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Quantitative Analysis of Lipid Peroxidation Products Using Mass Spectrometry

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Sample Preparation in Biological Mass Spectrometry

Abstract

It is important to assess the oxidative injury in vivo accurately and inclusively, as the oxidative stress induced by various oxidants in a random and destructive fashion is considered to play an important role in the pathophysiology of a number of human disorders. A method for the measurement of lipid peroxidation products in vivo, such as hydroxyoctadecadienoic acids (HODE) and 7-hydroxycholesterol (OHCh) determined by GC-MS and LC-MS/MS, is presented in this chapter.

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Correspondence to Yasukazu Yoshida .

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Yoshida, Y., Shichiri, M. (2011). Quantitative Analysis of Lipid Peroxidation Products Using Mass Spectrometry. In: Ivanov, A., Lazarev, A. (eds) Sample Preparation in Biological Mass Spectrometry. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-0828-0_42

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