Abstract
Today’s scenario is nanoscience and nanotechnology performing significant contributions of protein nanoparticles (PNPs) for delivery of various life savings biomolecules. Among the various PNPs, gelatin nanoparticles (GNPs) exhibit good capabilities for biomedical application because of their biodegradability, biocompatibility, non-toxicities and its cost-effectiveness. In this chapter, we have discussed different methods for GNPs preparation and their functionalization for target delivery of drugs and genes for a range of diseases like cancer, malarial and infectious. We also discussed ocular, pulmonary drugs as well as nutraceutical, proteins and peptides delivery. We also come across their application in tissue engineering.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
N.M. Molino, S.W. Wang, Caged protein nanoparticles for drug delivery. Curr. Opin. Biotechnol. 28, 75–82 (2014)
W. Lohcharoenkal, L. Wang, Y.C. Chen, Y. Rojanasakul, Protein nanoparticles as drug delivery carriers for cancer therapy. Biomed. Res. Int. 2014, 12 (2014)
K.D. Schwenke, The science and technology of gelatin. Herausgegeben von A. G. Ward u. A. Courts, XVI und 564 Seiten mit zahlreichen Abb. u. Tab. (Academic Press London, New York, San Francisco 1977). Preis: 18,00 £; 39,50 $. Food/Nahrung 22(4), 444–445 (1978)
H. Shah, F. Yusof, Gelatin as an ingredient in food and pharmaceutical products: an Islamic perspective. Adv. Environ. Biol. 8(3), 7 (2014)
C. Pena, K. de la Caba, A. Eceiza, R. Ruseckaite, I. Mondragon, Enhancing water repellence and mechanical properties of gelatin films by tannin addition. Bioresour. Technol. 101(17), 6836–6842 (2010)
R. Schrieber, H. Gareis, Gelatine Handbook: Theory and Industrial Practice (New York, Wiley, 2007)
R. Kaintura, P. Sharma, S. Singh, K. Rawat, P.R. Solanki, Gelatin nanoparticles as a delivery system for proteins. J. Nanomed. Res. 2(1), 00018 (2015)
K. Rawat, P.R. Solanki, K. Arora, H.B. Bohidar, Response of gelatin modified electrode towards sensing of different metabolites. Appl. Biochem. Biotechnol. 174(3), 1032–1042 (2014)
A. Mahapatro, D. Singh, Biodegradable nanoparticles are excellent vehicle for site directed in-vivo delivery of drugs and vaccines. J. Nanobiotechnol. 9(1), 55 (2011)
K. Singh, A. Mishra, Gelatin nanoparticle: preparation, characterization and application in drug delivery. Int. J. Pharm. Sci. Res. 5(6), 2149–2157 (2014)
L. Jeong, W.H. Park, Preparation and characterization of gelatin nanofibers containing silver nanoparticles. Int. J. Mol. Sci. 15(4), 6857–6879 (2014)
S. Heydarkhan-Hagvall, K. Schenke-Layland, A.P. Dhanasopon, F. Rofail, H. Smith, B.M. Wu, R. Shemin, R.E. Beygui, W.R. MacLellan, Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering. Biomaterials 29(19), 2907–2914 (2008)
S. Azarmi, Y. Huang, H. Chen, S. McQuarrie, D. Abrams, W. Roa, W.H. Finlay, G.G. Miller, R. Lobenberg, Optimization of a two-step desolvation method for preparing gelatin nanoparticles and cell uptake studies in 143B osteosarcoma cancer cells. J. Pharm. Pharm. Sci. Publ. Can. Soc. Pharm. Sci. Soc. Can. Des Sci. Pharm. 9(1), 124–132 (2006)
B. Mohanty, V.K. Aswal, J. Kohlbrecher, H.B. Bohidar, Synthesis of gelatin nanoparticles via simple coacervation. J. Surface Sci. Technol. 21(3–4), 12 (2005)
C. Pinto Reis, R.J. Neufeld, A.J. Ribeiro, F. Veiga, Nanoencapsulation I. Methods for preparation of drug-loaded polymeric nanoparticles. Nanomed. Nanotechnol. Biol. Med. 2(1), 8–21 (2006)
A.O. Elzoghby, Gelatin-based nanoparticles as drug and gene delivery systems: reviewing three decades of research. J. Controlled Release 172(3), 1075–1091 (2013)
G. Kaul, M. Amiji, Biodistribution and targeting potential of poly (ethylene glycol)-modified gelatin nanoparticles in subcutaneous murine tumor model. J. Drug Target. 12(9–10), 585–591 (2004)
Z. Lu, T.K. Yeh, M. Tsai, J.L. Au, M.G. Wientjes, Paclitaxel-loaded gelatin nanoparticles for intravesical bladder cancer therapy. Clin. Cancer Res. Official J. Am. Assoc. Cancer Res. 10(22), 7677–7684 (2004)
J. Madan, N. Dhiman, S. Sardana, R. Aneja, R. Chandra, A. Katyal, Long-circulating poly (ethylene glycol)-grafted gelatin nanoparticles customized for intracellular delivery of noscapine: preparation, in-vitro characterization, structure elucidation, pharmacokinetics, and cytotoxicity analyses. Anti-cancer Drugs 22(6), 543–555 (2011)
E. Leo, M. Angela Vandelli, R. Cameroni, F. Forni, Doxorubicin-loaded gelatin nanoparticles stabilized by glutaraldehyde: involvement of the drug in the cross-linking process. Int. J. Pharm. 155(1), 75–82 (1997)
M.G. Cascone, L. Lazzeri, C. Carmignani, Z. Zhu, Gelatin nanoparticles produced by a simple W/O emulsion as delivery system for methotrexate. J. Mater. Sci. Mater. Med. 13(5), 523–526 (2002)
A.K. Bajpai, J. Choubey, In vitro release dynamics of an anticancer drug from swellable gelatin nanoparticles. J. Appl. Polym. Sci. 101(4), 2320–2332 (2006)
W.-M. Li, D.-M. Liu, S.-Y. Chen, Amphiphilically-modified gelatin nanoparticles: self-assembly behavior, controlled biodegradability, and rapid cellular uptake for intracellular drug delivery. J. Mater. Chem. 21(33), 12381–12388 (2011)
Y.-W. Won, S.-M. Yoon, C.H. Sonn, K.-M. Lee, Y.-H. Kim, Nano self-assembly of recombinant human gelatin conjugated with α-tocopheryl succinate for Hsp90 inhibitor, 17-AAG, delivery. ACS Nano 5(5), 3839–3848 (2011)
H. Ai, S. Jones, Y. Lvov, Biomedical applications of electrostatic layer-by-layer nano-assembly of polymers, enzymes, and nanoparticles. Cell Biochem. Biophys. 39(1), 23–43 (2003)
A. Saxena, K. Sachin, H.B. Bohidar, A.K. Verma, Effect of molecular weight heterogeneity on drug encapsulation efficiency of gelatin nano-particles. Colloids Surf., B 45(1), 42–48 (2005)
S. Karthikeyan, N. Rajendra Prasad, A. Ganamani, E. Balamurugan, Anticancer activity of resveratrol-loaded gelatin nanoparticles on NCI-H460 non-small cell lung cancer cells. Biomed. Prev. Nutr. 3(1), 64–73 (2013)
D. Ding, Z. Zhu, Q. Liu, J. Wang, Y. Hu, X. Jiang, B. Liu, Cisplatin-loaded gelatin-poly (acrylic acid) nanoparticles: synthesis, antitumor efficiency in vivo and penetration in tumors. Eur. J. Pharm. Biopharm. 79(1), 142–149 (2011)
S. Harsha, Pharmaceutical suspension containing both immediate/sustained-release amoxicillin-loaded gelatin nanoparticles: preparation and in vitro characterization. Drug Des. Dev. Ther. 7, 1027–1033 (2013)
N. Kuntworbe, R. Al-Kassas, Design and in vitro haemolytic evaluation of cryptolepine hydrochloride-loaded gelatine nanoparticles as a novel approach for the treatment of malaria. AAPS PharmSciTech 13(2), 568–581 (2012)
L.-L. Li, J.-H. Xu, G.-B. Qi, X. Zhao, F. Yu, H. Wang, Core-shell supramolecular gelatin nanoparticles for adaptive and “on-demand” antibiotic delivery. ACS Nano 8(5), 4975–4983 (2014)
B. Azimi, P. Nourpanah, M. Rabiee, S. Arbab, Producing gelatin nanoparticles as delivery system for bovine serum albumin. Iran. Biomed. J. 18(1), 34 (2014)
C. Coester, P. Nayyar, J. Samuel, In vitro uptake of gelatin nanoparticles by murine dendritic cells and their intracellular localisation. Eur. J. Pharm. Biopharm. 62(3), 306–314 (2006)
M.S. Sudheesh, S.P. Vyas, D.V. Kohli, Nanoparticle-based immunopotentiation via tetanus toxoid-loaded gelatin and aminated gelatin nanoparticles. Drug Delivery 18(5), 320–330 (2011)
F.M. Chen, Z.W. Ma, G.Y. Dong, Z.F. Wu, Composite glycidyl methacrylated dextran (Dex-GMA)/gelatin nanoparticles for localized protein delivery. Acta Pharmacol. Sin. 30(4), 485–493 (2009)
G. Kaul, M. Amiji, Tumor-targeted gene delivery using poly (ethylene glycol)-modified gelatin nanoparticles: in vitro and in vivo studies. Pharm. Res. 22(6), 951–961 (2005)
G. Kaul, M. Amiji, Long-circulating poly (ethylene glycol)-modified gelatin nanoparticles for intracellular delivery. Pharm. Res. 19(7), 1061–1067 (2002)
P. Magadala, M. Amiji, Epidermal growth factor receptor-targeted gelatin-based engineered nanocarriers for DNA delivery and transfection in human pancreatic cancer cells. AAPS J. 10(4), 565–576 (2008)
K. Zwiorek, C. Bourquin, J. Battiany, G. Winter, S. Endres, G. Hartmann, C. Coester, Delivery by cationic gelatin nanoparticles strongly increases the immunostimulatory effects of CpG oligonucleotides. Pharm. Res. 25(3), 551–562 (2008)
G. Kaul, C. Lee-Parsons, M. Amiji, Poly (ethylene glycol)-modified gelatin nanoparticles for intracellular delivery. Pharm. Eng. 23(5), 108–117 (2003)
S. Kommareddy, M. Amiji, Poly (ethylene glycol)-modified thiolated gelatin nanoparticles for glutathione-responsive intracellular DNA delivery. Nanomed. Nanotechnol. Biol. Med. 3(1), 32–42 (2007)
J. Vandervoort, A. Ludwig, Preparation and evaluation of drug-loaded gelatin nanoparticles for topical ophthalmic use. Eur. J. Pharm. Biopharm. 57(2), 251–261 (2004)
G. Konat Zorzi, L. Contreras-Ruiz, J.E. Párraga, A. López-García, R. Romero Bello, Y. Diebold, B. Seijo, A. Sánchez, Expression of MUC5AC in ocular surface epithelial cells using cationized gelatin nanoparticles. Mol. Pharm. 8(5), 1783–1788 (2011)
G.K. Zorzi, J.E. Párraga, B. Seijo, A. Sánchez, Hybrid nanoparticle design based on cationized gelatin and the polyanions dextran sulfate and chondroitin sulfate for ocular gene therapy. Macromol. Biosci. 11(7), 905–913 (2011)
J.S. Patil, S. Sarasija, Pulmonary drug delivery strategies: a concise, systematic review. Lung India Official Organ Indian Chest Soc. 29(1), 44–49 (2012)
C.-L. Tseng, W.-Y. Su, K.-C. Yen, K.-C. Yang, F.-H. Lin, The use of biotinylated-EGF-modified gelatin nanoparticle carrier to enhance cisplatin accumulation in cancerous lungs via inhalation. Biomaterials 30(20), 3476–3485 (2009)
J.O.H. Sham, Y. Zhang, W.H. Finlay, W.H. Roa, R. Löbenberg, Formulation and characterization of spray-dried powders containing nanoparticles for aerosol delivery to the lung. Int. J. Pharm. 269(2), 457–467 (2004)
S. Fuchs, J. Klier, A. May, G. Winter, C. Coester, H. Gehlen, Towards an inhalative in vivo application of immunomodulating gelatin nanoparticles in horse-related preformulation studies. J. Microencapsul. 29(7), 615–625 (2012)
J. Klier, S. Fuchs, A. May, U. Schillinger, C. Plank, G. Winter, H. Gehlen, C. Coester, A nebulized gelatin nanoparticle-based CpG formulation is effective in immunotherapy of allergic horses. Pharm. Res. 29(6), 1650–1657 (2012)
Y.-C. Chen, S.-H. Yu, G.-J. Tsai, D.-W. Tang, F.-L. Mi, Y.-P. Peng, Novel technology for the preparation of self-assembled catechin/gelatin nanoparticles and their characterization. J. Agric. Food Chem. 58(11), 6728–6734 (2010)
T.G. Shutava, S.S. Balkundi, P. Vangala, J.J. Steffan, R.L. Bigelow, J.A. Cardelli, D.P. O’Neal, Y.M. Lvov, Layer-by-layer-coated gelatin nanoparticles as a vehicle for delivery of natural polyphenols. ACS Nano 3(7), 1877–1885 (2009)
H. Zhou, X. Sun, L. Zhang, P. Zhang, J. Li, Y.-N. Liu, Fabrication of biopolymeric complex coacervation core micelles for efficient tea polyphenol delivery via a green process. Langmuir 28(41), 14553–14561 (2012)
T. Zou, S.S. Percival, Q. Cheng, Z. Li, C.A. Rowe, L. Gu, Preparation, characterization, and induction of cell apoptosis of cocoa procyanidins–gelatin–chitosan nanoparticles. Eur. J. Pharm. Biopharm. 82(1), 36–42 (2012)
Z. Gan, T. Zhang, Y. Liu, D. Wu, Temperature-triggered enzyme immobilization and release based on cross-linked gelatin nanoparticles. PLoS ONE 7(10), e47154 (2012)
S. Palanisamy, S. Cheemalapati, S.-M. Chen, Enzymatic glucose biosensor based on multiwalled carbon nanotubes-zinc oxide composite. Int. J. Electrochem. Sci. 7, 8394 (2012)
M.C. Chang, C.-C. Ko, W.H. Douglas, Preparation of hydroxyapatite-gelatin nanocomposite. Biomaterials 24(17), 2853–2862 (2003)
C.-K. Chiu, J. Ferreira, T.-J. Luo, H. Geng, F.-C. Lin, C.-C. Ko, Direct scaffolding of biomimetic hydroxyapatite-gelatin nanocomposites using aminosilane cross-linker for bone regeneration. J. Mater. Sci. Mater. Med. 23(9), 2115–2126 (2012)
X. Yang, L. Zhu, S. Tada, D. Zhou, T. Kitajima, T. Isoshima, Y. Yoshida, M. Nakamura, W. Yan, Y. Ito, Mussel-inspired human gelatin nanocoating for creating biologically adhesive surfaces. Int. J. Nanomed. 9, 2753–2765 (2014)
T. Taguchi, Y. Endo, Crosslinking liposomes/cells using cholesteryl group-modified tilapia gelatin. Int. J. Mol. Sci. 15(7), 13123–13134 (2014)
Y. Yang, H. Tang, A. Köwitsch, K. Mäder, G. Hause, J. Ulrich, T. Groth, Novel mineralized heparin–gelatin nanoparticles for potential application in tissue engineering of bone. J. Mater. Sci. Mater. Med. 25(3), 669–680 (2014)
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2016 Springer India
About this chapter
Cite this chapter
Sharma, P.P., Sharma, A., Solanki, P.R. (2016). Recent Trends of Gelatin Nanoparticles in Biomedical Applications. In: Husain, M., Khan, Z. (eds) Advances in Nanomaterials. Advanced Structured Materials, vol 79. Springer, New Delhi. https://doi.org/10.1007/978-81-322-2668-0_10
Download citation
DOI: https://doi.org/10.1007/978-81-322-2668-0_10
Published:
Publisher Name: Springer, New Delhi
Print ISBN: 978-81-322-2666-6
Online ISBN: 978-81-322-2668-0
eBook Packages: EngineeringEngineering (R0)