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MicroRNAs as Potential Engineering Targets for Improvement of CHO Cell Production Phenotypes

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Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009

Part of the book series: ESACT Proceedings ((ESACT,volume 5))

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Abstract

MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression. They have been implicated in diverse cellular functions and are currently the subject of considerable interest in all aspects of cell biology. They are highly conserved evolutionarily – the first published hamster sequence (cgr-miR-21) was found to be identical to human, mouse and rat.

In this study, we discuss the identification of several differentially expressed miRNAs after shifting CHO cells from exponential growth at 37°C to growth arrest at 31°C (temperature shift). Our data suggest that these miRNAs represent attractive targets for engineering a culture process from growth phase to production phase, thereby potentially replacing or enhancing the use of temperature-shift.

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Authors and Affiliations

  1. National Institute for Cellular Biotechnology, Dublin City University, Dublin, Ireland

    Niall Barron, Niraj Kumar, Patrick Gammell & Martin Clynes

  2. National Institute for Cellular Biotechnology, Dublin City University, Dublin 9, Ireland

    Noelia Sanchez

  3. Bio-Manufacturing Sciences Group, Pfizer, Inc., Grange Castle International Business Park, Clondalkin, Dublin, Ireland

    Patrick Gammell

Authors
  1. Niall Barron
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  2. Niraj Kumar
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  3. Noelia Sanchez
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  4. Patrick Gammell
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  5. Martin Clynes
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Corresponding author

Correspondence to Niall Barron .

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Editors and Affiliations

  1. , University College Dublin, National Institute of Bioprocessing Rese, Engineering Building, Belfield, Dublin, Ireland

    Nigel Jenkins

  2. , National Institute for Cellular Biotechn, Dublin City University, Dublin, Ireland

    Niall Barron

  3. Tecnologica (IBET), ITQB, Instituto de Biologia Experimental e, Oeiras, Portugal

    Paula Alves

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© 2012 Springer Science+Business Media B.V.

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Barron, N., Kumar, N., Sanchez, N., Gammell, P., Clynes, M. (2012). MicroRNAs as Potential Engineering Targets for Improvement of CHO Cell Production Phenotypes. In: Jenkins, N., Barron, N., Alves, P. (eds) Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009. ESACT Proceedings, vol 5. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-0884-6_1

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