Sample Preparation in Biological Mass Spectrometry

  • Alexander R. Ivanov
  • Alexander V. Lazarev

Table of contents

  1. Front Matter
    Pages i-xxix
  2. Traditional and Improved Techniques in Sample Preparation for Proteomics

    1. Front Matter
      Pages 1-1
    2. Xianyin Lai, Frank A. Witzmann
      Pages 3-17
    3. David Arnott, Peter S. Liu, Patricia Molina, Lilian Phu, Wendy N. Sandoval
      Pages 19-40
    4. Judit M. Nagy, Alexandria Lipka, Fiona Pereira, Nicky Marlin, Stuart Hassard
      Pages 41-60
    5. Emily A. Freeman, Alexander R. Ivanov
      Pages 77-90
  3. Methods for Improved Proteolytic Digestion

    1. Front Matter
      Pages 107-107
    2. Matthew J. Berberich, Jeffrey A. Kowalak, Anthony J. Makusky, Brian Martin, Detlef Vullhorst, Andres Buonanno et al.
      Pages 109-124
    3. Daniel Lopez-Ferrer, Kim K. Hixson, Mikhail E. Belov, Richard D. Smith
      Pages 125-139
  4. Methods for Tissue, Cell, and Organelle Preparation and Analysis

    1. Front Matter
      Pages 157-157
    2. Diem Tran, Mark Daniels, Ben Colson, Dikran Aivazian, Antonio Boccia, Ingrid Joseph et al.
      Pages 159-170
    3. Yoshinori Masuo, Misato Hirano, Junko Shibato, Hyung Wook Nam, Isabelle Fournier, Céline Mériaux et al.
      Pages 171-195
    4. WenKui Lan, Marc J. Horn, Fumihiko Urano, Andre Kopoyan, Sun W. Tam
      Pages 197-214
  5. 2D Gel-Based Proteomics

  6. Sample Preparation and Analysis Techniques for Biological Fluids and Biomarker Discovery

    1. Front Matter
      Pages 267-267
    2. Craig A. Gelfand, Gilbert S. Omenn
      Pages 269-289
    3. Wasfi Alrawashdeh, Tatjana Crnogorac-Jurcevic
      Pages 291-326
    4. Joao A. Paulo, Ali R. Vaezzadeh, Darwin L. Conwell, Richard S. Lee, Hanno Steen
      Pages 327-360
  7. Sample Preparation Methods in Plant Proteomics

    1. Front Matter
      Pages 361-361
    2. Laurence V. Bindschedler, Celia J. Smith, Rainer Cramer
      Pages 363-380
    3. Olga A. Koroleva, Laurence V. Bindschedler
      Pages 381-409
    4. Anders Laurell Blom Møller, Katja Witzel, Annelies Vertommen, Vibeke Barkholt, Birte Svensson, Sebastien Carpentier et al.
      Pages 411-434
  8. Affinity Interaction and Biochemical Enrichment Techniques

    1. Front Matter
      Pages 435-435
    2. Guillaume O. Adelmant, Job D. Cardoza, Scott B. Ficarro, Timothy W. Sikorski, Yi Zhang, Jarrod A. Marto
      Pages 437-486
    3. Dean E. McNulty, Michael J. Huddleston, Roland S. Annan
      Pages 487-495
    4. Diarmuid T. Kenny, Liaqat Ali, Samah Issa, Niclas G. Karlsson
      Pages 497-513
    5. Terry Zhang, Rosa Viner, Zhiqi Hao, Vlad Zabrouskov
      Pages 535-546
  9. Methods for Quantitative Proteomics

    1. Front Matter
      Pages 547-547
    2. Keith Ashman, María Isabel Ruppen Cañás, Jose L. Luque-Garcia, Fernando García Martínez
      Pages 549-573
    3. Leanne B. Ohlund, Darryl B. Hardie, Monica H. Elliott, Alexander G. Camenzind, Derek S. Smith, Jennifer D. Reid et al.
      Pages 575-624
  10. Sample Preparation in Functional Proteomics

    1. Front Matter
      Pages 625-625
    2. Emily A. Freeman, Vera Gross, Ilyana Romanovsky, Alexander V. Lazarev, Alexander R. Ivanov
      Pages 657-670
    3. Mari Enoksson, Miklós Békés, Laurence M. Brill, Khatereh Motamedchaboki
      Pages 671-687
  11. Characterization of Membrane Proteins

    1. Front Matter
      Pages 689-689
    2. Eric Bonneil, Sylvain Brunet, Michel Jaquinod, Joseph P.M. Hui, Anik Forest, Pierre Thibault
      Pages 691-714
  12. Methods for MALDI MS Enabled Biomedical Applications

    1. Front Matter
      Pages 731-731
    2. Ali Tiss, Celia J. Smith, Rainer Cramer
      Pages 733-747
    3. Appavu K. Sundaram, Berk Oktem, Jane Razumovskaya, Shelley N. Jackson, Amina S. Woods, Vladimir M. Doroshenko
      Pages 749-764
    4. Isabelle Fournier, Céline Mériaux, Maxence Wisztorski, Randeep Rakwal, Michel Salzet
      Pages 765-783
  13. Clinical Research and Applications

    1. Front Matter
      Pages 811-811

About this book


The goal and philosophy of sample preparation in biological mass spectrometry is to reveal the actual multicomponent molecular structure of a biological specimen that can be assessed by mass spectrometry tools. Unfortunately, sample preparation is often looked at as a necessary but “boring” step which does not warrant any efforts beyond a paragraph or two in a “Methods” section; it is believed not to be conducive to innovation, and does not elicit the enthusiasm of funding agencies. Nevertheless, in recent years, the attention to sample preparation methods has risen considerably, both in Academia and in the life science industry. The aim of this book is to provide the researcher with important sample preparation strategies for a wide variety of analyte molecules, specimens, methods, and biological applications demanding mass spectrometric analysis as the detection end-point. In this volume we have compiled contributions from several laboratories that employ mass spectrometry for biological analysis.

With the latest inventions and the introduction of highly sophisticated mass spectrometry equipment, sample preparation has become an extremely important bottleneck of biomedical analysis. This book is structured as a compilation of contributed chapters ranging from step-by-step protocols to research articles and reviews. The main philosophy of this volume is that sample preparation methods have to be optimized and validated for every project, for every sample type and for every downstream analytical technique.


Analytical biochemistry Mass spectrometry Proteomics and protein chemistry Protocols and novel techniques Sample preparation

Editors and affiliations

  • Alexander R. Ivanov
    • 1
  • Alexander V. Lazarev
    • 2
  1. 1., Department of Genetics and Complex DiseaHarvard School of Public Health, HarvardBostonUSA
  2. 2., Research and DevelopmentPressure BioSciences, Inc.South EastonUSA

Bibliographic information