joung, J.K. (2013). Engineered Zinc Finger Nucleases for Targeted Genome Editing. In: Renault, S., Duchateau, P. (eds) Site-directed insertion of transgenes. Topics in Current Genetics, vol 23. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4531-5_5

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