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Preanalytical Variables for Plasma and Serum Proteome Analyses

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Sample Preparation in Biological Mass Spectrometry

Abstract

Proteomic biomarker discovery, confirmation, and validation studies depend absolutely on great care in the management and documentation of preanalytical sources of variation. This consideration is particularly important for such complex and dynamic specimens as plasma. Clues to the awareness of the investigator are clarity about whether the specimen is serum or plasma, reasons stated for the choice, specification of the anticoagulant in the case of plasma, and statement about whether enrichment, depletion, or protease inhibition methods were deployed. This chapter provides a systematic discussion of preanalytical variables affecting plasma and serum and offers practical guidance for reducing undesirable variation in results from these sources.

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Authors and Affiliations

  1. BD Diagnostics, Preanalytical Systems, Franklin Lakes, NJ, 07417, USA

    Craig A. Gelfand

  2. Center for Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI, 48109-2218, USA

    Gilbert S. Omenn

Authors
  1. Craig A. Gelfand
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  2. Gilbert S. Omenn
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Correspondence to Craig A. Gelfand .

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Editors and Affiliations

  1. , Department of Genetics and Complex Disea, Harvard School of Public Health, Harvard, Huntington Ave., SPH-1 665, Boston, 02115, Massachusetts, USA

    Alexander R. Ivanov

  2. , Research and Development, Pressure BioSciences, Inc., Norfolk Ave. 14, South Easton, 02375, Massachusetts, USA

    Alexander V. Lazarev

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Gelfand, C.A., Omenn, G.S. (2011). Preanalytical Variables for Plasma and Serum Proteome Analyses. In: Ivanov, A., Lazarev, A. (eds) Sample Preparation in Biological Mass Spectrometry. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-0828-0_16

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