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Understanding Epigenetic Memory is the Key to Successful Reprogramming

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Induced Pluripotent Stem Cells in Brain Diseases

Part of the book series: SpringerBriefs in Neuroscience ((BRIEFSNEUROSCI))

Abstract

Molecular biologists have developed powerful tools for measuring and/or describing a cell’s epigenome, including: bisulfite-sequencing, chromatin-immunoprecipitation (ChIP), and comprehensive high-throughput arrays for relative methylation (CHARM). Over the last few years, a body of work has emerged using such techniques to map the entire epigenetic landscape of ESCs, iPSCs, and terminally differentiated cells. In this way the underlying mechanisms of differentiation, as well as the effects of artificial de-, re-, and trans-differentiation can be understood by comparing important differences in their epigenetic states.

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Author notes

  1. Judith Wagner

    Present address: Adolf-Butenandt-Institute, Biochemistry, Ludwig-Maximilians-University, 80336, Munich, Germany

Authors and Affiliations

  1. Center for Children with White Matter Disorders, VU University Medical Center, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands

    Vivi M. Heine, Stephanie Dooves & Dwayne Holmes

  2. Clinical Genetics, VU University Medical Center, Van der Boechorststraat 7, 1081 BT, Amsterdam, The Netherlands

    Judith Wagner

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  1. Vivi M. Heine
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  2. Stephanie Dooves
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  3. Dwayne Holmes
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  4. Judith Wagner
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Correspondence to Vivi M. Heine .

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Heine, V.M., Dooves, S., Holmes, D., Wagner, J. (2012). Understanding Epigenetic Memory is the Key to Successful Reprogramming. In: Induced Pluripotent Stem Cells in Brain Diseases. SpringerBriefs in Neuroscience. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-2816-5_2

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